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Immunochemical Approaches to Coastal, Estuarine and Oceanographic Questions

  • Conference proceedings
  • © 1988

Overview

Editors:
  1. Clarice M. Yentsch

    1. Bigelow Laboratory for Ocean Sciences, W. Boothbay Harbor, USA

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  2. Frances C. Mague

    1. Bigelow Laboratory for Ocean Sciences, W. Boothbay Harbor, USA

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  3. Paul K. Horan

    1. Dept. of Cell Biology, Smith, Kline and French Laboratory, Swedeland, USA

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Part of the book series: Coastal and Estuarine Studies (COASTAL, volume 25)

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Table of contents (28 papers)

  1. Front Matter

    Pages N2-XVI
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  2. Background of the Immune System

    1. Front Matter

      Pages 1-1
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    2. Understanding The Immune System

      • Office of Research Reporting and Public Response National Institute of Allergy and Infectious Diseases National Institutes of Health
      Pages 2-21

  4. Techniques

    1. Front Matter

      Pages 73-73
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    2. The Production of Monospecific Antisera to Soft-Bottom Benthic Taxa

      • Eugene D. Gallagher, Peter A. Jumars, Gary L. Taghon
      Pages 74-98

    3. Preparation and use of Monospecific Antisera to Capitella SP. I.

      • Eugene D. Gallagher, J. P. Grassle, D. D. Trueblood, M. G. Gleason
      Pages 99-99

    4. Cell Affinity Chromatography for a Marine Nitrifying Bacterium

      • U. Heyman, B. Heyman, Bess B. Ward
      Pages 100-116

    5. Application of Immunoblotting for Dietary Analysis

      • Gregory Zagursky, Robert J. Feller
      Pages 117-129

    6. Two-Dimensional Electrophoresis with Silver Staining: A Sensitive Method For Analyzing Complex Protein Mixtures

      • Marcia F. Goldfarb
      Pages 130-133

    7. Brevetoxins and Binding: Sodium Channels Versus Antibodies

      • Daniel G. Baden, Thomas J. Mende, Alina M. Szmant
      Pages 134-143

    8. Preparation of Model Haptens to Express Common Epitopes of PSP Toxins

      • Patrick E. Guire, Peter H. Duquette, Richard A. Amos, C. Jerome Behrens, Mark W. Josephson, Ross P. Chambers
      Pages 145-154

    9. Monoclonal Antibodies (Monoabs) to Ciguatoxin and Related Polyethers

      • Y. Hokama, S. A. A. Honda, M. N. Kobayashi, L. K. Nakagawa, L. K. Shirai, J. T. Miyahara
      Pages 155-166

    10. Immunofluorescent Reagents: Preparation and Analysis

      • John F. Daley
      Pages 167-183

    11. Flow Cytometry and Immunofluorescence in Aquatic Sciences

      • Terry L. Cucci, David Robins
      Pages 184-193

    12. Detection Schemes for Antigen-Antibody Reactions

      • Diether J. Recktenwald, Chia-Huei Chen Chen, Laura Chiu, Morgan Conrad
      Pages 194-206

  5. Applications

    1. Front Matter

      Pages 207-207
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    2. Identification of Marine Chroococcoid Cyanobacteria by Immunofluorescence

      • Lisa Campbell
      Pages 208-229
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Keywords

About this book

This volume represents a collection of pioneering papers introducing immunochemical techniques to the aquatic sciences. It is the product of a workshop entitled "Immunochemical Approaches to Coastal, Estuarine and Oceanographic Questions" held at the University of Southern Maine, Portland, Oct. 5-7, 1986. Funding from many sources made this workshop possible, and is gratefully acknowledged. The reader will note great variability in both the quality and scope of the papers which we have divided 'into four sections: I. Background material on the immune system (borrowed from a recent NIH publication) II. Background material related to aquatic sciences III. Techniques IV. Applications Readers familiar with terminology are directed to start with the original contributions related to aquatic sciences, page 21. Some of the contributions are abstracts only. Others are preliminary, for which we are grateful to the authors for their willingness to share recent sometimes tentative findings with a broader community. Some offer novel and tempting insights, but as they exist, lack adequate standards and controls. Others are thorough and represent major scientific contributions. The promise rests in generating and working with reagents of great specificity enabling orders of magnitude improvements in the sensitivity of our measurements. Authors also address the problems and limitations. We thank the numerous authors and reviewers. In support of every successful endeavor is someone of unusual savvy. For us, this has been David A. Phinney. His leadership, wisdom and wit have been important to the introduction of flow cytometry/sorting and immunochemistry into aquatic research.

Editors and Affiliations

  • Bigelow Laboratory for Ocean Sciences, W. Boothbay Harbor, USA

    Clarice M. Yentsch, Frances C. Mague

  • Dept. of Cell Biology, Smith, Kline and French Laboratory, Swedeland, USA

    Paul K. Horan

Bibliographic Information

  • Book Title: Immunochemical Approaches to Coastal, Estuarine and Oceanographic Questions

  • Editors: Clarice M. Yentsch, Frances C. Mague, Paul K. Horan

  • Series Title: Coastal and Estuarine Studies

  • DOI: https://doi.org/10.1007/978-1-4684-7642-2

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media New York 1988

  • Softcover ISBN: 978-0-387-96894-0Published: 28 November 1988

  • eBook ISBN: 978-1-4684-7642-2Published: 13 March 2013

  • Series ISSN: 0724-5890

  • Edition Number: 1

  • Number of Pages: XVI, 399

  • Number of Illustrations: 31 b/w illustrations, 23 illustrations in colour

  • Topics: Biochemistry, general, Infectious Diseases, Immunology, Ecology, Microbiology

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