Skip to main content
SpringerLink
Log in
Birkhäuser

Gene Therapeutics

Methods and Applications of Direct Gene Transfer

  • Book
  • © 1994

Overview

Editors:
  1. Jon A. Wolff

    1. Department of Pediatrics, University of Wisconsin Medical School, Madison, USA
      Department of Medical Genetics, University of Wisconsin Medical School, Madison, USA
      Department of Neurology, University of Wisconsin Medical School, Madison, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (22 chapters)

  1. Front Matter

    Pages i-xviii
    Download chapter PDF

  3. Methods and Mechanisms

    1. Front Matter

      Pages 81-81
      Download chapter PDF

    2. Possible Mechanisms of DNA Uptake in Skeletal Muscle

      • Martin E. Dowty, Jon A. Wolff
      Pages 82-98

    3. Receptor-Mediated Gene Delivery Employing Adenovirus-Polylysine-DNA Complexes

      • David T. Curiel
      Pages 99-117

    4. Gene Transfer in Mammalian Cells Using Liposomes as Carriers

      • Arun Singhal, Leaf Huang
      Pages 118-142

    5. In Vivo Gene Therapy via Receptor-Mediated DNA Delivery

      • Henry C. Chiou, George L. Spitalny, June R. Merwin, Mark A. Findeis
      Pages 143-156

    6. Calcium Phosphate-Mediated DNA Transfection

      • Patricia L. Chang
      Pages 157-179

    7. Cellular Internalization of Oligodeoxynucleotides

      • Leonard M. Neckers
      Pages 180-192

    8. Gene Transfer via Particle Bombardment: Applications of the Accell Gene Gun

      • Ning-Sun Yang, Carolyn De Luna, Liang Cheng
      Pages 193-209

    9. Electrically-Induced DNA Transfer into Cells. Electrotransfection in Vivo

      • Sergei I. Sukharev, Alexander V. Titomirov, Vadim A. Klenchin
      Pages 210-232

  4. Applications

    1. Front Matter

      Pages 233-233
      Download chapter PDF

    2. Pharmacokinetic Considerations in the Use of Genes as Pharmaceuticals

      • Fred D. Ledley
      Pages 235-244

    3. Virus-Mediated Genetic Treatment of Rodent Gliomas

      • E. Antonio Chiocca, Julie K. Andersen, Yoshiaki Takamiya, Robert L. Martuza, Xandra O. Breakefield
      Pages 245-262

    4. Gene Therapy for Adenosine Deaminase Deficiency and Malignant Solid Tumors

      • Kenneth W. Culver, R. Michael Blaese
      Pages 263-280

    5. Development of Herpes Simplex Virus Vectors for Gene Transfer to the Central Nervous System

      • Joseph C. Glorioso, Neal A. DeLuca, William F. Goins, David J. Fink
      Pages 281-302
Back to top

Keywords

About this book

During the first half century of genetics, coinciding with the first half of this cen­ tury, geneticists dreamt of the repair of genetic disease by altering or replacing defective genes. H. J. Muller wrote of the great advantages of mutations, "nanoneedles" in his apt term, for delicately probing physiological and chemical processes. In the same spirit, genes could be used to provide treatments of needle point delicacy. Yet, during this period no realistic possibility appeared; it remained but a dream. The situation changed abruptly at the half century. Microbial genetics and its offshoot, cell culture genetics, provided the route. Pneumococcus transformation showed that exogenous DNA could become a permanent part of the genome; yet attempts to reproduce this in animals produced a few tantalizing hints of success, but mostly failures. Transduction, using a virus as mediator, offered a better op­ portunity. The fITSt reproducible in vivo gene therapy in a whole animal came in 1981. This was in Drosophila, with a transposable element as carrier. Flies were "cured" of a mutant eye color by incorporation of the normal allele, and the effect was transmissible, foreshadowing not only somatic, but germ line gene therapy. At the same time, retroviruses carrying human genes were found to be ex­ tremely efficient in transferring their contents to the chromosomes of cultured cells.

Editors and Affiliations

  • Department of Pediatrics, University of Wisconsin Medical School, Madison, USA

    Jon A. Wolff

  • Department of Medical Genetics, University of Wisconsin Medical School, Madison, USA

    Jon A. Wolff

  • Department of Neurology, University of Wisconsin Medical School, Madison, USA

    Jon A. Wolff

Bibliographic Information

  • Book Title: Gene Therapeutics

  • Book Subtitle: Methods and Applications of Direct Gene Transfer

  • Editors: Jon A. Wolff

  • DOI: https://doi.org/10.1007/978-1-4684-6822-9

  • Publisher: Birkhäuser Boston, MA

  • eBook Packages: Springer Book Archive

  • Copyright Information: Birkhäuser Boston 1994

  • Softcover ISBN: 978-1-4684-6824-3Published: 26 February 2012

  • eBook ISBN: 978-1-4684-6822-9Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: 417

  • Topics: Gene Therapy, Medicine/Public Health, general, Human Genetics

Publish with us

Policies and ethics

Back to top

Search

Navigation