Cytotoxic Cells: Recognition, Effector Function, Generation, and Methods

1. Front Matter

   Pages i-xiv

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2. Introduction and Overview

   1. Front Matter

      Pages 1-1

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   2. Introductory Remarks

      • Herman N. Eisen

      Pages 3-4

   3. T-Cell-Mediated Cytotoxicity: A Historical Note

      • Jean-Charles Cerottini

      Pages 5-8

   4. Overview of CTL-Target Adhesion and Other Critical Events in the Cytotoxic Mechanism

      • Eric Martz

      Pages 9-45

3. Target Cell Recognition

   1. Front Matter

      Pages 47-47

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   2. αβ T-Cell Receptor Repertoires Among Cytotoxic and Helper T Lymphocytes

      • David M. Kranz, Benjamin Tjoa

      Pages 49-57

   3. Class I MHC/Peptide/ β ₂-Microglobulin Interactions:The Basis of Cytotoxic T-Cell Recognition

      • David H. Margulies, Lisa F. Boyd, Maripat Corr, Rosemarie D. Hunziker, Sergei Khilko, Steven Kozlowski et al.

      Pages 58-64

   4. The Role of CD8-Class I Interactions in CTL Function

      • Anne M. O’Rourke, Matthew F. Mescher

      Pages 65-71

   5. Interactions Between CD2 and T-Cell Receptor Isoforms in CTL Function

      • Shigeo Koyasu, Ellis L. Reinherz

      Pages 72-83

   6. Triggering Structures on NK Cells

      • Lewis L. Lanier, Joseph H. Phillips

      Pages 84-95

   7. Targeted Cellular Cytotoxicity

      • David M. Segal, Carolina R. Jost, Andrew J. T. George

      Pages 96-110

4. Generation of Cytotoxic Cells

   1. Front Matter

      Pages 111-111

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   2. Immunobiology and Molecular Characteristics of Peritoneal Exudate Cytotoxic T Lymphocytes (PEL), Their In Vivo IL-2-Dependent Blasts and IL-2-Independent Cytolytic Hybridomas

      • Gideon Berke, Dalia Rosen, Denise Ronen, Barbara Schick

      Pages 113-127

   3. Regulatory Effects of Cytokines on the Generation of CTL and LAK Cells

      • Mark R. Alderson, Michael B. Widmer

      Pages 128-137

   4. IL-2-Independent Activation of LAK Cells by a Heterodimeric Cytokine, Interleukin-12

      • Maurice K. Gately, Aimee G. Wolitzky, Phyllis M. Quinn, Richard Chizzonite

      Pages 138-144

   5. Immunobiology of β ₂ -Microglobulin-Deficient Mice

      • J. A. Frelinger, D. G. Quinn, D. Muller

      Pages 145-150

5. Molecular Mechanisms of Cellular Cytotoxicity

   1. Front Matter

      Pages 151-151

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   2. The Granule Exocytosis Model for Lymphocyte Cytotoxicity and Its Relevance to Target Cell DNA Breakdown

      • Pierre A. Henkart, Mark P. Hayes, John W. Shiver

      Pages 153-165

   3. Subpopulations of Cytotoxic T Lymphocytes with Different Cytotoxic Mechanisms

      • David W. Lancki, Maureen McKisic, Frank W. Fitch

      Pages 166-177

Our motivation for putting together this book was the need for a single source reference that could be used as an introduction to cell-mediated cytotoxicity for newcomers to this field, such as students and fellows beginning work in our laboratories. At present no such book is available, and we felt that it would be useful as a teaching tool and as a way of conveying our enthusiasm about recent progress in the cytotoxicity field to our colleagues in allied areas. It was with some hesitation that we approached our colleagues with the proposal for this book, and we were pleased to find them very supportive of the idea and willing to participate. We thought it important to broaden the scope of the book to include historical, molecular, cell biological, and clinical aspects of cell-mediated cytotoxicity. To our knowledge this is the first book on cell-mediated cytotoxicity with such a broad scope. Historically, studies on cellular cytotoxicity were part of cellular immunology from its origin. One development of tremendous import was the advent of the 51 Cr assay, which allowed this arm of the immune response to be measured easily and quantitatively. Thus, a readout of this effector pathway is available within a few hours; other immune effector functions can take days or even longer to assay, and the assays are often less quantitative.

• DNA
• antibody
• antigen
• apoptosis
• cell
• clinical trial
• cytokine
• cytotoxicity
• immune response
• immunology
• infection
• molecular biology
• parasite
• pharmacology
• virus
• infectious diseases

• Laboratory of Immunology, Biochemistry and Immunopharmacology Unit, NIH-NIAID, Bethesda, USA

  Michail V. Sitkovsky

• Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, USA

  Pierre A. Henkart

Policies and ethics