Enzymology and Molecular Biology of Carbonyl Metabolism 3

1. Front Matter

   Pages i-ix

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2. Aldehyde Dehydrogenase

   1. Aldehyde Dehydrogenases: What can be Learned from a Baker’s Dozen Sequences?

      • Ronald Lindahl, John Hempel

      Pages 1-8

   2. Rat Class 3 Aldehyde Dehydrogenase: Crystals and Preliminary Analysis

      • John Hempel, John P. Rose, Ingrid Kuo, Ronald Lindahl, Bi-Cheng Wang

      Pages 9-11

   3. Probing the Active Site of Aldehyde Dehydrogenase by Site Directed Mutagenesis

      • Henry Weiner, Jaume Farrés, Thomas T. Y. Wang, Suzanne J. Cunningham, Chao-Feng Zheng, Ghiorghis Ghenbot

      Pages 13-17

   4. Localization of Cysteine 302 at the Active Site of Aldehyde Dehydrogenase

      • Regina Pietruszko, Erich Blatter, Darryl P. Abriola, Glenn Prestwich

      Pages 19-30

   5. PH Effects on Cytoplasmic Aldehyde Dehydrogenase from Sheep Liver

      • Paul D. Buckley, Rosemary L. Motion, Leonard F. Blackwell, Jeremy P. Hill

      Pages 31-41

   6. Biochemical, Immunological, and Molecular Characterization of a “High KM” Aldehyde Dehydrogenase

      • Rolf Eckey, Rüdiger Timmann, John Hempel, Dharam P. Agarwal, H. Werner Goedde

      Pages 43-52

   7. Purification and Properties of Baboon Corneal Aldehyde Dehydrogenase: Proposed UVR Protective Role

      • Elizabeth M. Algar, Mahin Abedinia, John L. VandeBerg, Roger S. Holmes

      Pages 53-60

   8. A Polymorphism in the Rat Liver Mitochondrial ALDH2 Gene is Associated with Alcohol Drinking Behavior

      • L. Carr, B. Mellencamp, D. Crabb, L. Lumeng, T.-K. Li

      Pages 61-65

   9. Aldehydic Products of Lipid Peroxidation: Substrates or Inhibitors of Hepatic Aldehyde Dehydrogenase?

      • D. R. Petersen, J. J. Hjelle, D. Y. Mitchell

      Pages 67-73

   10. Metabolism of 4-Hydroxynonenal in Hepatoma Cell Lines

       • R. A. Canuto, G. Muzio, A. M. Bassi, M. E. Biocca, G. Poli, H. Esterbauer et al.

       Pages 75-84

   11. Role of Aldehyde Dehydrogenase (ALDH) in the Detoxication of Cyclophosphamide (CP) in Rat Embryos

       • Philip E. Mirkes, Aaron Ellison, Sally A. Little

       Pages 85-95

   12. Human and Mouse Hepatic Aldehyde Dehydrogenases Important in the Biotransformation of Cyclophosphamide and the Retinoids

       • N. E. Sladek, P. A. Dockham, M. O. Lee

       Pages 97-104

   13. A Paradigm for Aldehyde Oxidation: Histidinol Dehydrogenase

       • Charles Grubmeyer

       Pages 105-112

3. Aldo-Keto Reductase

   1. Structural Studies of Pig Lens Aldose Reductase: Reversible Dimerization of the Enzyme

      • Jean-Michel Rondeau, Dino Moras, Frédérique Tête, Alberto Podjarny, Alain Van Dorsselaer, Jean-Marc Reymann et al.

      Pages 113-118

   2. cDNA Cloning and Expression of Human Aldose Reductase

      • Chihiro Nishimura, Yoshiharu Matsuura, Tsuyoshi Tanimoto, Takashi Yamaoka, Tai Akera, T. Geoffrey Flynn

      Pages 119-127

   3. In Vitro Expression of Human Placental Aldose Reductase in Escherichia Coli

      • Deborah Carper, Sanai Sato, Susan Old, Stephen Chung, Peter F. Kador

      Pages 129-138

   4. Osmotic Stress Induces Aldose Reductase in Glomerular Endothelial Cells

      • Thomas C. Hohman, Deborah Carper, Sarmila Dasgupta, Masayuki Kaneko

      Pages 139-152

   5. Purification of Aldose and Aldehyde Reductases from Dog Kidney

      • Sanai Sato

      Pages 153-163

   6. Study on Dihydrodiol Dehydrogenase (I) Molecular Forms of the Enzyme and the Presence of a Dihydrodiol Specific Enzyme in Bovine Liver Cytosol

      • Tohru Nishinaka, Tomoyuki Terada, Toshifumi Umemura, Hirofumi Nanjo, Tadashi Mizoguchi, Tsutomu Nishihara

      Pages 165-175

The Fifth International Workshop on the Enzymology and Molecular Biology of Carbonyl Metabolism was held at Purdue University in June, 1990. This represents the fifth time that I had the privilege of organizing the scientific program. It was the first time that I actually hosted the meeting. I wish to salute my four previous co-organizers and the thousands of scientists who have hosted other meetings. It is much easier to arrange the scientific program and edit the proceedings. No local organization could occur without the help of ones research group and, in this case, my wife. I sincerely thank Esther and my research group for their advise and help. At this Workshop, similar to the preceeding ones, much new information was presented. It was apparent how molecular biological techniques were influencing the direction of the research on the three families of enzymes discussed. It also was apparent that not all biochemical problems could be solved by using these techniques. Many of the presentations showed how important advances still could be made using more traditional biochemical approaches.

• biology
• carbon
• enzymes
• enzymology
• metabolism
• molecular biology
• research

• Department of Biochemistry, Purdue University, West Lafayette, USA

  Henry Weiner

• Chemisches Zentrallabor, Inselspital, Bern, Switzerland

  Bendicht Wermuth

• Indiana University School of Medicine and Veterans Administration Medical Center, Indianapolis, USA

  David W. Crabb

Policies and ethics