Ultrastructure Techniques for Microorganisms

1. Front Matter

   Pages i-xiv

   PDF

2. Preparation of Microfungi for Scanning Electron Microscopy

   • Garry T. Cole

   Pages 1-44

3. Low-Temperature Scanning Electron Microscopy

   • Alan Beckett, Nick D. Read

   Pages 45-86

4. Effects of Specimen Preparation on the Apparent Ultrastructure of Microorganisms

   • William J. Todd

   Pages 87-100

5. Secrets of Successful Embedding, Sectioning, and Imaging

   • H. C. Aldrich, H. H. Mollenhauer

   Pages 101-132

6. Computer-Aided Reconstruction of Serial Sections

   • David L. Balkwill

   Pages 133-160

7. Electron Microscopy of Nucleic Acids

   • Claude F. Garon

   Pages 161-181

8. Freeze-Substitution of Fungi

   • Harvey C. Hoch

   Pages 183-212

9. Freeze-Fracture (-Etch) Electron Microscopy

   • Russell L. Chapman, L. Andrew Staehelin

   Pages 213-240

10. Preparation of Freeze-Dried Specimens for Electron Microscopy

    • Thomas H. Giddings Jr., George P. Wray

    Pages 241-265

11. Low-Temperature Embedding

    • Barbara L. Armbruster, Edward Kellenberger

    Pages 267-295

12. High-Voltage Electron Microscopy

    • Sandra A. Nierzwicki-Bauer

    Pages 297-331

13. Computer Analysis of Ordered Microbiological Objects

    • Murray Stewart

    Pages 333-364

14. Digitizing and Quantitation

    • Edwin S. Boatman

    Pages 365-398

15. Localization of Carbohydrate-Containing Molecules

    • Gregory W. Erdos

    Pages 399-420

16. Cytochemical Techniques for the Subcellular Localization of Enzymes in Microorganisms

    • Martha J. Powell

    Pages 421-445

17. Localization of Nucleic Acids in Sections

    • H. C. Aldrich, Sylvia E. Coleman

    Pages 447-467

18. Colloidal Gold Labels for Immunocytochemical Analysis of Microbes

    • John Smit, William J. Todd

    Pages 469-516

19. X-Ray Microanalysis

    • H. C. Aldrich

    Pages 517-525

20. Back Matter

    Pages 527-533

    PDF

The modem microbiologist is often a real specialist who has difficulty under­ standing and applying many of the techniques beyond those in his or her own immediate field. On the other hand, most benefits to modem microbiology are obtained when a broad spectrum of scientific approaches can be focused on a problem. In early studies, electron microscopy was pivotal in understanding bacterial and viral morphology, and we still feel that we will understand a disease better if we have seen an electron micrograph of the causative agent. Today, because there is an increased awareness of the need to understand the rela­ tionships between microbial structure and function, the electron microscope is still one of the most important tools microbiologists can use for detailed analysis of microorganisms. Often, however, the aforementioned modem microbiologist still thinks of ultrastructure as involving negative staining or ultrathin sectioning in order to get a look at the shape of a "bug. " Many of the newer ultrastructure techniques, such as gold-labeled antibody localization, freeze-fracture, X-ray microanalysis, enzyme localization, and even scanning electron microscopy, are poorly under­ stood by, and therefore forbidding to, the average microbiologist. Even many cell biologists admit to having difficulty staying in touch with current develop­ ments in the fast-moving field of electron microscopy techniques.

• Seen
• antibody
• biology
• cell
• microbiology
• microorganism
• morphology

• Department of Microbiology and Cell Science, University of Florida, Gainesville, USA

  Henry C. Aldrich

• Department of Veterinary Science Louisiana Agricultural Experiment Station, Louisiana State University Agricultural Center, Baton Rouge, USA

  William J. Todd

• Department of Veterinary Microbiology and Parasitology School of Veterinary Medicine, Louisiana State University, Baton Rouge, USA

  William J. Todd

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