Skip to main content
SpringerLink
Log in

Enzyme-Mediated Immunoassay

  • Book
  • © 1985

Overview

Editors:
  1. T. T. Ngo

    1. University of California, Irvine, Irvine, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. H. M. Lenhoff

    1. University of California, Irvine, Irvine, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (26 chapters)

  1. Front Matter

    Pages i-vii
    Download chapter PDF

  2. Introduction

    1. Introduction

      • T. T. Ngo, H. M. Lenhoff
      Pages 1-2

  3. Enzyme Mediated Immunoassay: An Overview

    1. Enzyme Mediated Immunoassay: An Overview

      • T. T. Ngo
      Pages 3-32

  4. Separation-Free (Homogeneous) Enzyme Mediated Immunoassays

    1. Separation-Free Enzyme Immunoassay for Haptens

      • Anna Jaklitsch
      Pages 33-55

    2. Enzyme Modulator as Label in Separation-Free Immunoassays: Enzyme Modulator Mediated Immunoassay (EMMIA)

      • T. T. Ngo
      Pages 57-72

    3. Prosthetic Group Labeled Enzyme Immunoassay

      • T. T. Ngo
      Pages 73-84

    4. Fluorogenic Substrate Labeled Separation-Free Enzyme Mediated Immunoassays for Haptens and Macromolecules

      • That T. Ngo, Raphael C. Wong
      Pages 85-109

    5. Immunoassays Based on Conformational Restriction of the Enzyme Label Induced by the Antibody

      • T. T. Ngo, H. M. Lenhoff
      Pages 111-119

    6. Nonseparation Enzyme Immunoassays for Macromolecules

      • Ian Gibbons
      Pages 121-143

    7. Liposome-Entrapped Enzyme Mediated Immunoassays

      • William J. Litchfield, J. William Freytag
      Pages 145-153

    8. Test Strip Enzyme Immunoassay

      • David J. Litman
      Pages 155-190

    9. Separation-Free Enzyme Fluorescence Immunoassay by Continuous Flow Injection Analysis

      • Tim A. Kelly
      Pages 191-201

  5. Seperation-Required (Heterogenous) Enzyme Immunoassay

    1. Separation-Required (Heterogenous) Enzyme Immunoassay for Haptens and Antigens

      • Jim C. Standefer
      Pages 203-222

    2. Enzyme Immunoassay of Antibody

      • Seymour P. Halbert, Tsue-Ming Lin
      Pages 223-239

    3. The Amplified Enzyme-Linked Immunosorbent Assay (a-ELISA)

      • J. E. Butler, J. H. Peterman, T. E. Koertge
      Pages 241-276

    4. Affinity Column Mediated Immunoenzymometric Assays

      • J. William Freytag
      Pages 277-289

    5. Steric Hindrance Enzyme Immunoassay (Sheia)

      • Albert Castro, Nobuo Monji
      Pages 291-298

    6. Maleimide Derivative of Hapten for Enzyme Coupling in EIA

      • Nobuo Monji, Albert Castro
      Pages 299-310

    7. Enzyme Immunoassays Using Tagged Enzyme-Ligand Conjugates

      • T. T. Ngo, H. M. Lenhoff
      Pages 313-323

    8. Fluorimetric Measurements in Enzyme Immunoassays

      • Kristin H. Milby
      Pages 325-341
Back to top

Keywords

About this book

T. T. Ngo and H. M. Lenhoff Department of Developmental and Cell Biology University of California, Irvine, CA 92717 In 1959, Yalow and Berson used insulin labeled with radioactive iodine to develop a quantitative immunological method for determining the amount of insulin in human plasma. Their method depends upon ~ competition between insulin labeled with radioactive iodine (II 1) and unlabeled insulin from plasma for a fixed and limited number of specific binding sites on the antibody to insulin. The amount of the labeled insulin bound to the antibody is inversely proportional to the amount of insulin in the plasma sample. Their method, which is so elegantly simple in concept, is made possible by the ability to detect with ease extremely low levels of radioactivity, and by the exquisite specificity of an antibody capable of specifically binding the analyte. Such a combination of sensitivity and specificity is the basis of this versatile analytical tool called radioimmunoassay (RIA). Twelve years later, Engvall and Perlmann (1971) and Van Weemen and Schuurs (1971) independently introduced the use of enzymes as another category of sensitive and even more versatile labels for use in immunoassays. Engvall and Perlmann (l971) coined the term ELISA, which stands for Enzyme Linked Immunosorbent Assay.

Editors and Affiliations

  • University of California, Irvine, Irvine, USA

    T. T. Ngo, H. M. Lenhoff

Bibliographic Information

  • Book Title: Enzyme-Mediated Immunoassay

  • Editors: T. T. Ngo, H. M. Lenhoff

  • DOI: https://doi.org/10.1007/978-1-4684-5012-5

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: Plenum Press, New York 1985

  • Softcover ISBN: 978-1-4684-5014-9Published: 13 March 2012

  • eBook ISBN: 978-1-4684-5012-5Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: 498

  • Topics: Biochemistry, general, Biotechnology

Publish with us

Policies and ethics

Back to top

Search

Navigation