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Techniques in Somatic Cell Genetics

  • Book
  • © 1982

Overview

Editors:
  1. Jerry W. Shay

    1. The University of Texas Health Science Center at Dallas, Dallas, USA

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Table of contents (36 chapters)

  1. Front Matter

    Pages i-xxvi
    Download chapter PDF

  2. Selection of Purine and Pyrimidine Nucleoside Analog Resistance in Mammalian Cells

    • John Morrow
    Pages 1-9

  3. Techniques for Using HAT Selection in Somatic Cell Genetics

    • W. N. Choy, T. V. Gopalakrishnan, J. W. Littlefield
    Pages 11-21

  4. Techniques for Decreasing the Toxicity of Polyethylene Glycol

    • W. Edward Mercer, Renato Baserga
    Pages 23-34

  5. The Use of Dimethyl Sulfoxide in Mammalian Cell Fusion

    • Thomas H. Norwood, Carol J. Zeigler
    Pages 35-45

  6. The Selection of Heterokaryons and Cell Hybrids Using the Biochemical Inhibitors Iodoacetamide and Diethylpyrocarbonate

    • Woodring Erik Wright
    Pages 47-65

  7. Techniques for Enucleation of Mammalian Cells

    • George E. Veomett
    Pages 67-79

  8. Nonselective Isolation of Fibroblast Heterokaryons, Hybrids, and Cybrids by Flow Sorting

    • Johan F. Jongkind, Anton Verkerk
    Pages 81-100

  9. Techniques for Monitoring Cell Fusion: The Synthesis and Use of Fluorescent Vital Probes (R18, F18)

    • Paul M. Keller, Stanley Person
    Pages 101-109

  10. Inheritance of Oligomycin Resistance in Tissue Culture Cells

    • Jerome M. Eisenstadt, Mary C. Kuhns
    Pages 111-121

  11. Cytoplasmic Inheritance of Rutamycin Resistance in Mammalian Cells

    • Godfrey S. Getz, Kathleen L. Kornafel
    Pages 123-137

  12. Erythromycin Resistance in Human Cells

    • Claus-Jens Doersen, Eric J. Stanbridge
    Pages 139-157

  13. Cytoplasmic Inheritance of Chloramphenicol Resistance in Mammalian Cells

    • Douglas C. Wallace
    Pages 159-187

  14. The Influence of Cytoplast-to-Cell Ratio on Cybrid Formation

    • Clive L. Bunn
    Pages 189-201

  15. Transformation of Mitochondrially Coded Genes into Mammalian Cells Using Intact Mitochondria and Mitochondrial DNA

    • Mike A. Clark, Tim L. Reudelhuber, Jerry W. Shay
    Pages 203-210

  16. Mitochondrial Influences in Hybrid Cells

    • Michael L. Ziegler
    Pages 211-219

  17. Shedding of Tumor Cell Surface Membranes

    • Andrejs Liepins
    Pages 221-235

  18. Production of Microcytospheres

    • Gerd G. Maul, Josef Weibel
    Pages 237-243

  19. Isolation and Characterization of Mitoplasts

    • Potu N. Rao, Prasad S. Sunkara, Abdullatif A. Al-Bader
    Pages 245-254

  20. Nuclear Transplantation with Mammalian Cells

    • Margaret J. Hightower, Joseph J. Lucas
    Pages 255-267
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Keywords

About this book

Somatic cell genetics is an exciting and rapidly expanding field of research. Since descriptions of the major experimental techniques in the field are scattered throughout various journals and other publications, there is a real need for a single reference source for both established investigators and students in the field. In addition, technical reports are frequently abridged such that many researchers are discouraged from attempting to adopt the appropriate methodology. This book, therefore, describes in detail the many recent technical advances in such areas of somatic cell genetics as transfer mediated by liposomes, erythrocyte ghosts, chromosomes, micro cells, mito­ chondria, and isolated nuclear DNA. These techniques have increased our understanding of the organization and regulation of eukaryotic cells. The production of antibiotic-resistant cell lines and their use in studying cytoplasmic inheritance are also included. Evidence for the cytoplasmic regulation of nuclear gene expression in eukaryotic cells is rapidly accumu­ lating following the characterization of cytoplasmic mutations. The produc­ tion of nuclear-coded mutations, their use in standard cell hybridization, and recent advances in techniques for fusing whole cells or cell components are also described.

Editors and Affiliations

  • The University of Texas Health Science Center at Dallas, Dallas, USA

    Jerry W. Shay

Bibliographic Information

  • Book Title: Techniques in Somatic Cell Genetics

  • Editors: Jerry W. Shay

  • DOI: https://doi.org/10.1007/978-1-4684-4271-7

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: Plenum Press, New York 1982

  • Softcover ISBN: 978-1-4684-4273-1Published: 26 April 2012

  • eBook ISBN: 978-1-4684-4271-7Published: 21 November 2013

  • Edition Number: 1

  • Number of Pages: XXVI, 568

  • Number of Illustrations: 95 b/w illustrations

  • Topics: Human Genetics

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