Techniques in Somatic Cell Genetics

1. Front Matter

   Pages i-xxvi

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2. Selection of Purine and Pyrimidine Nucleoside Analog Resistance in Mammalian Cells

   • John Morrow

   Pages 1-9

3. Techniques for Using HAT Selection in Somatic Cell Genetics

   • W. N. Choy, T. V. Gopalakrishnan, J. W. Littlefield

   Pages 11-21

4. Techniques for Decreasing the Toxicity of Polyethylene Glycol

   • W. Edward Mercer, Renato Baserga

   Pages 23-34

5. The Use of Dimethyl Sulfoxide in Mammalian Cell Fusion

   • Thomas H. Norwood, Carol J. Zeigler

   Pages 35-45

6. The Selection of Heterokaryons and Cell Hybrids Using the Biochemical Inhibitors Iodoacetamide and Diethylpyrocarbonate

   • Woodring Erik Wright

   Pages 47-65

7. Techniques for Enucleation of Mammalian Cells

   • George E. Veomett

   Pages 67-79

8. Nonselective Isolation of Fibroblast Heterokaryons, Hybrids, and Cybrids by Flow Sorting

   • Johan F. Jongkind, Anton Verkerk

   Pages 81-100

9. Techniques for Monitoring Cell Fusion: The Synthesis and Use of Fluorescent Vital Probes (R18, F18)

   • Paul M. Keller, Stanley Person

   Pages 101-109

10. Inheritance of Oligomycin Resistance in Tissue Culture Cells

    • Jerome M. Eisenstadt, Mary C. Kuhns

    Pages 111-121

11. Cytoplasmic Inheritance of Rutamycin Resistance in Mammalian Cells

    • Godfrey S. Getz, Kathleen L. Kornafel

    Pages 123-137

12. Erythromycin Resistance in Human Cells

    • Claus-Jens Doersen, Eric J. Stanbridge

    Pages 139-157

13. Cytoplasmic Inheritance of Chloramphenicol Resistance in Mammalian Cells

    • Douglas C. Wallace

    Pages 159-187

14. The Influence of Cytoplast-to-Cell Ratio on Cybrid Formation

    • Clive L. Bunn

    Pages 189-201

15. Transformation of Mitochondrially Coded Genes into Mammalian Cells Using Intact Mitochondria and Mitochondrial DNA

    • Mike A. Clark, Tim L. Reudelhuber, Jerry W. Shay

    Pages 203-210

16. Mitochondrial Influences in Hybrid Cells

    • Michael L. Ziegler

    Pages 211-219

17. Shedding of Tumor Cell Surface Membranes

    • Andrejs Liepins

    Pages 221-235

18. Production of Microcytospheres

    • Gerd G. Maul, Josef Weibel

    Pages 237-243

19. Isolation and Characterization of Mitoplasts

    • Potu N. Rao, Prasad S. Sunkara, Abdullatif A. Al-Bader

    Pages 245-254

20. Nuclear Transplantation with Mammalian Cells

    • Margaret J. Hightower, Joseph J. Lucas

    Pages 255-267

Somatic cell genetics is an exciting and rapidly expanding field of research. Since descriptions of the major experimental techniques in the field are scattered throughout various journals and other publications, there is a real need for a single reference source for both established investigators and students in the field. In addition, technical reports are frequently abridged such that many researchers are discouraged from attempting to adopt the appropriate methodology. This book, therefore, describes in detail the many recent technical advances in such areas of somatic cell genetics as transfer mediated by liposomes, erythrocyte ghosts, chromosomes, micro cells, mito­ chondria, and isolated nuclear DNA. These techniques have increased our understanding of the organization and regulation of eukaryotic cells. The production of antibiotic-resistant cell lines and their use in studying cytoplasmic inheritance are also included. Evidence for the cytoplasmic regulation of nuclear gene expression in eukaryotic cells is rapidly accumu­ lating following the characterization of cytoplasmic mutations. The produc­ tion of nuclear-coded mutations, their use in standard cell hybridization, and recent advances in techniques for fusing whole cells or cell components are also described.

• DNA
• chromosome
• gene expression
• genetics
• hybridization

• The University of Texas Health Science Center at Dallas, Dallas, USA

  Jerry W. Shay

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