Genetic Engineering of Microorganisms for Chemicals

1. Front Matter

   Pages i-xiii

   PDF

2. Introduction

   • Irwin C. Gunsalus

   Pages 1-2

3. Welcoming Remarks

   • Alexander Hollaender

   Pages 3-4

4. Plasmids as Vectors for Gene Cloning: Past, Present, and Future Use

   • Donald Helinski

   Pages 5-24

5. Strategies: Techniques and Molecular Systems Chairman’s Introduction

   • Rollin D. Hotchkiss

   Pages 25-27

6. Cloning Vectors Derived from Bacterial Plasmids

   • Michael Bittner, Daniel Vapnek

   Pages 29-49

7. Expression of a Foreign Procaryotic Gene in Bacillus Subtilis

   • Paul S. Lovett, Donna M. Williams, Elizabeth J. Duvall

   Pages 51-57

8. Fusion of Microbial Protoplasts: Problems and Perspectives

   • Lajos Alföldi

   Pages 59-71

9. Strategies: Models and Problems

   • Stanley Dagley

   Pages 73-74

10. Baker’s Yeast: A Successful Industrial Microorganism is Now a Favorable Host for Molecular Cloning

    • John F. Scott

    Pages 75-86

11. Transformation of Neurospora Crassa Utilizing Recombinant Plasmid DNA

    • Mary E. Case

    Pages 87-100

12. Perspectives for the Genetic Engineering of Hydrocarbon Oxidizing Bacteria

    • James A. Shapiro, A. Carbit, S. Benson, M. Caruso, R. Laux, R. Meyer et al.

    Pages 101-102

13. Photosynthesis and Cloning in Cyanobacteria

    • Louis A. Sherman, James Guikema

    Pages 103-131

14. Models for Genetic Manipulation of Actinomycetes

    • John H. Coats

    Pages 133-142

15. The Regulation of Yeast Gene Expression by Multiple Control Elements

    • Terrance G. Cooper

    Pages 143-161

16. Strategies for Cloning in Bacillus Subtilis

    • James A. Hoch, Ahn Nguyen, Eugenio Ferrari

    Pages 163-173

17. Genetic and Molecular Studies of the Regulation of Atypical Citrate Utilization and Variable Vi Antigen Expression in Enteric Bacteria

    • L. S. Baron, D. J. Kopecko, S. M. McCowen, N. J. Snellings, E. M. Johnson, W. C. Reid et al.

    Pages 175-194

18. Molecular Cloning in the Streptococci

    • Francis L. Macrina, Janet Ash Tobian, Kevin R. Jones, R. Paul Evans

    Pages 195-210

19. Genetic Engineering on Microorganisms for Chemicals: Diversity of Genetic and Biochemical Traits of Pseudomonads

    • Douglas W. Ribbons, Peter A. Williams

    Pages 211-232

20. Beginning Genetics with Methanogens

    • John N. Reeve, Nancy J. Trun, Paul T. Hamilton

    Pages 233-244

The normal course of most biologically catalyzed processes is tightly regulated at the genetic and physiological levels. The regulatory mechanisms are diverse, sometimes redundant, and it is becoming increasingly apparent that, at the genetic level, the range of mechanisms may be limited only by the permutations and combina­ tions available. For each microbial cell, evolution appears to have resulted in maximized advantage to that cell, achieving regulatory balance. Genetic engineering encompasses our attempts to perturb the genetic regulation of a cell so that we may obtain desired other than normal outcomes, such as increased product formation, or new product formation. Following the groundwork established by a preceding symposium (Trends in the Biology of Fermentations for Fuels and Chemicals, Brookhaven National Laboratory, December 1980), the initial planning for this conference envisioned the juxtaposition of molecular genetic expertise and microbial biochemical expertise. The resultant interaction should encourage new and extended ideas for the improve­ ment of strains and for the generation of new regulatory combina­ tions to enhance microbial chemical production from cheap and abundant (including waste) substrates. The interaction should also demonstrate that new discoveries at the basic level remain essential to progress in genetic engineering. New genetic regulatory combina­ tions require new studies of physiology and biochemistry to assure understanding and control of the system. New biochemical reactions necessitate new studies of genetic and regulatory interaction.

• Fermentation
• biochemistry
• chemistry
• genetic engineering
• physiology
• reaction

• Associated Universities, Inc., USA

  Alexander Hollaender

• Department of Microbiology, University of Illinois, Champaign, USA

  Ralph D. DeMoss

Policies and ethics