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Excitable Cells in Tissue Culture

  • Book
  • © 1981

Overview

Editors:
  1. Phillip G. Nelson

    1. Laboratory of Developmental Neurobiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, USA

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  2. Melvyn Lieberman

    1. Department of Physiology, Duke University Medical Center, Durham, USA

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    You can also search for this editor in PubMed   Google Scholar

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Table of contents (12 chapters)

  1. Front Matter

    Pages i-xvii
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  2. The Expression of Cholinergic and Adrenergic Properties by Autonomic Neurons in Tissue Culture

    • Harold Burton, Richard P. Bunge
    Pages 1-37

  3. Electrophysiological and Structural Studies of Neurons in Dissociated Cell Cultures of the Central Nervous System

    • Phillip G. Nelson, Elaine A. Neale, Robert L. MacDonald
    Pages 39-80

  4. Neuropharmacology of Spinal Cord Neurons in Primary Dissociated Cell Culture

    • Robert L. MacDonald, Jeffery L. Barker
    Pages 81-110

  5. Voltage Clamp Techniques Applied to Cultured Skeletal Muscle and Spinal Neurons

    • Thomas G. Smith Jr., Jeffery L. Barker, Bruce M. Smith, Theodore R. Colburn
    Pages 111-136

  6. Membrane Noise Analysis

    • Harold Lecar, Frederick Sachs
    Pages 137-172

  7. Nerve Cells in Clonal Systems

    • Yosef Kimhi
    Pages 173-245

  8. Electrophysiology of Clonal Nerve Cell Lines

    • Ilan Spector
    Pages 247-277

  9. Studies of Voltage-Sensitive Sodium Channels in Cultured Cells Using Ion-Flux and Ligand-Binding Methods

    • William A. Catterall
    Pages 279-317

  10. Electrophysiological Properties of Developing Skeletal Muscle Cells in Culture

    • Yoshiaki Kidokoro
    Pages 319-339

  11. The Physiology of Smooth Muscle Cells in Tissue Culture

    • Robert D. Purves
    Pages 341-357

  12. Calcium Exchange in Myocardial Tissue Culture

    • Glenn A. Langer
    Pages 359-377

  13. Cardiac Muscle with Controlled Geometry

    • Melvyn Lieberman, C. Russell Horres, Norikazu Shigeto, Lisa Ebihara, James F. Aiton, Edward A. Johnson
    Pages 379-408

  14. Back Matter

    Pages 409-422
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Keywords

About this book

The tissue culture approach to the study of membrane properties of excitable cells has progressed beyond the technical problems of culture methodology. Recent developments have fostered substantive contributions in research con­ cerned with the physiology, pharmacology, and biophysics of cell membranes in tissue culture. The scope of this volume is related to the application of tissue culture methodology to developmental processes and cellular mechanisms of electrical and chemical excitability. The major emphasis will be on the body of new biological information made available by the analytic possibilities inherent in the tissue culture systems. Naturally occurring preparations of excitable cells are frequently of suf­ ficient morphological complexity to compromise the analysis of the data obtained from them. Some of the limitations associated with dissected prepa­ rations have to do with the direct visualization of and access to the cell(s) in question and maintenance of steady-state conditions for prolonged periods of time. Since preparations in tissue culture can circumvent these problems, it is feasible to analyze the properties of identifiable cells, grown either singly or in prescribed geometries, as well as to follow the development of cellular inter­ actions. A crucial consideration in the use of cultured preparations is that they must faithfully capture the phenomenon of interest to the investigator. This and other potential limitations on the methodology are of necessary concern in the present volume.

Editors and Affiliations

  • Laboratory of Developmental Neurobiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, USA

    Phillip G. Nelson

  • Department of Physiology, Duke University Medical Center, Durham, USA

    Melvyn Lieberman

Bibliographic Information

  • Book Title: Excitable Cells in Tissue Culture

  • Editors: Phillip G. Nelson, Melvyn Lieberman

  • DOI: https://doi.org/10.1007/978-1-4684-3803-1

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: Plenum Press, New York 1981

  • Softcover ISBN: 978-1-4684-3805-5Published: 14 April 2012

  • eBook ISBN: 978-1-4684-3803-1Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: 440

  • Number of Illustrations: 89 b/w illustrations

  • Topics: Animal Anatomy / Morphology / Histology

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