Research Methods in Neurochemistry

1. Front Matter

   Pages i-xvi

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2. Properties of Intact Neural Tissues

   1. Front Matter

      Pages 1-1

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   2. Isolation of Axolemma-Enriched Fractions from Mammalian CNS

      • George H. DeVries

      Pages 3-38

   3. Understanding Receptor-Binding Assays

      • Milt Titeler

      Pages 39-73

   4. Isolation of Postsynaptic Densities from Cerebral Cortex

      • Philip Siekevitz

      Pages 75-89

   5. Clearance of Radiolabeled Substances by Brain after Arterial Injection Using a Diffusible Internal Standard

      • William H. Oldendorf

      Pages 91-112

   6. Oligodendroglia: Bulk Isolation and Maintenance as Suspension Cultures

      • Shirley E. Poduslo

      Pages 113-125

3. Components of Neural Tissues—Enzymes

   1. Front Matter

      Pages 127-127

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   2. Assay Methods, Purification, and Characterization of L-Glutamate Decarboxylase and GABA-Transaminase

      • Jang-Yen Wu, Y. Y. Thomas Su, Dominic M. K. Lam, A. Schousboe, Obi Chude

      Pages 129-177

   3. Intracellular Phospholipid Transfer Proteins in the Central Nervous System

      • M. J. Brammer

      Pages 179-199

   4. Methods for Studying Lipid-Mediated Glycosyltransferases Involved in the Assembly of Glycoproteins in Nervous Tissue

      • C. J. Waechter, M. G. Scher

      Pages 201-231

4. Components of Neural Tissues—Proteins

   1. Front Matter

      Pages 233-233

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   2. Isolation of 14–3–2 Protein, Neuron-Specific Enolase, and S100 Protein: Methods for Assay of Messenger RNA Activity

      • Claire E. Zomzely-Neurath, William A. Walker

      Pages 235-279

   3. Contractile Proteins of Brain Tissue

      • Saul Puszkin, William J. Schook

      Pages 281-310

5. Back Matter

   Pages 311-318

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This fifth volume of Research Methods in Neurochemistry represents a milestone in that it marks almost a decade since the inception of the series. Over these ten years there has been an almost exponential growth in neuro­ chemistry accompanied by numerous technical developments. This is the justification for our series; inevitably we have only been able to cover a frac­ tion of the methodological innovations of the last decade, but we have tried as much as possible to create a balance between the different approaches and philosophies in the study of the chemical basis of brain function. Thus our original format of grouping chapters under various headings-for instance, studies in intact tissues as distinct from studies describing constituents and isolated enzymes-appears to be justified. Studies on whole animals or tissues retaining cellular organization are vital in providing insights into the neurochemical mechanism underlying functional processes; at the same time, the eventual understanding of function can only be attained on the basis of knowledge of the molecular architecture of the tissue. In the present volume, Oldendorfs chapter on the transport of radiolabeled metabolites across the blood-brain barrier illustrates one side of this equation, whereas Poduslo's chapter on the separation of oligo­ dendroglia! cells provides new information on the role of these cells in myelogenesis and the distinctive chemical composition of glia as compared to neurons.

• brain
• neurochemistry
• neurons

• Center for Neurochemistry, Rockland Research Institute, New York, USA

  Neville Marks

• Department of Biochemistry, Institute of Psychiatry, University of London, London, Great Britain

  Richard Rodnight

Policies and ethics