Bioenergetics of the Cell: Quantitative Aspects

1. Front Matter

   Pages i-1

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2. What do we not know of cellular bioenergetics? — a general view on the state of the art

   1. Front Matter

      Pages 3-3

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   2. What do we not know of cellular bioenergetics? — a general view on the state of the art

      • Valdur A. Saks, Renée Ventura-Clapier, Xavier Leverve, André Rossi, Michel Rigoulet

      Pages 3-9

3. Bioenergetics of Mitochondria: in Vitro and in vivo Studies

   1. Front Matter

      Pages 11-11

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   2. Top-down elasticity analysis and its application to energy metabolism in isolated mitochondria and intact cells

      • Martin D. Brand

      Pages 13-20

   3. A model of O·2 -generation in the complex III of the electron transport chain

      • O. V. Demin, B. N. Kholodenko, V. P. Skulachev

      Pages 21-33

   4. Quantitative analysis of some mechanisms affecting the yield of oxidative phosphorylation: Dependence upon both fluxes and forces

      • Michel Rigoulet, Xavier Leverve, Eric Fontaine, Rachid Ouhabi, Bernard Guérin

      Pages 35-52

   5. Oxidative phosphorylation in intact hepatocytes: Quantitative characterization of the mechanisms of change in efficiency and cellular consequences

      • Xavier Leverve, Brigitte Sibille, Anne Devin, Marie-Astrid Piquet, Pascal Espié, Michel Rigoulet

      Pages 53-65

   6. Yeast mitochondrial metabolism: From in vitro to in situ quantitative study

      • Nicole Avéret, Valérie Fitton, Odile Bunoust, Michel Rigoulet, Bernard Guérin

      Pages 67-79

   7. Permeabilized cell and skinned fiber techniques in studies of mitochondrial function in vivo

      • Valdur A. Saks, Vladimir I. Veksler, Andrei V. Kuznetsov, Laurence Kay, Peeter Sikk, Toomas Tiivel et al.

      Pages 81-100

   8. Cytoskeleton and mitochondrial morphology and function

      • L. Rappaport, P. Oliviero, J. L. Samuel

      Pages 101-105

   9. Energetics of swelling in isolated hepatocytes: A comprehensive study

      • Anne Devin, Pascal Espié, Bernard Guérin, Michel Rigoulet

      Pages 107-121

4. Energy Transfer Networks: Molecular Physiology of Kinases, Lessons from Transgenic Mice, Mathematical Theories

   1. Front Matter

      Pages 123-123

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   2. Functional aspects of the X-ray structure of mitochondrial creatine kinase: A molecular physiology approach

      • Uwe Schlattner, Michael Forstner, Michael Eder, Olaf Stachowiak, Karin Fritz-Wolf, Theo Wallimann

      Pages 125-140

   3. Oligomeric state and membrane binding behaviour of creatine kinase isoenzymes: Implications for cellular function and mitochondrial structure

      • Olaf Stachowiak, Uwe Schlattner, Max Dolder, Theo Wallimann

      Pages 141-151

   4. Molecular characterization of the creatine kinases and some historical perspectives

      • Wenning Qin, Zaza Khuchua, Judy Cheng, Jaime Boero, R. Mark Payne, Arnold W. Strauss

      Pages 153-167

   5. Adenylate kinase: Kinetic behavior in intact cells indicates it is integral to multiple cellular processes

      • Petras P. Dzeja, Robert J. Zeleznikar, Nelson D. Goldberg

      Pages 169-182

   6. Cytoarchitectural and metabolic adaptations in muscles with mitochondrial and cytosolic creatine kinase deficiencies

      • Karen Steeghs, Frank Oerlemans, Arnold de Haan, Arend Heerschap, Lia Verdoodt, Martine de Bie et al.

      Pages 183-194

   7. In situ measurements of creatine kinase flux by NMR. The lessons from bioengineered mice

      • Klaas Nicolay, Ferdi A. van Dorsten, Torsten Reese, Marijn J. Kruiskamp, Johannes F. Gellerich, Cees J. A. van Echteld

      Pages 195-208

   8. Mathematical model of compartmentalized energy transfer: Its use for analysis and interpretation of 31 P-NMR studies of isolated heart of creatine kinase deficient mice

      • Mayis K. Aliev, F. A. van Dorsten, M. G. Nederhoff, C. J. A. van Echteld, Vladimir Veksler, Klaas Nicolay et al.

      Pages 209-229

This volume continues the discussion of the problems of in vivo and in vitro. The recently solved X-ray structure of the mitochondrial creatine kinase and its molecular biology cellular bioenergetics - the tradition we started in 1994 by publication of the focused issue of Molecular and Cellular are analyzed with respect to its molecular physiology and Biochemistry, volume 133/134 and a book 'Cellular Bio­ functional coupling to the adenine nucleotide translocase, as energetics: role of coupled creatine kinases' edited by V. Saks well as its participation, together with the adenylate kinase and R. Ventura-Clapier and published by Kluwer Publishers, system, in intracellular energy transfer. The results of the Dordrecht -Boston. In the present volume, use of quantitative studies of creatine kinase deficient transgenic mice are methods of studies of organized metabolic systems, such as summarized and analyzed by using mathematical models of mathematical modeling and Metabolic Control Analysis, for the compartmentalized energy transfer, thus combining two investigation of the problems of bioenergetics of the cell is powerful new methods of the research. All these results, described together with presentation of new experimental together with the physiological and NMR data on the cardiac results. The following central problems of the cellular bio­ metabolic and mitochondrial responses to work-load changes energetics are the focus of the discussions: the mechanisms concord to the concept of metabolic networks of energy of regulation of oxidative phosphorylation in the cells in vivo transfer and feedback regulation.

• Calcium
• Nucleotide
• biology
• enzyme
• enzymes
• metabolism
• mitochondria
• molecular biology
• phosphorylation

• Laboratories of Bioenergetics, Institute of Chemical and Biological Physics, Tallinn, Estonia

  Valdur A. Saks

• Laboratory of Bioenergetics, Joseph Fourier University, Grenoble, France

  Valdur A. Saks, Xavier Leverve, André Rossi

• Cellular and Molecular Cardiology, Chatenay-Malabry, France

  Renée Ventura-Clapier

• Laboratory of Bioenergetics, Institute of Biochemistry and Cellular Genetics CNRS, Bordeaux, France

  Michel Rigoulet

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