Overview
- Editors:
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Leslie J. Fairbairn
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Christie CRC Research Centre Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK
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Nydia G. Testa
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Christie CRC Research Centre Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK
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Table of contents (14 chapters)
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- N. G. Testa, E. A. de Wynter, J. Hows
Pages 1-12
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- Mary Collins, Colin Porter
Pages 57-88
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- L. J. Fairbairn, L. S. Lashford
Pages 203-218
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- Michael Antoniou, Frank Grosveld
Pages 219-242
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- A. K. Stewart, I. D. Dubé, R. G. Hawley
Pages 243-268
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- J. A. Rafferty, L. J. Fairbairn
Pages 293-312
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- Xinqiang Li, Flossie Wong-Staal, Anthony D. Ho, Ping Law
Pages 313-330
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- Rosa Maria Diaz, Richard G. Vile
Pages 331-350
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- Heather L. Davis, Cynthia L. Brazolot Millan
Pages 351-376
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Back Matter
Pages 377-380
About this book
Since the first concepts of gene therapy were formulated, the hemopoietic system has been considered the most natural first target tissue for genetic manipulation. The reasons for this include the fact that a very large number of inherited disorders (including some of the most common disorders, such as the hemoglobinopathies) are disorders of the hemopoietic system, and the large amount of experience in hematopoietic transplantation biology. The consequence of this resulted in the first clinical trial of gene therapy in 1989, where two children suffering from severe combined immune deficiency (ADA-SCID) were transplanted with T-cells express ing adenosine deaminase (the defective enzyme in patients with this disorder). The partial success of this treatment was perhaps responsible for undue optimism among those proposing other gene therapy treatments within the hematopoietic system, and it has since become clear that there are a number of technical and biological difficulties to overcome before hematopoietic gene therapy becomes a mainstream therapeutic strategy. The chapters in this book evaluate the need for gene therapy in the hematopoietic system, discuss how efficient gene transfer and expression can be achieved in the target cells, highlight areas of difficulty to be addressed, and examine a number of potential applications of the gene therapy approach. The book begins with a chapter by Testa and colleagues, discussing the various sources of hematopoietic cells for both transplantation and gene therapy.