Methods in Mammary Gland Biology and Breast Cancer Research

1. Front Matter

   Pages i-xvi

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2. In Vivo Model Systems

   1. Front Matter

      Pages 1-1

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   2. Mouse Models for Mammary Cancer

      • Daniel Medina

      Pages 3-17

   3. Methods for the Induction of Mammary Carcinogenesis in the Rat Using either 7,12-Dimethylbenz[α]anthracene or 1-Methyl-1-Nitrosourea

      • Henry J. Thompson

      Pages 19-29

   4. A Comparison of the Salient Features of Mouse, Rat, and Human Mammary Tumorigenesis

      • Daniel Medina, Henry J. Thompson

      Pages 31-36

   5. Xenograft Models of Human Breast Cancer Lines and of the MCF10AT Model of Human Premalignant, Proliferative Breast Disease

      • Fred R. Miller, Gloria H. Heppner

      Pages 37-50

   6. Implantation and Characterization of Human Breast Carcinomas in SCID Mice

      • Yan Xu, Stephen B. Edge, Thelma C. Hurd, Elizabeth A. Repasky, Harry K. Slocum

      Pages 51-63

3. Special Techniques for In Vivo Studies

   1. Front Matter

      Pages 65-65

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   2. The Cleared Mammary Fat Pad and the Transplantation of Mammary Gland Morphological Structures and Cells

      • Lawrence J. T. Young

      Pages 67-74

   3. Preparing Mammary Gland Whole Mounts from Mice

      • Susan B. Rasmussen, Lawrence J. T. Young, Gilbert H. Smith

      Pages 75-85

   4. Slow-Release Plastic Pellets (Elvax) for Localized In Situ Treatments of Mouse and Rat Mammary Tissue

      • Gary B. Silberstein, Charles W. Daniel

      Pages 87-96

   5. Intramammary Delivery of Hormones, Growth Factors, and Cytokines

      • Barbara K. Vonderhaar, Erika Ginsburg

      Pages 97-99

   6. Hormonal Stimulation of the Mouse Mammary Gland

      • Daniel Medina, Frances Kittrell

      Pages 101-107

4. In Vitro Model Systems

   1. Front Matter

      Pages 109-109

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   2. Collagen Gel Method for the Primary Culture of Mouse Mammary Epithelium

      • Walter Imagawa, Jason Yang, Raphael C. Guzman, Satyabrata Nandi

      Pages 111-123

   3. Chemical-Carcinogen-Induced Transformation of Primary Cultures of Mouse Mammary Epithelial Cells Grown inside Collagen Gels

      • Raphael C. Guzman, Walter Imagawa, Jason Yang, Shigeki Miyamoto, Satyabrata Nandi

      Pages 125-135

   4. Establishment of Mouse Mammary Cell Lines

      • Daniel Medina, Frances Kittrell

      Pages 137-145

   5. Whole Organ Culture of the Mouse Mammary Gland

      • Erika Ginsburg, Barbara K. Vonderhaar

      Pages 147-154

   6. Working with the Mouse Mammary End Bud

      • Charles W. Daniel, Gary B. Silberstein

      Pages 155-162

   7. Isolation and Culture of Normal Rat Mammary Epithelial Cells

      • Kathleen M. Darcy, Danilo Zangani, Ping-Ping H. Lee, Margot M. Ip

      Pages 163-175

approaches to the experimental problems that still face us in understanding this most fascinating of organs. Too many people contributed to the completion of this volume to allow acknowledg­ ment of all the individual efforts, but we particularly thank the reviewers whose input into the editorial process was invaluable and the authors of these chapters who revised their text, sometimes more than once, to bring it to the high standards set by the Editors. The Com­ mittee gratefully acknowledges the support ofVysis, Inc. , in the publication of a color figure in Chapter 19, by S. Weber-Hall and Trevor Dale. Finally, we wish to express our heartfelt appreciation to Margot Ip and Bonnie Asch, who worked long and hard to bring this volume to fruition. Margaret C. Neville for the Committee on Mammary Gland Biology Preface One of the most exciting and beneficial developments in research on mammary gland biology and breast cancer has been the influx of increased funding to support this work. This influx, which has been due primarily to the tireless efforts of breast cancer activists to gamer addi­ tional money from various federal and state sources, has led to a rapid expansion of research efforts by attracting numerous new investigators into the field. These new investigators include students, postdoctoral fellows, and scientists from other fields.

• carcinogenesis
• carcinoma
• cell
• cytokines
• embryo
• hormones
• metastasis
• transplantation
• tumorigenesis

• Department of Pharmacology and Therapeutics, Roswell Park Cancer Institute, Buffalo, USA

  Margot M. Ip

• Roswell Park Cancer Research Institute, Buffalo, USA

  Bonnie B. Asch

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