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The Use of Resealed Erythrocytes as Carriers and Bioreactors

  • Book
  • © 1992

Overview

Editors:
  1. Mauro Magnani

    1. University of Urbino, Urbino, Italy

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    You can also search for this editor in PubMed   Google Scholar

  2. John R. DeLoach

    1. Agricultural Research Service Food Animal Protection Research Laboratory, United States Department of Agriculture, College Station, USA

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Advances in Experimental Medicine and Biology (AEMB, volume 326)

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Table of contents (42 chapters)

  1. Front Matter

    Pages i-xiii
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  2. On Red Blood Cells, Hemolysis and Resealed Ghosts

    1. Front Matter

      Pages 1-1
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    2. On Red Blood Cells, Hemolysis and Resealed Ghosts

      • Joseph F. Hoffman
      Pages 1-15

  3. Methods and Procedures for Preparing Resealed Erythrocytes

    1. Front Matter

      Pages 17-17
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    2. IHP Entrapment into Human Erythrocytes: Comparison between Hypotonic Dialysis and DMSO Osmotic Pulse

      • Andrea Mosca, Renata Paleari, Vincenzo Russo, Enrico Rosti, Rita Nano, Andrea Boicelli et al.
      Pages 19-26

    3. Density Gradient Separation of Inositol Hexaphosphate Loaded Red Blood Cells in Various Preparation Conditions

      • Guillemette Bourget, Laurence Boucher, Claude Ropars
      Pages 27-33

    4. Quantitative IHP Determination by 31P - NMR: Proposal for a Standardized Protocol

      • Rita Nano, Andrea Mosca, Renata Paleari, Andrea Boicelli
      Pages 35-39

    5. Determination of Inositol Hexaphosphate (IHP) in Human IHP-Loaded Red Blood Cells by a Simple High Performance Liquid Chromatography Method

      • Stefania Villa, Fabio Rossi, Pier Antonio Biondi, Vincenzo Russo, Tiziano Crimella, Gemino Fiorelli et al.
      Pages 41-49

    6. Determination of Purity of Commercially Available Inositol Hexaphosphate (Phytates) and Preparation of a Reference Material

      • Tiziano Crimella, Stefania Villa, Fabio Rossi, Gemino Fiorelli, Alberto Zanella
      Pages 51-54

    7. Standardization of an Encapsulation System: A Method to Remove Fragile Cells

      • Laura Chiarantini, John R. DeLoach
      Pages 55-62

    8. The Dialysis Encapsulation Process: The Role of Endocytosis

      • Kathleen Andrews, Robert E. Droleskey, Laura Chiarantini, John R. DeLoach
      Pages 63-71

    9. Use of Fluorescent Probes for Describing the Process of Encapsulation by Hypotonic Dialysis

      • Robert E. Droleskey, Kathleen Andrews, Laura Chiarantini, John R. DeLoach
      Pages 73-80

    10. Properties of Hypotonized, Crosslinked and Crosslinked- Permeabilized Rat Erythrocytes as Potential Carrier Systems

      • José Luque, Marina I. Garín, Silvia Sanz, Paloma Ropero, Montserrat Pinilla
      Pages 81-89

    11. Membrane Properties of Senescent and Carrier Human Erythrocytes

      • Maria A. Castellana, Maria R. De Renzis, Giampiero Piccinini, Giampaolo Minetti, Claudio Seppi, Cesare Balduini et al.
      Pages 91-99

    12. Studies with Biotinylated RBC: (1) Use of Flow Cytometry to Determine Posttransfusion Survival and (2) Isolation Using Streptavidin Conjugated Magnetic Beads

      • Vincenzo Russo, Robin Barker-Gear, Robert Gates, Robert Franco
      Pages 101-107

  4. Resealed Erythrocytes as a Tool for Basic Studies

    1. Front Matter

      Pages 109-109
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    2. Resealing of Protein Tyrosine Kinase Substrates into Human Erythrocytes by Rapid Freezing and Thawing in Liquid Nitrogen

      • Marietta Harrison, Chris Isaacson, Prema Rathinavelu, Philip S. Low
      Pages 111-117

    3. The Metabolic Role of Glucose 1,6-P2 in Human Erythrocytes Studied by Encapsulation Procedures

      • Augusto Accorsi, Elena Piatti, Maria Piera Piacentini, Antonio Fazi
      Pages 119-126

    4. The Application of Newer Concepts of Oxygen Transport to the Evaluation of the Potential Utility of Reduced Hb-O2 Affinity

      • Murray Weiner
      Pages 127-131

    5. Single Cell Analysis of Factors Increasing the Survival of Resealed Erythrocytes in the Circulation of Mice

      • Robert A. Schlegel, Katherine Lumley-Sapanski, Patrick Williamson
      Pages 133-138
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Keywords

About this book

Until recently the only biomedical use of erythrocytes was in transfusion medicine to restore a normal oxygen delivery. The development of a technology that permits one to open and reseal erythrocytes has dramatically changed this perspective. Currently, a number of teams have shown that engineered erythrocytes can behave as circulating bioreactors for the degradation of toxic metabolites or the inactivation of xenobiotics, as drug delivery systems, as carriers of antigens of vaccinal interest, and in many others biomedical applications. The technology of opening and resealing the erythrocytes has also been used successfully to investigate several basic aspects of erythrocyte metabolism, survival, pathology, etc. Thus, researchers in this field have an extraordinary opportunity to specifically modify the erythrocytes by the introduction of enzymes that generate new metabolic abilities, antibodies that inactivate single metabolic steps, or metabolites that can influence oxygen delivery and/or other cell properties. Furthermore, the pharmacokinetics of any drug can be potentially manipulated by using the erythrocytes as a delivery system. This book, The Use of Resealed Erythrocytes, is based on the fourth meeting of the "International Society for the Use of Resealed Erythrocytes as Carriers and Bioreactors" (I. S. U. R. E. ), held in Urbino, Italy, in 1991, and examines the most recent applications and developments of this technology.

Editors and Affiliations

  • University of Urbino, Urbino, Italy

    Mauro Magnani

  • Agricultural Research Service Food Animal Protection Research Laboratory, United States Department of Agriculture, College Station, USA

    John R. DeLoach

Bibliographic Information

  • Book Title: The Use of Resealed Erythrocytes as Carriers and Bioreactors

  • Editors: Mauro Magnani, John R. DeLoach

  • Series Title: Advances in Experimental Medicine and Biology

  • DOI: https://doi.org/10.1007/978-1-4615-3030-5

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 1992

  • Hardcover ISBN: 978-0-306-44345-9Published: 31 January 1993

  • Softcover ISBN: 978-1-4613-6321-7Published: 12 November 2012

  • eBook ISBN: 978-1-4615-3030-5Published: 06 December 2012

  • Series ISSN: 0065-2598

  • Series E-ISSN: 2214-8019

  • Edition Number: 1

  • Number of Pages: XIII, 361

  • Topics: Immunology, Biochemistry, general, Biotechnology

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