Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence

1. Front Matter

   Pages i-xi

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2. Microinjection: A Technique to Study Inhibition of HIV-1 Replication Mediated by Antisense RNA and Parvovirus Genes

   • Georg Sczakiel, Ingo Oelze, Karola Rittner

   Pages 1-10

3. How to Make Glass Microtools for the Injection of Isolated Plant Sperm Cells into Embryo SAC Cells Using a Microforge

   • C. J. Keijzer

   Pages 11-17

4. Application of the Firefly Luciferase Reporter Gene to Microinjection Experiments in Xenopus Oocytes

   • Michael R. H. White, Martin Braddock, Elaine D. Byles, Lorene Amet, Alan J. Kingsman, Susan M. Kingsman

   Pages 19-28

5. Application of Confocal Microscopy for the Study of Neuronal Organization in Human Cortical Areas after Microinjection of Lucifer Yellow

   • Pavel V. Belichenko, Annica Dahlstrom, Patrick Sourander

   Pages 29-35

6. The Dye-Transfer Assay Permits the Evaluation of the Modulation of Junctional Communication by Chemicals: Results Obtained Employing a Highly Sensitive Video-Recording System Connected with the Microinjector

   • Giovanna Mazzoleni, Anna Camplani, Paola Telò, Silvia Tanganelli, Giovanni Ragnotti

   Pages 37-43

7. Comparison of Fluorescent Dye Transfer in Intercellular Communication Studies Using Scrape-Loading and Microinjection Techniques

   • E. Honikman-Leban, M. M. Shahin

   Pages 45-52

8. Characterization of the Zea Mays Embryo Sac Using Fluorescent Probes and Microinjection of Lucifer Yellow into the Female Cells

   • E. Matthys-Rochon, C. Digonnet, C. Dumas

   Pages 53-60

9. Fluorescent Probes — Where Do they Go in Cells, and Why?

   • Richard W. Horobin, Fiza Rashid-Doubell

   Pages 61-66

10. Fluorescent Phospholipids in Membrane and Lipoprotein Research

    • A. Hermetter, R. Gorges, J. Loidl, F. Paltauf

    Pages 67-72

11. Selection of Fluorescent Golgi Complex Probes Using Structure-Activity Relationship Models

    • Fiza Rashid-Doubell, Richard W. Horobin

    Pages 73-78

12. Fluorescent Probes for the Assessment of the Subcellular Effects of Chemicals and Disease Processes In Vivo and In Vitro

    • Peter H. Bach, C. Hugh Reynolds, Stephen Brant

    Pages 79-97

13. Whole-Cell Patch-Clamping: Introducing Substances into Cells During Electrical Measurements from the Cell Membrane -A Review of Potential Difficulties in Plant and Animal Cells

    • Bert Van Duijn, Can Ince, Zheng Wang, A. Freek Weidema, Kees R. Libbenga, Dirk L. Ypey

    Pages 99-109

14. Fluorescent Analysis of Replication and Intermediates of Chromatin Folding in Nuclei of Mammalian Cells

    • Gaspar Banfalvi

    Pages 111-119

15. Autofluorescence in Potato Tuber Phellem: Location and Time Course of Appearance During Tuber Formation

    • Theo Hendriks, Jan-Wouter van Eck, Harrie A. Verhoeven

    Pages 121-127

16. Flow Cytometric Assessment of Gentamicin Nephrotoxicity in Established Renal Cell Lines

    • H. Mc Glynn, E. Healy, T. Bedell, M. P. Ryan

    Pages 129-132

17. The Use of the Fluorescent Probe Fura-2 For Intracellular Free Calcium Measurements: Some Methodological Aspects

    • Anneke Wiltink, Arnoud van der Laarse, Nel P. M. Herrmann-Erlee, Joke M. van der Meer, Dirk L. Ypey

    Pages 133-142

18. Studies on the Effects of Peroxisome Proliferators as Novel Ca2+ Mobilizing Agents Using Indo-1-Loaded Hepatocytes

    • Anton M. Bennett, Oliver P. Flint, Gary M. Williams

    Pages 143-151

19. Confocal Fluorescence Ratio Imaging of pH in Plant Cells

    • M. D. Fricker, M. R. Blatt, N. S. White

    Pages 153-163

20. Analyses of the Calcium and Phosphorus Distribution in Predentine Tissue of Rat Incisors

    • H. P. Wiesmann, U. Plate, H. J. Höhling, R. H. Barckhaus

    Pages 165-171

Individual cells behave in surpnsmg ways that cannot be deduced from the averaged results of an organ as assessed by the use of conventional biochemical methods. Thus multicellular plant and animals systems are being investigated by an increasing array of histochemical and cytochemical techniques based on general chemical or specific immunological interactions to identify structural materials and to assess biological activities. In recent years there has been an increasing range of fluorescent probes, along with advanced computerised imaging and analysis techniques, which allows the behaviour of individual living cells to be followed in considerable detail. The parallel use of microinjection, microelectrodes and patch-clamping provides additional information about cells and their responses. Recombinant DNA technology has highlighted the desirability and the power of microinjecting defined materials into specific cells and so manipulating their fundamental biochemistry. New hypotheses are being tested which will form the cornerstone of future developments across the whole spectrum of biotechnology. The First European Workshop on Biotechnology Applications of Microinjection, Microscopic Imaging and Fluorescence was run at the University of East London, U.K, 21st-24th April, 1992 with the objective of bringing together a diverse group of individuals who were using these state-of-the-art applications for biotechnological exploration. A novel feature of the meeting was paiticipation by instrument manufacturers in the programme: there were hands-on workshops (where living cells could be examined), combined with the poster sessions.

• DNA
• Embryo
• HIV
• Mammalia
• biochemistry
• biotechnology
• chemistry
• fluorescence
• protein

• University of East London, London, UK

  Peter H. Bach, C. Hugh Reynolds, Jessica M. Clark, John Mottley, Phil L. Poole

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