Identification of Transcribed Sequences

1. Front Matter

   Pages i-xi

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2. Introduction: Seven Blind Men and an Elephant

   1. Introduction: Seven Blind Men and an Elephant

      • Miles B. Brennan

      Pages 1-4

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3. From Genomic DNA to Transcribed Sequences

   1. Classical Approaches

      1. Identification of Genes and Construction of a Transcriptional Map in Xq28

         • C. Tribioli, E. Maestrini, S. Bione, F. Tamanini, M. Mancini, C. Sala et al.

         Pages 5-10

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      2. Use of cDNA Selection and Evolutionarily Conserved Sequences to Isolate Transcribed Sequences from Region Xp11.21

         • Eric N. Burright, N. German Pasteris, Michael D. Bialecki, Jerome L. Gorski

         Pages 11-21

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      3. Identification of cDNAs by Direct Hybridization Using Cosmid Probes

         • Gregory G. Lennon, Kimberly Lieuallen

         Pages 23-28

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   2. Hybridization Based Approaches

      1. Locus Specific Identification of Transcribed Sequences Using Yacs and Whole Yeast Genomic DNA

         • Sankhavaram R. Patanjali, Hong Xia Xu, Satish Parimoo, Sherman M. Weissman

         Pages 29-35

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      2. Towards a Transcriptional Map of Human Chromosome 21

         • K. Gardiner, H. Xu, W. Bonds, F. Tassone, S. Parimoo, R. Sivakamasundari et al.

         Pages 37-49

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      3. Isolation of Expressed Sequences from the Chromosome 17q21 BRCA1 Region by Magnetic Bead Capture

         • Fergus J. Couch, Barbara L. Weber, Francis S. Collins, Danilo A. Tagle

         Pages 51-63

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      4. Towards a Transcriptional Map of the q21-q22 Region of Chromosome 7

         • J. M. Rommens, L. Mar, J. McArthur, L.-C. Tsui, S. W. Scherer

         Pages 65-79

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      5. Direct cDNA Selection Using Human and Mouse cDNAs: Application to Xq13.3 Chromosomal Region

         • J. Gecz, L. Villard, A. M. Lossi, M. Fontes

         Pages 81-90

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      6. A Sandwich-Hybridization Method for Specific and Efficient Selection of cDNA Clones from Genomic Regions

         • Denise Yan, Anand Swaroop

         Pages 91-99

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      7. Novel Strategy for Isolating Unknown Coding Sequences from Genomic DNA by Generating Genomic-cDNA Chimeras

         • Pudur Jagadeeswaran, Michael W. Odom, Edward J. Boland

         Pages 101-109

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      8. Identifying and Directly Purifying Transcribed Elements by Coincident Sequence Cloning

         • A. J. Brookes

         Pages 111-121

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      9. Finding Candidate Genes by Preparative in Situ Hybridization

         • J. C. Hozier, L. M. Davis, P. D. Siebert, K. Dietrich, M. C. Paterson

         Pages 123-138

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      10. Direct cDNA Screening of Genomic Reference Libraries - A Rapid Method for the Identification of Transcribed Sequences in Large Genomic Regions

          • Wolfgang Schwabe, Brenda J. Lawrence, Adelaide S. Robb, Rene M. Hopfinger, Ute Hochgeschwender, Miles B. Brennan

          Pages 139-155

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      11. Identification of Expressed Sequences on Human Chromosome 9q32–34

          • Jeffrey D. Falk, Hiroshi Usui, J. Gregor Sutcliffe

          Pages 157-167

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   3. Exon Trapping

      1. An Exon Trapping System Providing Size Selection of Spliced Clones and Facilitating Direct Cloning

         • Nicole A. Datson, Geoffrey M. Duyk, Lau A. J. Blonden, Gert-Jan B. Van Ommen, Johan T. Den Dunnen

         Pages 169-181

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      2. Isolation of Gene Sequences from the BRCA1 Region of Chromosome 17q21 by Exon Amplification

         • Kenneth J. Abel, Lucio H. Castilla, Alan J. Buckler, Fergus J. Couch, Peggy Ho, Ida Schaefer et al.

         Pages 183-198

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      3. Isolation of Coding Sequence from Cosmids and YACs by Exon Amplification

         • Michael North, Fernando Gibson, Stephen Brown, Beatrice Griffiths, Ellen Solomon, Hans Lehrach

         Pages 199-212

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      4. Integrated Transcriptional Maps of Large DNA Regions: Towards a Transcriptional Map of Human Chromosome 21

         • Marie-Laure Yaspo, Philippe Sanséau, Dean Nizetic, Bernhard Korn, Annemarie Poustka, Hans Lehrach

         Pages 213-228

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The Human Genome Project, an endeavor to map and sequence the entire human genome, has been in existence for almost seven years. One result of this effort has been the development of increasingly detailed genetic and physical maps spanning large regions of virtually every chromosome. Paralleling this has been the increasingly high resolution mapping of many &wnetic diseases. Together, these developments have facilitated the isolation of specific disease genes and are now motivating the construction of comprehensive transcriptional maps. This latter endeavor represents a new facet of the genome project, and as such requires the recognition and solution of a new set of problems, with the attendant development and application of a new set of techniques. The First International Workshop on the Identification of Transcribed Sequences in the Human Genome was held in 1991 and was attended by 23 investigators. Discussions at this meeting were largely devoted to defining the magnitude of the problem and describing the available techniques. A small number of laboratories reported the development of new techniques (at that time, for example, exon trapping, cDNA hybrid selection, direct cDNA screening, use of splice junction conserved sequences,etc.), but data were too limited to permit comparisons of their relative efficiencies.

• Chromosom
• DNA
• YAC
• cloning
• genes
• hybridization
• transcription

• National Institute of Mental Health, Bethesda, USA

  Ute Hochgeschwender

• Eleanor Roosevelt Institute for Cancer Research, Denver, USA

  Katheleen Gardiner

Policies and ethics