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Nanoscale Imaging of Synapses

New Concepts and Opportunities

  • Book
  • © 2014

Overview

Editors:
  1. U. Valentin Nägerl

    1. Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, France

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  2. Antoine Triller

    1. Institute of Biology at Ecole Normale Supérieure, Ecole Normale Supérieure, Paris, France

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    You can also search for this editor in PubMed   Google Scholar

  • Timeliness – Superresolution methods are beginning to transform neurobiological research
  • Relevance – Progress in synapse research hinges on advances in superresolution imaging
  • Competence – Chapters are written by leading innovators and practitioners in the field of bioimaging
  • Includes supplementary material: sn.pub/extras

Part of the book series: Neuromethods (NM, volume 84)

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Table of contents (11 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Nanoscale Imaging of Protein Molecules at the Postsynaptic Density

    • Xiaobing Chen, Christine Winters, Rita Azzam, Alioscka A. Sousa, Richard D. Leapman, Thomas S. Reese
    Pages 1-21

  3. Electron Tomography for the Study of Synaptic Ultrastructure in Fixed Brain Sections

    • Alain C. Burette, Richard J. Weinberg
    Pages 23-41

  4. Flash-and-Freeze Electron Microscopy: Coupling Optogenetics with High-Pressure Freezing

    • Shigeki Watanabe, M. Wayne Davis, Erik M. Jorgensen
    Pages 43-57

  5. Investigating AMPA Receptor Diffusion and Nanoscale Organization at Synapses with High-Density Single-Molecule Tracking Methods

    • Audrey Constals, Eric Hosy, Daniel Choquet
    Pages 59-74

  6. Visualizing the Ultrastructures and Dynamics of Synapses by Single-Molecule Nanoscopy

    • Christian G. Specht, Ignacio Izeddin, Maxime Dahan
    Pages 75-91

  7. Live-Cell PALM of Intracellular Proteins in Neurons

    • Nicholas A. Frost, Harold D. MacGillavry, Hsiangmin E. Lu, Thomas A. Blanpied
    Pages 93-123

  8. Monitoring Nanoscale Mobility of Small Molecules in Organized Brain Tissue with Time-Resolved Fluorescence Anisotropy Imaging

    • Kaiyu Zheng, James A. Levitt, Klaus Suhling, Dmitri A. Rusakov
    Pages 125-143

  9. Imaging Signaling Transduction in Single Dendritic Spines

    • Nathan Hedrick, Ryohei Yasuda
    Pages 145-159

  10. Imaging-Based Measures of Synaptic Tenacity

    • Noam E. Ziv
    Pages 161-185

  11. Zooming in on the (Peri)synaptic Extracellular Matrix

    • Svetlana Korotchenko, Francesca Cella Zanacchi, Alberto Diaspro, Alexander Dityatev
    Pages 187-203

  12. Two-Photon Excitation STED Microscopy for Imaging Synapses and Glia in Acute Brain Slices

    • Philipp Bethge, U. Valentin Nägerl
    Pages 205-220

  13. Back Matter

    Pages 221-231
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Keywords

About this book

Synapses underlie rapid and flexible neural communication in the brain and they hold the key to understanding higher brain functions in health and disease. Because they are very small and highly dynamic, it is very difficult to study them with traditional techniques. Fortunately, recent ground-breaking advances in optical microscopy (e.g. STED, PALM, STORM, SIM) have greatly improved our ability to image living synapses at the nanoscale, even down to the level of single molecules. The proposed volume brings together leading researchers to review these exciting new techniques and their application in neurobiological research. It will explain and discuss the basic principles behind the various superresolution modalities, how they are implemented, what their scope and limitations are etc. In addition, several key research discoveries on synapses enabled by these novel approaches will be highlighted.

Editors and Affiliations

  • Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, France

    U. Valentin Nägerl

  • Institute of Biology at Ecole Normale Supérieure, Ecole Normale Supérieure, Paris, France

    Antoine Triller

Bibliographic Information

  • Book Title: Nanoscale Imaging of Synapses

  • Book Subtitle: New Concepts and Opportunities

  • Editors: U. Valentin Nägerl, Antoine Triller

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4614-9179-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4614-9178-1Published: 05 February 2014

  • Softcover ISBN: 978-1-4939-4918-2Published: 23 August 2016

  • eBook ISBN: 978-1-4614-9179-8Published: 04 February 2014

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: X, 231

  • Number of Illustrations: 8 b/w illustrations, 49 illustrations in colour

  • Topics: Neurosciences, Neurobiology

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