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Metastasis Research Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Miriam Dwek

    1. Molecular and Applied Biosciences, University of Westminster School of Life Sciences, London, United Kingdom

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  2. Udo Schumacher

    1. Morphologie, Universitätsklinikum Hamburg Inst. Anatomie und Experimentelle, Hamburg, Germany

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    You can also search for this editor in PubMed   Google Scholar

  3. Susan A. Brooks

    1. Oxford Brookes University School of Life Sciences, Oxford, United Kingdom

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    You can also search for this editor in PubMed   Google Scholar

  • Presents current up-to-date methodologies
  • Features tips on trouble shooting and avoiding known pitfalls
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1070)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
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  2. In Vitro Invasion Assay Using Matrigel™: A Reconstituted Basement Membrane Preparation

    • Debbie M. S. Hall, Susan A. Brooks
    Pages 1-11

  3. Single Cell and Spheroid Collagen Type I Invasion Assay

    • Olivier De Wever, An Hendrix, Astrid De Boeck, Frank Eertmans, Wendy Westbroek, Geert Braems et al.
    Pages 13-35

  4. Rocking Adhesion Assay System to Study Adhesion and Transendothelial Migration of Cancer Cells

    • Deepashree Bapu, Munira Khadim, Susan A. Brooks
    Pages 37-45

  5. Small-Cell Lung Cancer (SCLC) Cell Adhesion on E- and P-Selectin Under Physiological Flow Conditions

    • Ulrich Richter
    Pages 47-56

  6. Adhesion of Tumor Cells to Matrices and Endothelium

    • Clara M. Yates, Helen M. McGettrick, Gerard B. Nash, G. Ed Rainger
    Pages 57-75

  7. Cell Aggregation Assays

    • Delphine Debruyne, Tom Boterberg, Marc E. Bracke
    Pages 77-92

  8. Chick Heart Invasion Assay

    • Marc E. Bracke, Virinder S. Parmar, Anthony L. DePass, Christian V. Stevens, Barbara W. Vanhoecke, Marc M. Mareel
    Pages 93-106

  9. Computer Simulation of the Metastatic Progression

    • Gero Wedemann, Anja Bethge, Volker Haustein, Udo Schumacher
    Pages 107-116

  10. Theoretical Considerations in Using Animal Models of Metastasis and Brief Methodology for In Vivo Colorectal Cancer Models in SCID and Nude Mice

    • Sue A. Watson, Rajendra Kumari
    Pages 117-129

  11. Syngeneic Murine Metastasis Models: B16 Melanoma

    • Raffaella Giavazzi, Alessandra Decio
    Pages 131-140

  12. Imageable Clinically Relevant Mouse Models of Metastasis

    • Robert M. Hoffman
    Pages 141-170

  13. Imaging Metastatic Cell Trafficking at the Cellular Level In Vivo with Fluorescent Proteins

    • Robert M. Hoffman
    Pages 171-179

  14. Ultrasound Techniques for the Detection of Tumors and Metastases in Small Animals

    • Michael Didié, Wolfram-Hubertus Zimmermann
    Pages 181-190

  15. The PFP/RAG2 Double-Knockout Mouse in Metastasis Research: Small-Cell Lung Cancer and Prostate Cancer

    • Imke Müller, Sebastian Ullrich
    Pages 191-201

  16. Ultrasound-Guided Intracardial Injection and In Vivo Magnetic Resonance Imaging of Single Cells in Mice as a Paradigm for Hematogenous Metastases

    • Johannes Salamon, Kersten Peldschus
    Pages 203-211

  17. Magnetic Resonance Imaging of Metastases in Xenograft Mouse Models of Cancer

    • Kersten Peldschus, Harald Ittrich
    Pages 213-222

  18. Spontaneous and Experimental Metastasis Models: Nude Mice

    • Janet E. Price
    Pages 223-233

  19. Identifying the Origin and Phenotype of Cells in Tumor Xenografts

    • Rosemary Jeffery, Pooja Seedhar, Richard Poulsom
    Pages 235-245

  20. Erratum

    • Deepashree Bapu, Munira Khadim, Susan A. Brooks
    Pages E1-E1
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Keywords

About this book

Diverse molecular, cellular, and environmental events must all come together to allow the successful formation of secondary cancers, metastases.  The second edition of Metastasis Research Protocols, brings together updated versions of the seminal technique that were presented in the first edition and also includes new techniques that have recently been shown to be important in illuminating the processes underlying this important area of biology. Volume 2 presents techniques applicable at the level of living cells and tissues, and presents methodologies applicable to cell behaviour in vitro, in animal models and in mathematical constructs. The aim is the study of  the interaction between cancer cells and their host/environment. The focus throughout is on the tools that have been shown to be helpful in unravelling the processes important in cancer metastasis.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Metastasis Research Protocols, Second Edition seeks to aid scientists in the further study of new methods in the area of metastasis research.

Editors and Affiliations

  • Molecular and Applied Biosciences, University of Westminster School of Life Sciences, London, United Kingdom

    Miriam Dwek

  • Morphologie, Universitätsklinikum Hamburg Inst. Anatomie und Experimentelle, Hamburg, Germany

    Udo Schumacher

  • Oxford Brookes University School of Life Sciences, Oxford, United Kingdom

    Susan A. Brooks

Bibliographic Information

  • Book Title: Metastasis Research Protocols

  • Editors: Miriam Dwek, Udo Schumacher, Susan A. Brooks

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4614-8244-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2014

  • Hardcover ISBN: 978-1-4614-8243-7Published: 04 October 2013

  • Softcover ISBN: 978-1-4939-4887-1Published: 27 August 2016

  • eBook ISBN: 978-1-4614-8244-4Published: 04 October 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 250

  • Number of Illustrations: 24 b/w illustrations, 10 illustrations in colour

  • Topics: Cancer Research, Oncology, Cell Culture

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