Laboratory Protocols in Fungal Biology

1. Front Matter

   Pages i-xxv

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2. Safety Norms and Regulations in Handling Fungal Specimens

   • Finola E. Cliffe

   Pages 1-7

3. Methods of Cryopreservation in Fungi

   • Ladislav Homolka

   Pages 9-16

4. Long-Term Preservation of Fungal Cultures in All-Russian Collection of Microorganisms (VKM): Protocols and Results

   • Svetlana M. Ozerskaya, Natalya E. Ivanushkina, Galina A. Kochkina, Svetlana S. Eremina, Alexander N. Vasilenko, Nadezhda I. Chigineva

   Pages 17-65

5. Fungal Specimen Collection and Processing

   • Anthonia O’Donovan, Vijai Kumar Gupta, Maria G. Tuohy

   Pages 67-72

6. Chemical and Molecular Methods for Detection of Toxigenic Fungi and Their Mycotoxins from Major Food Crops

   • S. Chandra Nayaka, M. Venkata Ramana, A. C. Udayashankar, S. R. Niranjana, C. N. Mortensen, H. S. Prakash

   Pages 73-90

7. Identification Key for the Major Growth Forms of Lichenized Fungi

   • Jeyabalan Sangeetha, Devarajan Thangadurai

   Pages 91-112

8. Microscopic Methods for Analytical Studies of Fungi

   • De-Wei Li

   Pages 113-131

9. Scanning Electron Microscopy for Fungal Sample Examination

   • Eduardo Alves, Gilvaine Ciavareli Lucas, Edson Ampélio Pozza, Marcelo de Carvalho Alves

   Pages 133-150

10. High-Resolution Imaging and Force Spectroscopy of Fungal Hyphal Cells by Atomic Force Microscopy

    • Biplab C. Paul, Hui Ma, Laelie A. Snook, Tanya E. S. Dahms

    Pages 151-160

11. Use of Fourier-Transform Infrared (FTIR) Microscopy Method for Detection of Phyto-Fungal Pathogens

    • Vitaly Erukhimovitch, Mahmoud Huleihel

    Pages 161-167

12. Diagnosis of Parasitic Fungi in the Plankton: Technique for Identifying and Counting Infective Chytrids Using Epifluorescence Microscopy

    • Télesphore Sime-Ngando, Serena Rasconi, Mélanie Gerphagnon

    Pages 169-174

13. Fungal Cell Wall Analysis

    • Pilar Pérez, Juan C. Ribas

    Pages 175-196

14. Histopathological Technique for Detection of Fungal Infections in Plants

    • Vijai Kumar Gupta, Brejesh Kumar Pandey

    Pages 197-200

15. Development of Media for Growth and Enumeration of Fungi from Water

    • Segula Masaphy

    Pages 201-209

16. Sabouraud Agar for Fungal Growth

    • Janelle M. Hare

    Pages 211-216

17. A Method for the Formation of Candida Biofilms in 96 Well Microtiter Plates and Its Application to Antifungal Susceptibility Testing

    • Christopher G. Pierce, Priya Uppuluri, Jose L. Lopez-Ribot

    Pages 217-223

18. Screening for Compounds Exerting Antifungal Activities

    • Jean-Paul Ouedraogo, Ellen L. Lagendijk, Cees A. M. J. J. van den Hondel, Arthur F. J. Ram, Vera Meyer

    Pages 225-230

19. Fluorescence In Situ Hybridization of Uncultured Zoosporic Fungi

    • Télesphore Sime-Ngando, Marlène Jobard, Serena Rasconi

    Pages 231-236

20. Staining Techniques and Biochemical Methods for the Identification of Fungi

    • Jeyabalan Sangeetha, Devarajan Thangadurai

    Pages 237-257

Laboratory Protocols in Fungal Biology presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently used methods in fungal biology. Almost all important methods available in the area of fungal biology viz. taxonomic keys in fungi; histopathological and microscopy techniques; proteomics methods; genomics methods; industrial applications and related techniques; and bioinformatics tools in fungi are covered and complied in one book. Chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting. Each chapter is self-contained and written in a style that enablesthe reader to progress from elementary concepts to advanced research techniques.  Laboratory Protocols in Fungal Biology is a valuable tool for both beginner research workers and experienced professionals.

Coming Soon in the Fungal Biology series:

 

Goyal, Manoharachary / Future Challenges in Crop Protection Against Fungal Pathogens

Martín, García-Estrada, Zeilinger / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites

Zeilinger, Martín, García-Estrada / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites, Volume 2

van den Berg, Maruthachalam / Genetic Transformation Systems in Fungi

Schmoll, Dattenbock / Gene Expression Systems in Fungi

Dahms / Advanced Microscopy in Mycology

• Fungal Biology
• Mycology
• Plant Sciences

From the book reviews:

“The book ‘Laboratory Protocols in Fungal Biology’ written by international researchers, offers diverse modern laboratory techniques in the field of fungal biology. … Each chapter is comprehensive, provides readily reproducible protocols and is easy to grasp by beginners. This book is a valuable guide to budding mycologists as well as professionals. It is recommended to all the Universities. A crucial Laboratory Manual for Fungal Biologists, Taxonomists, Fungal Biotechnologists, Biochemists, Histochemists, Plant Pathologists and Laboratory technicians.” (Melvina D’souza and Samantha C. Karunarathna, Fungal Diversity, December, 2014)

“This new book is very much hands-on, and covers an extraordinarily diverse range of topics. … Chapters are well-referenced to primary literature, and there are numerous links to website. There are numerous illustrations, most clearly presented, and in some cases using colour. This is a reference work that deserves to be accessible in all mycology laboratories … .” (IMA Fungus, Vol. 4 (1), 2013)

• , Department of Biochemistry, National University of Ireland Galway, Galway, Ireland

  Vijai Kumar Gupta, Maria G. Tuohy, Manimaran Ayyachamy

• , Manufacturing Sciences and Technology, Pfizer Ireland Pharmaceuticals, Clondalkin, Ireland

  Kevin M. Turner

• , School of Natural Sciences, National University of Ireland Galway, Galway, Ireland

  Anthonia O’Donovan

Editors

Vijai Kumar Gupta is Assistant Professor of Biotechnology at MITS University, India. Presently, he is working as a Post Doctoral Research Scientist, at NUIG, Ireland. He obtained his Ph.D. in Microbiology from Dr. R.M.L. Avadh University, India. He has been honoured with several awards, including the prestigious Indian ICAR Senior Research Fellowship and Young Scientist Award-2009, 2011. He has over seven years of research experience in Applied Microbiology and Biotechnology with a special interest in fungal biology.  He has edited books published from Lambert Academic Publishing, Germany; Nova Science Publishers, USA; Science Publisher, CRC Press, Taylor & Francis, USA; and Springer, USA.

Dr. Maria G. Tuohy is the Head of the Molecular Glycobiotechnology Research Group, NUI Galway. She has more than 20 years experience in the molecular biochemistry, genetics and biotechnology of fungi, with a special interest in thermophilic ascomycetes and the characterization of these fungi as cell factories for protein production, including novel thermostable enzymes/enzyme systems. Dr. Tuohy has authored 132 research publications. She is also a reviewer for international journals and funding agencies, an associate editor for Microbiology and co-editor of four books with Dr. V. K. Gupta.

Associate Editors

Dr. Manimaran Ayyachamy is a Research Scientist with NUIG, Ireland. He obtained his PhD in Microbiology from the University of Madras, India. He has twelve years of research experience in microbial biotechnology with a special interest in microbial enzymes and biofuels technology. He has co-edited books published from Lambert Academic Publishing, Germany; Nova Science Publishers, USA; Science Publisher, CRC Press, Taylor & Francis, USA; and Springer, USA with Dr. V.K. Gupta

Dr. Kevin M. Turner is a Research Scientist in the division of Manufacturing Sciences and Technology in Pfizer Ireland Pharmaceuticals, Dublin. He obtained his PhD from the University of Limerick, Ireland. He has three years of research experience in microbial biotechnology.

Dr. Anthonia O’Donovan is a Postdoctoral Research Scientist with NUIG, Ireland. She obtained her PhD in Biochemistry from the National University of Ireland Galway, Ireland. She has seven years of research experience in microbial enzymes and biofuels technology.

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