Multidimensional Microscopy

1. Front Matter

   Pages i-xvii

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2. Confocal Microscopy: Basic Principles and System Performance

   • Colin J. R. Sheppard

   Pages 1-31

3. Designing a Confocal Fluorescence Microscope

   • Ernst H. K. Stelzer

   Pages 33-51

4. Theory and Principal Technologies of the Laser Scanning Confocal Microscope

   • Hisao Kitagawa

   Pages 53-71

5. Simultaneous Ultraviolet and Visible Excitation Confocal Microscopy

   • Michael Burke, David Clapham

   Pages 73-85

6. High Resolution Confocal Microscopy of Phase and Amplitude Objects

   • Carol J. Cogswell

   Pages 87-102

7. Interferometric Microscopy — An Overview of the Optical Characterization of Inertial-fusion Targets

   • Hyo-gun Kim, Mark D. Wittman

   Pages 103-121

8. X-Ray Microscopy — Possibilities for Three Dimensional Imaging

   • Alan Michette, Richard Cave

   Pages 123-132

9. High Resolution Projection X-Ray Microscopy

   • Keiji Yada, Shoichi Takahash

   Pages 133-150

10. Cone-beam X-ray Microtomography

    • Ge Wang, Tein-Hsiang Lin, Douglus M. Shinozaki, Hyo-gun Kim, Ping-chin Cheng

    Pages 151-169

11. High Resolution Low Voltage Scanning Electron Microscopy: Reduced Radiation Damage on Cryo-specimens

    • Ya Chen, James B. Pawley

    Pages 171-190

12. Image Restoration in Light Microscopy

    • Ge Wang, Wen-shan Liou, Tein-Hsiang Lin, Ping-chin Cheng

    Pages 191-208

13. Image Quality Considerations in Computerized 2-D and 3-D Microscopy

    • Andres Kriete

    Pages 209-230

14. Analysis and Presentation of Three Dimensional Data Sets

    • Jagath K. Samarabandu, Raj Acharya, Ping-chin Cheng

    Pages 231-250

15. The Processing of Three-dimensional Confocal Data Sets

    • Eric S. Boxall, Nick S. White, Gerald S. Benham

    Pages 251-266

16. Effective Laser Lines for Biological Confocal Microscopy

    • Anna Smallcombe, Gerald S. Benham

    Pages 267-289

17. Visualizing DNA Replication in Three Dimensions

    • Ronald Berezney, Chunling Meng, Jagath K. Samarabandu, Raj Acharya, Tein-Hsiang Lin, Ping-chin Cheng

    Pages 291-303

18. Study of Nuclear Proteins Associated with Cell Proliferation, Differentiation, and Mitosis by Confocal Fluorescence Microscopy

    • Tang K. Tang, Chieh-Ju C. Tang, Tsui-Chun Tsou, Tse-Ming Hong, Ruey-Long Hong, Cheng-Ju Chang et al.

    Pages 305-319

19. Two- and Three-Dimensional Confocal Microscopy of the Eye

    • Barry R. Masters

    Pages 321-338

20. Confocal Microscopy of Botanical Specimens

    • Ping-chin Cheng, Dayakar R. Pareddy, Tein-Hsiang Lin, Jagath K. Samarabandu, Raj Acharya, Ge Wang et al.

    Pages 339-380

Modern cell biology is being revolutionized by the wedding of microscopy and computers. This book describes the new instrumentation and methods which allow three-dimensional reconstruction of specimens. Multidimensional Microscopy will be of interest to cell biologists, microscopists, and basic biomedical researchers whose work involves microscopic techniques. This book presents current results on a very active field in modern biology: methods in light and electron microscopy that allow the reconstruction of three-dimensional objects with the aid of computers. The book emphasizes the methods that can be used and examples of biological systems to which they have been applied. It includes extensive descriptions of confocal microscopy and its applications, as well as chapters on X-ray microscopy, low-voltage electron microscopy, and image reconstruction. This is an impressive summary of state-of-the-art methods in microscopy, in which microscopes and computers are being joined to permit specimens to be examined and reconstructed in three dimensions. Will be of interest to cell biologists, biomedical researchers, and microscopists.

• X-ray
• biology
• calculus
• cell biology
• computer
• electron microscopy
• fluorescence
• imaging
• microscopy
• quality
• tomography

• Advanced Microscopy and Imaging Lab Dept. of Electrical and Computer Engineering, State University of New York at Buffalo, Buffalo, USA

  P. C. Cheng, T. H. Lin

• Institute of Zoology, Academia Sinica, Taipei, Taiwan, Republic of China

  W. L. Wu, J. L. Wu

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