Triple Helix Forming Oligonucleotides

1. Front Matter

   Pages i-xv

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2. Background and Structural Aspects

   1. Front Matter

      Pages 1-1

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   2. The Antigene Strategy: Progress and Perspectives in Selective Gene Silencing

      • Claude Hélène

      Pages 3-16

   3. Triple-Helix Structure. The Triple-Helix-Forming Oligonucleotide

      • Horea Porumb

      Pages 17-31

   4. Thermodynamic State Diagrams of Oligonucleotide Triple Helices

      • G. Eric Plum, K. J. Breslauer

      Pages 33-47

   5. Imaging of Triplexes by Electron and Scanning Force Microscopy

      • Dmitry Cherny

      Pages 49-62

   6. Chemical Modifications of Triple Helix Forming Oligonucleotides

      • Ulysse Asseline

      Pages 63-73

   7. Triple Helix Formation with Modified Oligonucleotides

      • Sergei M. Gryaznov

      Pages 75-85

   8. Oligonucleotides as Artificial DNases

      • Valentina Zarytova, Asya Levina

      Pages 87-97

3. The Biology of Triple Helices

   1. Front Matter

      Pages 99-99

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   2. Potential Mechanisms of Action

      • L. James Maher

      Pages 101-115

   3. Oligonucleotide Uptake and Delivery in Tissue Culture Cells

      • Scot W. Ebbinghaus, Nadarajah Vigneswaran, Charles M. Mayfield, David T. Curiel, Donald M. Miller

      Pages 117-127

   4. Triple-Stranded Complexes as Antigene Molecules: Transcription Inhibition Ex Vivo

      • Carine Giovannangeli

      Pages 129-143

   5. Gene-Targeting Triple Helix Forming Purine Oligonucleotides

      • Fedor Svinarchuk, Claude Malvy

      Pages 145-157

   6. DNA Triple Helix as a Tool to Regulate Cytokine Gene Expression

      • Marina Kochetkova, Mary Frances Shannon

      Pages 159-166

   7. Genome Modification by Triplex-Forming Oligonucleotides

      • Karen M. Vasquez, Peter M. Glazer

      Pages 167-179

   8. Psoralen-Coupled Oligonucleotides: In Vivo Binding and Repair

      • François-Xavier Barre, Linda L. Pritchard, Annick Harel-Bellan

      Pages 181-192

   9. Structure and Biology of H DNA

      • Sergei M. Mirkin

      Pages 193-222

   10. Triplex-Binding Proteins

       • Anne-Laure Guieysse, Daniele Praseuth

       Pages 223-231

   11. RNA and Triple Helices

       • Kyonggeun Yoon, Jörg Jendis, Karin Moelling

       Pages 233-242

Sequence-specific DNA binding ligands, amongst which triple helix forming oligonucleotides are the most efficient as yet, represent promising tools in a number of fields. One of their most promising applications is as antiviral tools: they can specifically target a viral gene, even if it is integrated into the host genome, and be used to specifically inactivate the viral gene or even destroy the cells harboring this gene. However, from science fiction to science there remains a gap; and we are at the moment on the threshold of this fascinating field. Triple Helix Forming Oligonucleotides considers the different aspects of the design and improvement, current or future, of these molecules and their structural analysis, as well as their applications, with special emphasis on the attempts to obtain biological effects of these potentially important tools. What emerges is that the current state of the research is encouraging, and that these molecules are already useful in some biotechnology applications.

• DNA
• Nucleotide
• Purine
• RNA
• Vivo
• genes
• microscopy
• transcription

• CNRS, France

  Claude Malvy, Annick Harel-Bellan, Linda L. Pritchard

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