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Manual of Antisense Methodology

  • Book
  • © 1999

Overview

Editors:
  1. Gunther Hartmann

    1. University of Munich, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Stefan Endres

    1. University of Munich, Germany

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Perspectives in Antisense Science (DARE, volume 4)

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Table of contents (11 chapters)

  1. Front Matter

    Pages i-xiii
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  2. The Chemistry of Antisense Oligonucleotides

    1. Front Matter

      Pages 1-1
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    2. Chemical Synthesis and Purification of Phosphorothioate Antisense Oligonucleotides

      • Yogesh S. Sanghvi, Mark Andrade, Rantjit R. Deshmukh, Lars Holmberg, Anthony N. Scozzari, Douglas L. Cole
      Pages 3-23

    3. How to Choose Optimal Antisense Targets in an mRNA

      • Bernhard Schu, Heinrich Brinkmeier
      Pages 25-38

  3. Antisense Application in vitro

    1. Front Matter

      Pages 39-39
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    2. How to Characterize and Improve Oligonucleotide Uptake into Leukocytes

      • Martin Bidlingmaier, Anne Krug, Gunther Hartmann
      Pages 41-56

    3. Strategies for Targeted Uptake of Antisense Oligonucleotides in Hepatocytes

      • Wolf-Bernhard Offensperger, Christian Thoma, Darius Moradpour, Fritz von Weizsäcker, Hubert E. Blum, Jerzy Madon
      Pages 57-77

    4. How to Exclude Immunostimmulatory and Other Nonantisense Effects of Antisense Oligonucleotides

      • Arthur M. Krieg
      Pages 79-89

    5. Testing Antisense Oligonucleotides in Controlled Cell Culture Assays

      • W. Michael Flanagan
      Pages 91-106

    6. Inhibition of TNF Synthesis by Antisense Oligonucleotides

      • Lester Kobzik, Margaret Taylor
      Pages 107-123

    7. Inhibition of IGF-I Receptor Expression by Antisense Oligonucleotides in vitro and in vivo

      • Mariana Resnicoff
      Pages 125-141

  4. Antisense Application in vivo

    1. Front Matter

      Pages 143-143
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    2. How to Test Antisense Oligonucleotides in Animals

      • Rainer Spanagel, Christoph Probst, Deborah C. Mash, T. Skutella
      Pages 145-165

    3. Lipid-Based Carriers for the Systemic Delivery of Antisense Drugs

      • Murray S. Webb, Sandra K. Klimuk, Sean C. Semple, Michael J. Hope
      Pages 167-190

    4. Designing a Clinical Antisense Study

      • Finbarr E Cotter, Dean Fennell
      Pages 191-201

  5. Back Matter

    Pages 203-205
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Keywords

About this book

In the past few years, antisense methodology has moved from in vitro studies to in vivo studies and first human trials. While the basic concept of antisense technology is simple, the methodological problems associated with its use are numerous and complex. Antisense- based methods have proven to be a field of research where careful attention to experimental protocols and appropriate controls is necessary.
The Manual of Antisense Methodology emphasizes the application of antisense oligonucleotides, and is a guide for the identification of antisense and non-antisense effects in different experimental settings. The work is organized into three sections: antisense application in vitro, antisense application in vivo (animal models) and finally, clinical antisense studies. Where at all possible, the methods are described in sufficient detail to allow reproduction of a given experiment.
The Manual of Antisense Methodology will be of interest to researchers in immunology, cancer research, pharmacology and internal medicine; and physicians conducting clinical studies in these fields.

Editors and Affiliations

  • University of Munich, Germany

    Gunther Hartmann, Stefan Endres

About the editors

Gunther Hartmann, M.D. is a clinical and research fellow at the Division of Clinical Pharmacology, University of Munich. Stefan Endres, M.D. is an internist with subspecialties in gastroenterology and intensive care medicine. He is Chief of the Division of Clinical Pharmacology of the medical faculty at the University of Munich.

Bibliographic Information

  • Book Title: Manual of Antisense Methodology

  • Editors: Gunther Hartmann, Stefan Endres

  • Series Title: Perspectives in Antisense Science

  • DOI: https://doi.org/10.1007/978-1-4615-5067-9

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media New York 1999

  • Hardcover ISBN: 978-0-7923-8539-4Published: 31 May 1999

  • Softcover ISBN: 978-1-4613-7304-9Published: 12 October 2012

  • eBook ISBN: 978-1-4615-5067-9Published: 06 December 2012

  • Series ISSN: 1566-0389

  • Edition Number: 1

  • Number of Pages: XIII, 205

  • Topics: Biochemistry, general, Pharmacy, Human Genetics

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