Coronaviruses

1. Front Matter

   Pages i-xii

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2. Coronavirus Genes: Comparative Aspects

   1. Front Matter

      Pages 1-1

      PDF

   2. Sequence Analysis of CCV and its Relationship to FIPV, TGEV and PRCV

      • Brian C. Horsburgh, T. David K. Brown

      Pages 3-9

   3. Genomic Organization and Expression of the 3’ End of the Canine and Feline Enteric Coronaviruses

      • H. Vennema, J. W. A. Rossen, J. Wesseling, M. C. Horzinek, P. J. M. Rottier

      Pages 11-16

   4. Cloning and Sequence Analysis of the Spike Gene from Several Feline Coronaviruses

      • A. Paul Reed, Sharon Klepfer, Timothy Miller, Elaine Jones

      Pages 17-21

   5. Genomic Organisation of a Virulent Taiwanese Strain of Transmissible Gastroenteritis Virus

      • C.-M. Chen, D. H. Pocock, P. Britton

      Pages 23-28

   6. The Use of PCR Genome Mapping for the Characterisation of TGEV Strains

      • P. Britton, S. Kottier, C.-M. Chen, D. H. Pocock, H. Salmon, J. M. Aynaud

      Pages 29-34

   7. Evolution and Tropism of Transmissible Gastroenteritis Coronavirus

      • Luis Enjuanes, Carlos Sánchez, Fátima Gebauer, Ana Méndez, Joaquín Dopazo, María L. Ballesteros

      Pages 35-42

   8. Transmissible Gastroenteritis Virus and Porcine Respiratory Coronavirus: Molecular Characterization of the S Gene Using cDNA Probes and Nucleotide Sequence Analysis

      • Daral J. Jackwood, Insoo Bae, Renee J. Jackwood, Linda J. Saif

      Pages 43-48

   9. Sequence Analysis of the Nucleocapsid Protein Gene of Porcine Epidemic Diarrhoea Virus

      • Kurt Tobler, Anne Bridgen, Mathias Ackermann

      Pages 49-54

   10. Genome Organization of Porcine Epidemic Diarrhoea Virus

       • M. Duarte, J. Gelfi, P. Lambert, D. Rasschaert, H. Laude

       Pages 55-60

   11. Characterization of the Nonstructural and Spike Proteins of the Human Respiratory Coronavirus OC43: Comparison with Bovine Enteric Coronavirus

       • Samir Mounir, Patrick Labonté, Pierre J. Talbot

       Pages 61-67

   12. Identification, Expression in E. coli and Insect Cells of the Non-Structural Protein NS2 Encoded by mRNA₂ of Bovine Coronavirus (BCV)

       • P. Boireau, M. F. Madelaine, D. Saulnier, J. Laporte, J. F. Vautherot

       Pages 69-74

   13. Characterization of the Human Coronavirus 229E (HCV 229E) Gene 1

       • Jens Herold, Thomas Raabe, Stuart G. Siddell

       Pages 75-79

   14. Identification of Coronaviral Conserved Sequences and Application to Viral Genome Amplification

       • Anne Bridgen, Kurt Tobler, Mathias Ackermann

       Pages 81-82

3. Transcription, Replication and Genome Engineering

   1. Front Matter

      Pages 83-83

      PDF

   2. Studies into the Mechanism for MHV Transcription

      • Mary C. Schaad, Wan Chen, Sheila A. Peel, Ralph S. Baric

      Pages 85-90

   3. Analysis of the CIS-Acting Elements of Coronavirus Transcription

      • Myungsoo Joo, Shinji Makino

      Pages 91-97

   4. Control of TGEV mRNA Transcription

      • Julian Hiscox, David H. Pocock, Paul Britton

      Pages 99-104

   5. An Intraleader Open Reading Frame is Selected from a Hypervariable 5’ Terminus during Persistent Infection by the Bovine Coronavirus

      • Martin A. Hofmann, Savithra D. Senanayake, David A. Brian

      Pages 105-109

Coronaviruses represent a major group of viruses of both molecular biological interest and clinical significance in animals and humans. During the past two decades, coronavirus research has been an expanding field and, since 1980, an international symposium was held every 3 years. We organized the yth symposium for providing an opportunity to assess important progresses made since the last symposium in Cambridge (U. K. ) and to suggest areas for future investigations. The symposium, held in September 1992, in Chantilly, France, was attended by 120 participants representing the majOlity of the laboratories engaged in the field. The present volume collects 75 papers which were presented during the yth symposium, thus providing a comprehensive view of the state of the art ofCoronavirology. The book is divided into 7 chapters. The first chapters gather reports dealing with genome organization, gene expression and structure-function relationships of the viral polypeptides. New sequence data about as yet poorly studied coronaviruses - canine coronavirus CCY and porcine epidemic diarrhoea virus PEDY - are presented. Increasing efforts appear to be devoted to the characterization of products of unknown function, encoded by various open reading frames present in the coronavirus genomes or delived from the processing of the large polymerase polyprotein. Due to the extreme size of their genome, the genetic engineering ofcoronavi\'uses through the production of full length cDNA clones is presently viewed as an unachievahle task.

• Polypeptide
• gene expression
• genetic engineering
• molecular biology
• polymer
• protein
• virus

• Institut National de la Recherche Agronomique (INRA), Jouy-en-Josas, France

  Hubert Laude, Jean-François Vautherot

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