Research Methods in Neurochemistry

1. Front Matter

   Pages i-xx

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2. Properties of Intact Neural Tissues

   1. Front Matter

      Pages 1-1

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   2. Use of the Isolated Canine Brain in Studies of Cerebral Metabolism, Metabolite Transport, and Cerebrovascular Physiology

      • David D. Gilboe, A. Lorris Betz, Lester R. Drewes

      Pages 3-42

   3. Axoplasmic Transport

      • Stephen C. Bondy

      Pages 43-66

   4. Transport of Amino Acids in Brain Slices

      • S. S. Oja, M.-L. Vahvelainen

      Pages 67-137

   5. Glycine Enzymes and Uptake Systems

      • Marie Louise Uhr, Graham A. R. Johnston

      Pages 139-163

3. Components of Neural Tissues: Enzymes

   1. Front Matter

      Pages 165-165

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   2. Assay and Purification of Brain Monoamine Oxidase

      • Moussa B. H. Youdim

      Pages 167-207

   3. Acetylcholinesterase

      • Israel Silman, Yadin Dudai

      Pages 209-252

   4. Radiochemical Assays for Choline Acetyltransferase and Acetylcholinesterase

      • F. Fonnum

      Pages 253-275

   5. Phosphate-Activated Glutaminase in Brain

      • Elling Kvamme, Gerd Svenneby

      Pages 277-290

4. Components of Neural Tissues: Lipids, Proteins, and Polylipids

   1. Front Matter

      Pages 291-291

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   2. Analysis of Phospholipids by Sequential Chemical Degradation

      • A. Sheltawy

      Pages 293-307

   3. Analysis of Free and Esterified Fatty Acids in Neural Tissues Using Gradient-Thickness Thin-Layer Chromatography (GT-TLC)

      • Nicolás G. Bazán, Haydee E. P. Bazán

      Pages 309-324

   4. Methods for Studying Protein Phosphorylation in Cerebral Tissues

      • Richard Rodnight, Martin Reddington, Maria Gordon

      Pages 325-367

   5. Assays of Hypothalamic Releasing and Inhibiting Hormones

      • Akira Arimura, Andrew V. Schally

      Pages 369-392

   6. Structure-Activity Relationship of LH and FSH Releasing Hormone

      • David H. Coy, Esther J. Coy, Andrew V. Schally

      Pages 393-406

5. Components of Neural Tissues: Amino Acids

   1. Front Matter

      Pages 407-407

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   2. Assay Procedures for Polyamines and GABA in Animal Tissues with Special Reference to Dansylation Methods

      • Nikolaus Seiler

      Pages 409-441

   3. The Use of Dansyl-Chloride for the Detection of Amino Acids and Serotonin in Nervous Tissue

      • B. E. Leonard, N. N. Osborne

      Pages 443-462

With the continued rapid expansion of neurochemical research, there has been no shortage of new developments in methodology for this third volume of Research Methods in Neurochemistry. As in previous volumes we have again tried to provide some balance in the subjects represented. The wisdom of this policy may be questioned since it can lead to delay in publica­ tion, but there are many approaches to the chemical study of the nervous system and a methods book needs to stand on its own as well as be part of a series. In one respect, however, the present volume departs from this policy, in that we have included two chapters on micromethods for analyzing amines and amino acids, both giving special emphasis to dansylation techniques. These chapters are complementary and we feel justified in publishing them in one volume in view of the importance of such micromethods for the study of neural systems. At the other end of the scale, particular attention may be drawn to the chapter by D. D. Gilboe and colleagues describing their remarkable procedures for studying metabolism in the isolated canine brain. We were fortunate also in persuading S. S. Oja to extend the general prin­ ciples of transport systems he described in Volume 2 to amino acids in brain slices. In addition, there are the usual chapters on components of neural tissues, which once again we have found convenient to divide into enzymes, macromolecules, and other constituents.

• attention
• brain
• nervous system
• neurochemistry

• New York State Research Institute for Neurochemistry and Drug Addiction, New York, USA

  Neville Marks

• Department of Biochemistry, Institute of Psychiatry University of London, London, Great Britain

  Richard Rodnight

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