In Vitro Fertilization and Embryo Transfer

1. Front Matter

   Pages i-x

   PDF

2. Human IVF-ET: State of the Art 1987

   • Don P. Wolf

   Pages 1-6

3. Selection and Use of Equipment

   • Marybeth Gerrity

   Pages 7-24

4. Quality Control and Laboratory Monitoring

   • Marybeth Gerrity

   Pages 25-45

5. Choice, Preparation and use of Culture Medium

   • Gregory S. Kopf

   Pages 47-56

6. Mouse Embryo Culture Bioassay

   • Marybeth Gerrity

   Pages 57-76

7. Preparation and Analysis of Semen Samples

   • Gregory S. Kopf

   Pages 77-102

8. Assessment of Human Sperm Fertility Potential

   • Don P. Wolf

   Pages 103-124

9. Analysis of Oocyte Quality and Fertilization

   • Don P. Wolf

   Pages 125-136

10. Analysis of Embryonic Development

    • Don P. Wolf

    Pages 137-145

11. Morphology of Human Eggs and Embryos

    • Don P. Wolf, Marybeth Gerrity, Gregory S. Kopf

    Pages 147-188

12. Embryo Transfer

    • Marybeth Gerrity, John S. Rinehart

    Pages 189-206

13. Animal Models for the Study of Fertilization and Early Development in Vitro

    • Barry D. Bavister

    Pages 207-234

14. Data Collection and Management

    • Gregory S. Kopf

    Pages 235-264

15. Gamete Intrafallopian Transfer (GIFT)

    • William Byrd

    Pages 265-284

16. Endocrine Measurements

    • Albert S. Berkowitz

    Pages 285-300

17. Cryopreservation of Mammalian Embryos

    • Frank B. Kuzan, Patrick Quinn

    Pages 301-347

18. Control Mechanisms Regulating Meiotic Maturation of Mammalian Oocytes

    • Richard Schultz

    Pages 349-358

19. Regulation of Embryonic Development by Environmental Factors

    • Michael T. Kane

    Pages 359-387

20. Clinical Parameters Influencing Success in IVF

    • Sander S. Shapiro

    Pages 389-408

The use of human in vitro fertilization in the management of infertility is the outgrowth of years of laboratory observations on in vitro sperm-egg interaction. "The editors of this work have themselves contributed significantly to basic knowledge of the mammalian fertilization process. The observations of Don Wolf on sperm penetration, the block to polyspermy and, most recently, sperm hyperactivation in the monkey and human, Gregory Kopf's elucidation of the mechanisms of sperm activation during penetration and the reciprocal dialogue between sperm and egg, and Barry Bavister's definition of culture conditions and requirements necessary for in vitro oocyte maturation, fertilization and development in model mammalian systems including nonhuman primates have contributed greatly to our understanding of the mammalian fertilization process. Wolf, Kopf and Gerrity have enjoyed substantial interaction with clinicians in Departments of Obstetrics and Gynecology and have been directly involved with successful IVF programs. Both Wolf and Kopf have served as research scientists in the Division of Reproductive Biology at the University of Pennsylvania, which, for more than 22 years, has fostered co-mingling of clinically oriented and basic science faculty. It is through such interaction, which clearly exists at many institutions including the University of Wisconsin, that the process of technology transfer is best served. Without an exquisitely coordinated laboratory, there can be no consistent success in human in vitro fertilization. Quality control is pivotal, but close collaboration between the laboratory and the clinic is also essential as information is shared and correlated.

• biology
• development
• embryo
• fertility
• in vitro fertilization
• infertility
• monitoring
• primates
• regulation
• reproductive biology

• Oregon Regional Primate Research Center, Beaverton, USA

  Don P. Wolf

• Wisconsin Regional Primate Research Center, Madison, USA

  Barry D. Bavister

• Evanston and Glenbrook Hospitals, Evanston, USA

  Marybeth Gerrity

• University of Pennsylvania, Philadelphia, USA

  Gregory S. Kopf

Policies and ethics