NMR as a Structural Tool for Macromolecules

1. Front Matter

   Pages i-x

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2. The Theme of the Symposium

   • B. D. Nageswara Rao

   Pages 1-13

3. Intramolecular Dynamics of Biomolecules, Possibilities and Limitations of NMR

   • R. R. Ernst, M. J. Blackledge, T. Bremi, R. Brüschweiler, M. Ernst, C. Griesinger et al.

   Pages 15-30

4. Structural, Dynamic, and Folding Studies of SH2 and SH3 Domains

   • Julie D. Forman-Kay, Steven M. Pascal, Alex U. Singer, Toshio Yamazaki, Ouwen Zhang, Neil A. Farrow et al.

   Pages 35-47

5. NMR Studies of Proteins Involved in Cell Adhesion Processes

   • Gerhard Wagner, Daniel F. Wyss, Johnathan S. Choi, Antonio R. N. Arulanandam, Ellis L. Reinherz, Andrzej Krezel et al.

   Pages 51-62

6. Combining 2H and 13C Selective Enrichment to Probe Protein Dynamics

   • David M. LeMaster

   Pages 65-76

7. Incorporating Motional Properties into the Interpretation of Three-dimensional Solution Structures

   • Walter J. Chazin

   Pages 77-86

8. Phosphotyrosyl Peptide-Enzyme Complexes: How Much Structure Can We Get From Transferred Noe’s

   • Carol Beth Post, Michael L. Schneider

   Pages 91-99

9. Panel Discussion

   • David Case, Marvin D. Kemple, N. Rama Krishna, Carol B. Post, Gerhard Wagner

   Pages 103-116

10. Recent Developments in Protein NMR Spectroscopy

    • Stephan Grzesiek, Geerten W. Vuister, Andy C. Wang, Frank Delaglio, Ad Bax

    Pages 117-120

11. Field-Cycling NMR Applied to Macromolecular Structure and Dynamics

    • Alfred G. Redfield

    Pages 123-132

12. Cross-Correlations: Obstacles or Tools for Structure Determination of Biomolecules

    • Anil Kumar

    Pages 135-142

13. Towards the Accurate Measurement of Internuclear Distances in Biological Macromolecules by Suppression of Spin Diffusion

    • Sébastien J. F. Vincent, Catherine Zwahlen, Geoffrey Bodenhausen

    Pages 145-164

14. NMR of Symmetrical Assemblies of Self-Recognizing Oligonucleotides

    • Maurice Guéron, Kalle Gehring, Jean-Louis Leroy

    Pages 167-171

15. Protein-DNA Interaction from NMR and Monte Carlo Docking

    • R. Kaptein, M. Slijper, V. P. Chuprina, J. A. C. Rullmann, R. M. A. Knegtel, R. Boelens

    Pages 175-187

16. Dynamic Structure of Nucleic Acid Duplexes

    • Thomas L. James, Carlos González, He Liu, Uli Schmitz, Nikolai B. Ulyanov

    Pages 191-203

17. Panel Discussion

    • G. Marius Clore, Stephen W. Fesik, David G. Gorenstein, David M. LeMaster, John L. Markley

    Pages 207-219

18. NMR Structures of Proteins Involved in Signal Transduction

    • S. W. Fesik, R. P. Meadows, E. T. Olejniczak, A. P. Petros, P. J. Hajduk, H. S. Yoon et al.

    Pages 221-234

19. Structures of Multimeric Proteins by NMR

    • G. Marius Clore, Angela M. Gronenborn

    Pages 237-242

20. NMR Structural Studies of Flexible Molecules

    • Peter E. Wright, H. Jane Dyson

    Pages 245-249

The contemplation of truth and beauty is the proper object for which we were created, which calls forth the most intense desires of the soul, and of which it never tires -Hazlitt In his Nobel lecture Purcell commented that when he saw snow in New England after the discovery of NMR, it appeared like "heaps of protons quietly precessing in earth's magnetic field. " If he were to make the comment in the context of how NMR is being used today, he could have conjured up an image of hydrogen, carbon, and nitrogen nuclei in proteins of an earthbound 8rganism subtly orchestrating a quiet symphony of frequencies, from 150 Hz to 2 kHz, carrying clues to the three-dimensional structure of the macromolecules. The manner in which the basic discoveries of Bloch and Purcell have led to the emergence of NMR, several decades later, as a major technique of biological and medical physics (and chemistry) is a striking example of the power of basic research. It is also a fascinating saga whereby whenever it was felt that the field had reached a plateau, new directions, new technologies, and sometimes serendipity produced new developments that revolutionized the technique and enhanced its capability. As Richard Ernst points out "NMR is intellectually attractive, . . . the practical importance of NMR is enormous, and can justify much of the playful activities of an addicted spectroscopist" (Nobel lecture).

• Calcium
• Nucleotide
• Peptide
• chemistry
• medical physics
• protein
• proteins
• spectroscopy

• Indiana University-Purdue University, Indianapolis (IUPUI), Indianapolis, USA

  B. D. Nageswara Rao, Marvin D. Kemple

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