Skip to main content

Recombinant DNA Research and Viruses

Cloning and Expression of Viral Genes

  • Book
  • © 1985

Overview

Part of the book series: Developments in Molecular Virology (DMVI, volume 5)

This is a preview of subscription content, log in via an institution to check access.

Access this book

eBook USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (18 chapters)

  1. Cloning and Transfer of Viral Genes

  2. Genetic Engineering Strategy

  3. Cloning of DNA Virus Genes

  4. Cloning of Retrovirus DNA

  5. Cloning of the DNA form of an RNA Virus Genome

  6. Cloning of Plant Viruses and Viroid Genes

Keywords

About this book

The development of recombinant DNA technology has made a marked impact on molecular virology. The cleavage of viral DNA genomes with restriction enzymes and the cloning of such DNA fragments in bacterial p1asmids has led to the amplification of selected viral DNA fragments for sequencing and gene expression. RNA virus genomes which can be transcribed to their cDNA form were also cloned in bacterial p1asmids, facilitating the study of RNA virus genes. With the elucidation in recent years of the promoter sequence of various viral genes and the expression of these genes in bacteria or yeast, the understanding of many viral gene functions has made great progress. Cloning and expression of viral genes in mammalian cells was made possible by the construction of shuttle plasmid vectors which carry the origins of DNA replication from bacteria and/or mammalian viruses. The expression of viral genes in bacteria, yeast and eukaryotic cells gives reason to hope that it will be possible to produce viral antigens in large quantities for use as human or animal vaccines. The present volume attempts to capture for the reader some of the high­ lights of recombinant DNA research in the field of animal and plant viruses.

Editors and Affiliations

  • The Hebrew University of Jerusalem, Israel

    Yechiel Becker

Bibliographic Information

  • Book Title: Recombinant DNA Research and Viruses

  • Book Subtitle: Cloning and Expression of Viral Genes

  • Editors: Yechiel Becker, Julia Hadar

  • Series Title: Developments in Molecular Virology

  • DOI: https://doi.org/10.1007/978-1-4613-2565-9

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Book Archive

  • Copyright Information: Martinus Nijhoff Publishing, Boston 1985

  • Hardcover ISBN: 978-0-89838-683-7Published: 31 December 1984

  • Softcover ISBN: 978-1-4612-9611-9Published: 27 September 2011

  • eBook ISBN: 978-1-4613-2565-9Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: X, 374

  • Topics: Virology

Publish with us