Chemical Mutagens

1. Front Matter

   Pages i-xiv

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2. The Grasshopper Neuroblast Short-Term Assay for Evaluating the Effects of Environmental Chemicals on Chromosomes and Cell Kinetics

   • Mary Esther Gaulden, Jan C. Liang, Martha J. Ferguson

   Pages 1-65

3. Comparison of the Mutagenic Responses of Lung-Derived and Skin-Derived Human Diploid Fibroblast Populations

   • Lois Jacobs, James A. Marx, Christian L. Bean

   Pages 67-88

4. The L-Arabinose Resistance Test with Salmonella typhimurium

   • Carmen Pueyo, Manuel Ruiz-Rubio

   Pages 89-109

5. Identification of Mutagens from the Cooking of Food

   • Frederick T. Hatch, James S. Felton, Daniel H. Stuermer, Leonard F. Bjeldanes

   Pages 111-164

6. The Bacillus subtilis Multigene Sporulation Test for Detection of Environmental Mutagens

   • L. E. Sacks, J. T. MacGregor

   Pages 165-181

7. The L5178Y/TK Gene Mutation Assay System

   • David E. Amacher

   Pages 183-212

8. Induction of Bacteriophage Lambda by DNA-Interacting Chemicals

   • Rosalie K. Elespuru

   Pages 213-231

9. The Granuloma Pouch Assay

   • Peter Maier

   Pages 233-260

10. The Use of Multiply Marked Escherichia coli K12 Strains in the Host-Mediated Assay

    • G. R. Mohn, P. R. M. Kerklaan, P. A. van Elburg

    Pages 261-282

11. The Detection of Mutagens in Human Feces as an Approach to the Discovery of Causes of Colon Cancer

    • H. F. Mower

    Pages 283-294

12. Chromosomal Causes for Fertility Reduction in Mammals

    • Peter de Boer

    Pages 427-467

13. Mutagenesis and Plasmids

    • Kristien E. Mortelmans, Linda Dousman

    Pages 469-507

14. Mutation in Somatic Cells as Determined by Electrophoretic Analysis of Mutagen-Exposed Chinese Hamster Ovary Cells

    • Michael J. Siciliano, Raymond L. Stallings, Ronald M. Humphrey, Gerald M. Adair

    Pages 509-531

15. Back Matter

    Pages 295-306

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Volume 9 of Chemical Mutagens consists mainly of chapters discussing the development and validation of short-term assays to detect the mutagenic effects of environmental chemicals. These chapters include an assay with the grasshopper neuroblast, a comparison of mutagenic responses of human lung-derived and skin-derived diploid fibroblasts, a forward-mutation assay in Salmonella, a multigene sporulation test in Bacillus subtilis, a specific locus assay in mouse lymphoma cells, a study of the induction of bacteriophage lambda, and the granuloma pouch assay. In addition, there are two chapters on the identification of mutagens in cooked food and in human feces. Frederick 1. de Serres Research Triangle Park, North Carolina vii Contents Chapter 1 The Grasshopper Neuroblast Short-Term Assay for Evaluating the Effects of Environmental Chemicals on Chromosomes and Cell Kinetics 1 Mary Esther Gaulden, Jan C. Liang, and Martha J. Ferguson 1. Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 2. Embryo Supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 2. 1. Species. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 2. 2. Origin of Colonies . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 2. 3. Life Cycle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 2. 4. Colony Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . 6 2. 5. Pathology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 2. 6. Allergy to Grasshoppers . . . . . . . . . . . . . . . . . . . . . . 14 3. Grasshopper Egg, Embryo, and Cells . . . . . . . . . . . . . . . . . 14 3. 1. The Egg Shell and Membranes . . . . . . . . . . . . . . . . . 14 3. 2. Embryonic Development . . . . . . . .. . . . . . . . . . . . . . 17 3. 3. Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 4. Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 4. 1. Exposure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 4. 2. Preparation of Embryos for Cell Analysis . . . . . . . . . 34 4. 3. Analysis of Mutagen Effects. . . . . . . . . . . . . . . . . 40 . . . 5. Response of the Grasshopper Neuroblast to Mutagens . . . . 50 5. 1. Reproducibility of Data . . . . . . . . . . . . . . . . . . . . . . . 50 5. 2. Radiation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 5. 3. Chemical Mutagens . . . . . . . . . . . . . . . . . . . . . . . . . .

• Chromosom
• DNA
• biology
• cell lines
• genotypes
• mercury
• toxicity
• ecotoxicology

• National Institute of Environmental Health Sciences, USA

  Frederick J. Serres

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