Gene Quantification

1. Front Matter

   Pages i-xiv

   PDF

2. Key Issues, Challenges, and Future Opportunities in Gene Quantification

   1. Key Issues, Challenges, and Future Opportunities in Gene Quantification

      • François Ferré

      Pages 1-16

3. Methods/Technology Issues

   1. Front Matter

      Pages 17-17

      PDF

   2. Gene Quantitation Based on PCR Amplification

      1. Present and Future Detection Formats for PCR Quantitation of Nucleic Acids

         • Shirley Kwok, John Sninsky

         Pages 19-30

      2. Determination of Target Copy Number of Quantitative Standards Used in PCR-Based Diagnostic Assays

         • Zhuang Wang, Joanne Spadoro

         Pages 31-43

      3. Quantification of Specific Nucleic Acids, Regulated RNA Processing, and Genomic Polymorphisms Using Reversed-Phase HPLC

         • A. Hayward-Lester, B. S. Chilton, P. A. Underhill, P. J. Oefner, P. A. Doris

         Pages 45-78

      4. Capillary Electrophoresis for Quantitative Genetic Analysis

         • Jill M. Kolesar, John G. Kuhn

         Pages 79-96

      5. Quantitative PCR Technology

         • Lincoln McBride, Ken Livak, Mike Lucero, Federico Goodsaid, Dane Carlson, Junko Stevens et al.

         Pages 97-110

      6. Statistical Estimations of PCR Amplification Rates

         • Jean Peccoud, Christine Jacob

         Pages 111-128

      7. Fluorescence Monitoring of Rapid Cycle PCR for Quantification

         • Carl Wittwer, Kirk Ririe, Randy Rasmussen

         Pages 129-144

      8. Kinetic ELISA-PCR: A Versatile Quantitative PCR Method

         • Olivier Lantz, Elizabeth Bonney, Scott Umlauf, Yassine Taoufik

         Pages 145-165

   3. Gene Quantitation Based on Other Target Amplification Systems

      1. Quantitation of RNA by NASBA™: Applications and Issues for HIV-1 and AIDS

         • Joseph Romano, Paul van de Wiel, Stuart Geiger

         Pages 169-188

      2. Application of Transcription-Mediated Amplification to Quantification of Gene Sequences

         • Frank Gonzales, Sherrol H. McDonough

         Pages 189-201

   4. Gene Quantitation Based on Signal Amplification

      1. Branched DNA (bDNA) Technology for Direct Quantification of Nucleic Acids: Design and Performance

         • Mark L. Collins, Peter J. Dailey, Lu-Ping Shen, Mickey S. Urdea, Linda J. Wuestehube, Janice A. Kolberg

         Pages 205-223

      2. Hybrid Capture™ — A Sensitive Signal-Amplified Test for the Detection and Quantitation of Human Viral and Bacterial Pathogens

         • Attila T. Lörincz, Mariana G. Meijide, James G. Lazar, Abel De La Rosa

         Pages 225-249

4. Applications

   1. Front Matter

      Pages 251-251

      PDF

   2. Quantification of Gene Expression by Competitive RT-PCR: The hCGβ/LH/β Gene Cluster

      • Vladimir Lazar, Ivan Bièche, Michel Bahuau, Yves Giovangrandi, Dominique Bellet, Michel Vidaud

      Pages 253-264

   3. Quantitative Detection of Mycoplasma DNA Using Competitive PCR

      • Maninder K. Sidhu, Mei-June Liao, Abbas Rashidbaigi

      Pages 265-276

   4. The Detection and Quantification of bcr-abl in Chronic Myeloid Leukemia Following Marrow Transplantation

      • Jerald Radich

      Pages 277-293

   5. Competitive RT-PCR Analysis of Brain Gene Expression During Inflammation and Disease

      • Douglas L. Feinstein, Elena Galea

      Pages 295-312

Geneticists and molecular biologists have been interested in quantifying genes and their products for many years and for various reasons (Bishop, 1974). Early molecular methods were based on molecular hybridization, and were devised shortly after Marmur and Doty (1961) first showed that denaturation of the double helix could be reversed - that the process of molecular reassociation was exquisitely sequence dependent. Gillespie and Spiegelman (1965) developed a way of using the method to titrate the number of copies of a probe within a target sequence in which the target sequence was fixed to a membrane support prior to hybridization with the probe - typically a RNA. Thus, this was a precursor to many of the methods still in use, and indeed under development, today. Early examples of the application of these methods included the measurement of the copy numbers in gene families such as the ribosomal genes and the immunoglo­ bulin family. Amplification of genes in tumors and in response to drug treatment was discovered by this method. In the same period, methods were invented for estimating gene num­ bers based on the kinetics of the reassociation process - the so-called Cot analysis. This method, which exploits the dependence of the rate of reassociation on the concentration of the two strands, revealed the presence of repeated sequences in the DNA of higher eukaryotes (Britten and Kohne, 1968). An adaptation to RNA, Rot analysis (Melli and Bishop, 1969), was used to measure the abundance of RNAs in a mixed population.

• DNA
• Expression
• PCR
• Pathogen
• Polymerasekettenreaktion
• Quantitative PCR
• RNA
• Termination
• biology
• development
• gene expression
• genes
• hybridization
• membrane
• transcription
• biochemical engineering

• The Immune Response Corporation, Carlsbad, USA

  François Ferré

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