Rapid Methods in Clinical Microbiology

1. Front Matter

   Pages i-xii

   PDF

2. “Where we’ve been and where we may be going”

   • Henry D. Isenberg

   Pages 1-9

3. The Use of DNA Probes to Detect and Identify Microorganisms

   • David E. Kohne

   Pages 11-35

4. The Influence of Rapid Diagnosis of Streptococcal Infection on Pharyngitis and Rheumatic Fever

   • Joseph M. Campos

   Pages 37-50

5. The Use of DNA Probes for Rapidly Identifying Cultures of Mycobacterium

   • Charles L. Woodley, Vella A. Silcox, Margaret M. Floyd, George P. Kubica

   Pages 51-56

6. Use of DNA Probes for the Diagnosis of Infections Caused by Mycoplasma Pneumoniae and Legionellae- A Review

   • Paul H. Edelstein

   Pages 57-69

7. Multicenter Clinical Evaluation of the Du Pont HERPCHEKTM HSV Elisa, a New Rapid Diagnostic Test for the Direct Detection of Herpes Simplex Virus

   • David A. Baker, Deborah Pavan-Langston, Bernard Gonik, Peter O. Milch, Edmund C. Dunkel, Albert Berkowitz et al.

   Pages 71-76

8. Non-Culture Tests for the Diagnosis of Gonorrhea

   • Leonard Zubrzycki

   Pages 77-88

9. Nucleic Acid Hybridization as a Diagnostic Tool for the Detection of Human Papillomaviruses

   • Robert F. Rando

   Pages 89-109

10. The Rapid Detection of Clinically Significant Bacteria

    • Richard C. Tilton

    Pages 111-114

11. Rapid Diagnosis of Viral Infections

    • Thomas F. Smith

    Pages 115-121

12. Time-Resolved Fluorometry: Principles and Application to Clinical Microbiology and DNA Probe Technology

    • Pertti Hurskainen, Patrik Dahlén, Harri Siitari, Timo Lövgren

    Pages 123-130

13. The Clinical Impact of Automated Susceptibility Reporting Using a Computer Interface

    • Alan T. Evangelista

    Pages 131-142

14. Back Matter

    Pages 143-146

    PDF

The papers published herein comprise the presentations given at the eighteenth of an annual series of clinical symposia arranged under the auspices of the Eastern Pennsylvania Branch of the American Society for Microbiology. This symposium allowed approximately 200 persons to gather and exchange ideas on the rapid laboratory diagnosis of infectious diseases. The institution of the Diagnosis Related Group (DRG) method for reimbursement by both government agencies and private insurance carriers has provided a financial aspect to the established clinical reasons for rapid laboratory diagnosis. Now the health of the institution, as well as the patient, is dependent on a timely diagnosis and, hopefully, cure. Accordingly, the goal of this symposium was to present the latest developments in "same-day microbiology". In the face of stable or diminishing resources, the laboratory director is presented with many choices. Do nucleic acid probes, non­ instrumental ELISA techniques, or time-resolved fluorometry have a place in his or her laboratory? Should the laboratory test for newly described human pathogens such as human immunodeficiency virus or human papilloma virus? Can rapid techniques supplant conventional methods? Or are they merely adjunctive? This symposium attempted to assist in the formulation of informed decisions. Bruce Kleger Donald Jungkind Eileen Rinks Linda A. Miller vii ACKNOWLEDGEMENTS We would like to thank the Eastern Pennsylvania Branch of t~e American Society for Microbiology for sponsoring this symposium and for making this publication possible. We especially thank the Symposium Committee for their diligent work in organizing an informative and successful symposium.

• Laboratory
• immunodeficiency
• infection
• infectious disease
• microbiology
• microorganism
• virus

• Pennsylvania Department of Health, Lionville, USA

  Bruce Kleger

• Thomas Jefferson University, Philadelphia, USA

  Donald Jungkind

• United Hospitals, Inc., Cheltenham, USA

  Eileen Hinks

• Holy Redeemer Hospital and Medical Center, Meadowbrook, USA

  Linda A. Miller

Policies and ethics