Receptor Purification

1. Front Matter

   Pages i-xiii

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2. Serotonin Receptors

   • Timothy K. Gallaher, Howard H. Wang

   Pages 1-21

3. Purification and Cloning of Central Serotonin Receptors

   • Henri Gozlan, Salah El Mestikawy, Michel Hamon

   Pages 23-43

4. Purification of β-Adrenergic Receptors

   • Craig C. Malbon

   Pages 45-75

5. Purification of β,γ Subunits of Guanine Nucleotide Binding Proteins

   • Deirdre Cooney, Alan K. Keenan

   Pages 77-98

6. Purification of Opioid Binding Proteins

   • Theresa L. Gioannini, Eric J. Simon

   Pages 99-113

7. µ-Opioid Receptor

   • Hiroshi Ueda, Masamichi Satoh

   Pages 115-129

8. The Neurotensin Receptor from Mammalian Brain

   • Jean-Pierre Vincent, Jean Mazella, Joëlle Chabry, Nicole Zsurger

   Pages 131-145

9. The Luteinizing Hormone/Human Chorionic Gonadotropin Receptor of Testis and Ovary

   • Maria L. Dufau

   Pages 147-171

10. Purification of LH/hCG Receptor

    • Om P. Bahl, Hakimuddin T. Sojar

    Pages 173-190

11. The Follicle Stimulating Hormone Receptor

    • Bosukonda Dattatreyamurty, Leo E. Reichert Jr.

    Pages 191-215

12. Thyrotropin Receptor

    • Takashi Akamizu, Michele De Luca, Leonard D. Kohn

    Pages 217-248

13. Thyrotropin Receptor Purification

    • Petu J. Leedman, Leonard C. Harrison

    Pages 249-268

14. Prolactin Receptors

    • Arieh Gertler

    Pages 269-288

15. Ligand- and Antibody-Affinity Purification of the Epidermal Growth Factor Receptor

    • George Panayotou, J. Justin Hsuan, Michael D. Waterfield

    Pages 289-302

16. Platelet-Derived Growth Factor B Type Receptor

    • Carl-Henrik Heldin, Lars Rönnstrand

    Pages 303-314

17. Colony-Stimulating Factor 1 Receptor

    • Yee-Guide Yeung, E. Richard Stanley

    Pages 315-328

18. Insulin-Like Growth Factor-II Receptors

    • Robert C. Baxter, Carolyn D. Scott

    Pages 329-346

19. Purification of Insulin and Insulin-Like Growth Factor (IGF)-I Receptor

    • Yoko Fujita-Yamaguchi, Thomas R. Le Bon

    Pages 347-371

20. Insulin Receptor Isolation Through Avidin-Biotin Technology

    • Frances M. Finn, Klaus Hofmann

    Pages 373-402

The purpose of these volumes is to provide a reference work for the methods of purifying many of the receptors we know about. This be­ comes increasingly important as full-length receptors are overexpressed in bacteria or in insect cell systems. A major problem for abundantly expressed proteins will be their purification. In addition to purification protocols, many other details can be found concerning an individual receptor that may not be available in standard texts or monographs. No book of this type is available as a compendium of purification procedures. Receptor Purification provides protocols for the purification of a wide variety of receptors. These include receptors that bind: neurotransmit­ ters, polypeptide hormones, steroid hormones, and ligands for related members of the steroid supergene family and others, including receptors involved in bacterial motion. The text of this information is substantial, so as to require its publication in two volumes. Consequently, a division was made by grouping receptors by the nature of their ligands. Thus, in Volume One there are contributions on serotonin receptors, adrenergic receptors, the purification of GTP-binding proteins, opioid receptors, neurotensin receptor, luteinizing hormone receptor, human chorionic gonadotropin receptor, follicle stimulating hormone receptor, thyro­ tropin receptor, prolactin receptor, epidermal growth factor receptor, platelet derived growth factor receptor, colony stimulating factor recep­ tor, insulin-like growth factor receptors, insulin receptor, fibronectin receptor, interferon receptor, and the cholecystokinin receptor.

• G proteins
• Vitamin D
• attention
• bacteria
• growth
• growth factor
• hormone
• insulin-like
• interferon
• neurotransmitter
• protein
• proteins
• receptor
• steroid
• tissue

• Fels Institute for Cancer Research and Molecular Biology, Philadelphia, USA

  Gerald Litwack

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