Mass Spectrometry in the Biological Sciences

1. Front Matter

   Pages i-xii

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2. Mass Spectrometry in the Health and Life Sciences

   • Judith L. Vaitukaitis, Maureen Mylander

   Pages 1-4

3. Designing Time-of-Flight Mass Spectrometers for the Future: Multichannel Recording of Peptide Amino Acid Sequences

   • Robert J. Cotter, Timothy J. Cornish, Marcela Cordero

   Pages 5-23

4. The Role of Fourier Transform Ion Cyclotron Resonance Mass Spectrometry in Biological Research — New Developments and Applications

   • R. D. Smith, J. E. Bruce, Q. Wu, X. Cheng, S. A. Hofstadler, G. A. Anderson et al.

   Pages 25-68

5. Electrospray Ionization Mass Spectrometry: The Observation of Covalent, Ionic and Non-Covalent Interactions.

   • Robin T. Aplin, Carol V. Robinson

   Pages 69-84

6. Using Deuterium Exchange and Mass Spectrometry to Study Protein Structure

   • Robert J. Anderegg

   Pages 85-104

7. MALDI-TOF Mass Spectrometry in the Protein Biochemistry Lab: From Characterization of Cell Cycle Regulators to the Quest for Novel Antibiotics

   • Paul Tempst, Hediye Erdjument-Bromage, Peter Casteels, Scott Geromanos, Mary Lui, Michael Powell et al.

   Pages 105-133

8. Chemistry, Mass Spectrometry and Peptide-Mass Databases: Evolution of Methods for the Rapid Identification and Mapping of Cellular Proteins

   • D. J. C. Pappin, D. Rahman, H. F. Hansen, M. Bartlet-Jones, W. Jeffery, A. J. Bleasby

   Pages 135-150

9. Identification of Electroblotted Proteins by Peptide Mass Searching of a Sequence Database

   • John T. Stults, William J. Henzel, Susan C. Wong, Colin Watanabe

   Pages 151-170

10. Mass Spectrometric Identification of Proteins Isolated By Two-Dimensional Gel Electrophoresis

    • Steven C. Hall, Karl R. Clauser, Lori E. Andrews, Fred C. Walls, Diana M. Smith, Huu M. Tran et al.

    Pages 171-202

11. Infrared-Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (IR-MALDI-MS) of Proteins Electroblotted onto Polymer Membranes After SDS-PAGE Separation

    • Kerstin Strupat, Michael Karas, Franz Hillenkamp, Christoph Eckerskorn

    Pages 203-216

12. Picoliter Vials: A New Sample Handling Technique for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

    • S. Jespersen, W. M. A. Niessen, U. R. Tjaden, J. van der Greef, E. Litborn, U. Lindberg et al.

    Pages 217-226

13. Direct Neuropeptide Profiling of Single Neurons and Target Tissue by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

    • C. R. Jiménez, K. W. Li, A. B. Smit, J. van Minnen, C. Janse, W. P. M. Geraerts et al.

    Pages 227-243

14. Approaches to the Practical Use of MS/MS in a Protein Sequencing Facility

    • M. Wilm, T. Houthaeve, G. Talbo, R. Kellner, P. Mortensen, M. Mann

    Pages 245-265

15. Capillary Liquid Chromatography/Electrospray Mass Spectrometry with a Magnetic Sector Instrument for Accurate Mass Measurement of Peptide and Protein Mixtures

    • Hiroshi Murata, Toshifumi Takao, Yasutsugu Shimonishi

    Pages 267-280

16. Mass Spectrometric Methods for Peptide Sequencing: Applications to Immunology and Protein Acylation

    • Pamela H. Gulden, Murray Hackett, Theresa A. Addona, Lin Guo, Carthene B. Walker, Nicholas E. Sherman et al.

    Pages 281-305

17. A Multifaceted Strategy for the Characterization of Recombinant gD-2, a Potential Herpes Vaccine

    • Mark E. Hemling, Mary A. Mentzer, Steven A. Carr, Carine Capiau

    Pages 307-331

18. Identifying Unexpected Protein Modifications

    • Reed J. Harris, Michael S. Molony, May Y. Kwong, Victor T. Ling

    Pages 333-350

19. Mass Spectrometry in Carbohydrate Recognition Studies

    • A. M. Lawson, W. Chai, C.-T. Yuen, T. Feizi

    Pages 351-364

20. Mass Spectrometric and Tandem Mass Spectrometric Approaches to the Analysis of Glycoconjugates

    • Catherine E. Costello, Hélène Perreault, Lambert C. Ngoka

    Pages 365-384

Leading practitioners describe in detail advanced methods of mass spectrometry used in structural characterization of biomacromolecules of both natural and recombinant origin. They demonstrate by example how these methodologies can solve a wide array of real-world problems in protein biochemistry, immunology, and glycobiology, as well as for human bacterial pathogens, lipids, and nucleic acids. The book offers a unique opportunity to learn these techniques that are revolutionizing the field. Its authoritative assessment in the context of how to solve important and challenging problems in bioscience and medicine ensures a competitive advantage for today's researchers.

• Amino acid
• DNA
• Mass Spectrometry
• Oligosaccharid
• Oxidation
• biochemistry
• transcription

The articles are uniformly well written...In summary, the book best serves the community interested in the characterization of proteins by mass spectrometer methods, but it contains valuable insights for analysis of other biomolecules as well.- Quarterly Review of Biology

• University of California, San Francisco, USA

  A. L. Burlingame

• SmithKline Beecham Pharmaceuticals, King of Prussia, USA

  Steven A. Carr

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