Immunocytochemistry and In Situ Hybridization in the Biomedical Sciences

1. Front Matter

   Pages i-xi

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2. Introduction

   • Julian E. Beesley

   Pages 1-5

3. Preservation of Tissue for Immunocytochemical Studies

   • Iolo Ap Gwynn

   Pages 6-29

4. Specimen Preparation for Immunocytochemistry

   • Gillian Brown, Anthony Wong, Julian E. Beesley

   Pages 30-54

5. Antigen Retrieval

   • Peter Jackson, David Blythe

   Pages 55-72

6. Immunolabeling

   • Alexander D. Hyatt, Terry G. Wise

   Pages 73-107

7. In Situ Hybridization: General Principles and Application of Digoxigenin-Labeled cRNA for the Detection of mRNAs

   • Eveline Baumgart, Arno Schad, Markus Grabenbauer

   Pages 108-137

8. Fluorescence In Situ Hybridization

   • Chee Gee See

   Pages 138-155

9. In Situ Amplification and Detection of Nucleic Acids

   • Alfredo Martínez, Yan-gao Man, Steven J. Zullo, Frank Cuttitta

   Pages 156-174

10. Image Analysis and Statistics: A Quantitative Approach to Immunocytochemistry and Related Techniques

    • Suzanna J. Newman, Paul Nelson

    Pages 175-199

11. Safety in the Laboratory

    • Michael Hurle

    Pages 200-222

12. Applications of Affinity Labeling in Biomedical Sciences

    • David A. Hughes

    Pages 223-253

13. Back Matter

    Pages 255-267

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Immunocytochemistry and in situ hybridization are widely used biomedical sciences. They are essential in medical diagnosis and in cell biology research. Affinity labeling is the central goal of the experimental strategy involving a series of techniques in a logical order; from the effects of specimen fixation, through specimen preparation to expose the antigen, to optimizing immunolabeling, to assessing the result and finally to safety considerations. Numerous examples of these techniques in biomedical sciences are included, as well as experimental assays and practical tips. This survey of methods will serve as an invaluable reference source in any laboratory setting (academic, industrial or clinical) involved in research in almost every branch of biology or medicine, as well as in pharmaceutical, biotechnological and clinical applications.

• Antigen
• FISH
• PCR
• biology
• cell
• cell biology
• chemistry
• hybridization
• image analysis
• molecular biology
• nucleic acid
• tissue

• Cell Biology, Glaxo Wellcome Medicines Research Centre, Stevenage, Hertfordshire, UK

  Julian E. Beesley

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