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Methods in Protein Sequence Analysis

  • Book
  • © 1982

Overview

Editors:
  1. Marshall Elzinga

    1. Brookhaven National Laboratory, Upton, USA

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Part of the book series: Experimental Biology and Medicine (EBAM, volume 3)

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Table of contents (69 chapters)

  1. Front Matter

    Pages i-xxvii
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  2. Conference Lecture

    1. Front Matter

      Pages xxvii-xxvii
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    2. An Anecdotal Account of the History of Peptide Stepwise Degradation Procedures

      • Russell F. Doolittle
      Pages 1-24

  3. Overview

    1. Front Matter

      Pages 25-25
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    2. An Evaluation of the Current Status of Protein Sequencing

      • Brigitte Wittmann-Liebold
      Pages 27-63

  4. Strategy

    1. Front Matter

      Pages 65-65
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    2. Strategic Approaches to Sequence Analysis

      • Kenneth A. Walsh
      Pages 67-73

  5. Improvements in Instrumentation and the Chemistry of Isothiocyanate Degradation

    1. Front Matter

      Pages 75-75
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    2. A New Protein Microsequenator Using Gas Phase Edman Reagents

      • Michael W. Hunkapiller, Rodney M. Hewick, William J. Dreyer, Leroy E. Hood
      Pages 77-90

    3. Towards Longer Degradations on a Sequenator

      • Gerhard Frank
      Pages 91-99

    4. The Nature of Non-Specific Peptide Bond Cleavage During the Isothiocyanate Degradation of Proteins

      • Wolf F. Brandt, Agnes Henschen, Claus Von Holt
      Pages 101-110

  6. Microsequencing

    1. Front Matter

      Pages 111-111
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    2. Isolation and Sequence Determination of Polypeptides at the Picomole Level

      • J. Y. Chang, R. Knecht, D. G. Braun
      Pages 113-118

    3. Methodologies in the Microsequencing

      • Ajit S. Bhown, John E. Mole, J. Claude Bennett
      Pages 119-130

    4. Micro-Sequence Analysis of Ovine Hypothalamic Corticotropin Releasing Factor

      • Joachim Spiess, Jean Rivier, Catherine Rivier, Wylie Vale
      Pages 131-138

    5. Identification of Internal Residues of Lactose Permease of Escherichia Coli by Radiolabel Sequencing of Peptide Mixtures

      • Konrad Beyreuther, Barbara Bieseler, Ruth Ehring, Benno Müller-Hill
      Pages 139-148

    6. The Application of Monoclonal Antibodies to the Microsequencing of Proteins

      • Lawrence K. Duffy, Alexander Kurosky
      Pages 149-156

  7. Solid Phase Sequencing

    1. Front Matter

      Pages 157-157
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    2. Sensitivity Enhancement in Solid-Phase Protein Sequence Analysis: An Examination of Methodology

      • Marcus J. Horn, Alex G. Bonner
      Pages 159-171

    3. Progress in Solid-Phase Sequencing: Extended Degradations of Peptides and Proteins

      • Werner Machleidt, Helmut Hofner
      Pages 173-180
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Keywords

About this book

Methods in Protein Sequence Analysis contains an intensely prac­ tical account of all the new methodology available to scientists carrying out protein and peptide sequencing studies. Many of the striking advances in fields as diverse as immunology, cell motility, and neurochemistry have in fact been fueled by our ever more powerful ability to determine the sequences and structures of key proteins and peptides. It is our hope that the rich array of tech­ niques and methods for sequencing proteins discussed in this volume-methods that generate much of the information crucial to progress in modern biology-will now become accessible to all who can benefit from them. The papers of the present volume constitute the Proceedings of the IVth International Conference on Methods in Protein Se­ quence Analysis, which was held at Brookhaven National Labo­ ratory, Upton, NY, September 21-25, 1981. It was the most recent in a series of biennial conferences, the previous one having been held in Heidelberg, GFR, in 1979. The series was originated by Richard Laursen, and initially dealt with one aspect of the field, solid-phase sequencing. The scope of the meeting was very broad and among the many aspects of protein sequencing discussed were: instrumentation, strategy, chemicals, mass spectrometry, cleavage of proteins and separation of peptides, and solid, liquid, manual, and even "gas­ phase" sequencing.

Editors and Affiliations

  • Brookhaven National Laboratory, Upton, USA

    Marshall Elzinga

Bibliographic Information

  • Book Title: Methods in Protein Sequence Analysis

  • Editors: Marshall Elzinga

  • Series Title: Experimental Biology and Medicine

  • DOI: https://doi.org/10.1007/978-1-4612-5832-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Book Archive

  • Copyright Information: The HUMANA Press Inc. 1982

  • Hardcover ISBN: 978-0-89603-038-1Published: 26 July 1982

  • Softcover ISBN: 978-1-4612-5834-6Published: 17 October 2011

  • eBook ISBN: 978-1-4612-5832-2Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XXVII, 589

  • Topics: Biochemistry, general

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