Overview
- Editors:
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François Ferré
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The Immune Response Corporation, Carlsbad, USA
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Table of contents (20 chapters)
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Key Issues, Challenges, and Future Opportunities in Gene Quantification
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Methods/Technology Issues
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Gene Quantitation Based on PCR Amplification
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- Shirley Kwok, John Sninsky
Pages 19-30
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- Zhuang Wang, Joanne Spadoro
Pages 31-43
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- A. Hayward-Lester, B. S. Chilton, P. A. Underhill, P. J. Oefner, P. A. Doris
Pages 45-78
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- Jill M. Kolesar, John G. Kuhn
Pages 79-96
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- Lincoln McBride, Ken Livak, Mike Lucero, Federico Goodsaid, Dane Carlson, Junko Stevens et al.
Pages 97-110
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- Jean Peccoud, Christine Jacob
Pages 111-128
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- Carl Wittwer, Kirk Ririe, Randy Rasmussen
Pages 129-144
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- Olivier Lantz, Elizabeth Bonney, Scott Umlauf, Yassine Taoufik
Pages 145-165
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Gene Quantitation Based on Other Target Amplification Systems
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- Joseph Romano, Paul van de Wiel, Stuart Geiger
Pages 169-188
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- Frank Gonzales, Sherrol H. McDonough
Pages 189-201
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Gene Quantitation Based on Signal Amplification
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- Mark L. Collins, Peter J. Dailey, Lu-Ping Shen, Mickey S. Urdea, Linda J. Wuestehube, Janice A. Kolberg
Pages 205-223
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- Attila T. Lörincz, Mariana G. Meijide, James G. Lazar, Abel De La Rosa
Pages 225-249
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Applications
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Front Matter
Pages 251-251
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- Vladimir Lazar, Ivan Bièche, Michel Bahuau, Yves Giovangrandi, Dominique Bellet, Michel Vidaud
Pages 253-264
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- Maninder K. Sidhu, Mei-June Liao, Abbas Rashidbaigi
Pages 265-276
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- Douglas L. Feinstein, Elena Galea
Pages 295-312
About this book
Geneticists and molecular biologists have been interested in quantifying genes and their products for many years and for various reasons (Bishop, 1974). Early molecular methods were based on molecular hybridization, and were devised shortly after Marmur and Doty (1961) first showed that denaturation of the double helix could be reversed - that the process of molecular reassociation was exquisitely sequence dependent. Gillespie and Spiegelman (1965) developed a way of using the method to titrate the number of copies of a probe within a target sequence in which the target sequence was fixed to a membrane support prior to hybridization with the probe - typically a RNA. Thus, this was a precursor to many of the methods still in use, and indeed under development, today. Early examples of the application of these methods included the measurement of the copy numbers in gene families such as the ribosomal genes and the immunoglo bulin family. Amplification of genes in tumors and in response to drug treatment was discovered by this method. In the same period, methods were invented for estimating gene num bers based on the kinetics of the reassociation process - the so-called Cot analysis. This method, which exploits the dependence of the rate of reassociation on the concentration of the two strands, revealed the presence of repeated sequences in the DNA of higher eukaryotes (Britten and Kohne, 1968). An adaptation to RNA, Rot analysis (Melli and Bishop, 1969), was used to measure the abundance of RNAs in a mixed population.
Editors and Affiliations
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The Immune Response Corporation, Carlsbad, USA
François Ferré
