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Birkhäuser

Protein Analysis and Purification

Benchtop Techniques

  • Book
  • © 1996

Overview

Authors:
  1. Ian M. Rosenberg

    1. Jackson 715, G.I. Unit, Massachusetts General Hospital, Boston, USA

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  • Each chapter contains a wealth of practical information, including troubleshooting tips, safety icons and references
  • More than 180 protocols included with materials lists, step-by-step instructions and valuable comments on practical issues such as time considerations and safety issues

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Table of contents (11 chapters)

  1. Front Matter

    Pages i-xxvi
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  2. An Overview of This Manual

    • Ian M. Rosenberg
    Pages 1-7

  3. Protein Structure

    • Ian M. Rosenberg
    Pages 8-23

  4. Tracking the Target Protein

    • Ian M. Rosenberg
    Pages 24-62

  5. Electrophoretic Techniques

    • Ian M. Rosenberg
    Pages 63-117

  6. Getting Started with Protein Purification

    • Ian M. Rosenberg
    Pages 118-152

  7. Membrane Proteins

    • Ian M. Rosenberg
    Pages 153-176

  8. Transfer and Detection of Proteins on Membrane Supports

    • Ian M. Rosenberg
    Pages 177-210

  9. Identification of the Target Protein

    • Ian M. Rosenberg
    Pages 211-258

  10. Identifying and Analyzing Posttranslational Modifications

    • Ian M. Rosenberg
    Pages 259-323

  11. Chromatography

    • Ian M. Rosenberg
    Pages 324-384

  12. Recombinant Protein Techniques

    • Ian M. Rosenberg
    Pages 385-430

  13. Back Matter

    Pages 431-520
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Keywords

About this book

How one goes about analyzing proteins is a constantly evolving field that is no longer solely the domain of the protein biochemist. Investi­ gators from diverse disciplines find themselves with the unanticipated task of identifying and analyzing a protein and studying its physical properties and biochemical interactions. In most cases, the ultimate goal remains understanding the role(s) that the target protein is playing in cellular physiology. It was my intention that this manual would make the initial steps in the discovery process less time consuming and less intimidating. This book is not meant to be read from cover to cover. The expanded Table of Contents and the index should help locate what you are seeking. My aim was to provide practically oriented information that will assist the experimentalist in benchtop problem solving. The appendices are filled with diverse information gleaned from catalogs, handbooks, and manuals that are presented in a distilled fashion designed to save trips to the library and calls to technical service representatives. The user is encouraged to expand on the tables and charts to fit individual experimental situations. This second edition pays homage to the computer explosion and the various genome projects that have revolutionized how benchtop scientific research is performed. Bioinformatics and In silica science are here to stay. However, the second edition still includes recipes for preparing buffers and methods for lysing cells.

Authors and Affiliations

  • Jackson 715, G.I. Unit, Massachusetts General Hospital, Boston, USA

    Ian M. Rosenberg

Bibliographic Information

  • Book Title: Protein Analysis and Purification

  • Book Subtitle: Benchtop Techniques

  • Authors: Ian M. Rosenberg

  • DOI: https://doi.org/10.1007/978-1-4612-2056-5

  • Publisher: Birkhäuser Boston, MA

  • eBook Packages: Springer Book Archive

  • Copyright Information: Birkhäuser Boston 1996

  • Softcover ISBN: 978-0-8176-4341-6Published: 29 September 2004

  • eBook ISBN: 978-1-4612-2056-5Published: 01 December 2013

  • Edition Number: 2

  • Number of Pages: XXVI, 520

  • Topics: Protein Science, Biochemistry, general, Life Sciences, general

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