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Immunocytochemistry and In Situ Hybridization in the Biomedical Sciences

  • Book
  • © 2001

Overview

Editors:
  1. Julian E. Beesley

    1. Cell Biology, Glaxo Wellcome Medicines Research Centre, Stevenage, Hertfordshire, UK

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Table of contents (11 chapters)

  1. Front Matter

    Pages i-xi
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  2. Introduction

    • Julian E. Beesley
    Pages 1-5

  3. Preservation of Tissue for Immunocytochemical Studies

    • Iolo Ap Gwynn
    Pages 6-29

  4. Specimen Preparation for Immunocytochemistry

    • Gillian Brown, Anthony Wong, Julian E. Beesley
    Pages 30-54

  5. Antigen Retrieval

    • Peter Jackson, David Blythe
    Pages 55-72

  6. Immunolabeling

    • Alexander D. Hyatt, Terry G. Wise
    Pages 73-107

  7. In Situ Hybridization: General Principles and Application of Digoxigenin-Labeled cRNA for the Detection of mRNAs

    • Eveline Baumgart, Arno Schad, Markus Grabenbauer
    Pages 108-137

  8. Fluorescence In Situ Hybridization

    • Chee Gee See
    Pages 138-155

  9. In Situ Amplification and Detection of Nucleic Acids

    • Alfredo Martínez, Yan-gao Man, Steven J. Zullo, Frank Cuttitta
    Pages 156-174

  10. Image Analysis and Statistics: A Quantitative Approach to Immunocytochemistry and Related Techniques

    • Suzanna J. Newman, Paul Nelson
    Pages 175-199

  11. Safety in the Laboratory

    • Michael Hurle
    Pages 200-222

  12. Applications of Affinity Labeling in Biomedical Sciences

    • David A. Hughes
    Pages 223-253

  13. Back Matter

    Pages 255-267
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Keywords

About this book

Immunocytochemistry and in situ hybridization are widely used biomedical sciences. They are essential in medical diagnosis and in cell biology research. Affinity labeling is the central goal of the experimental strategy involving a series of techniques in a logical order; from the effects of specimen fixation, through specimen preparation to expose the antigen, to optimizing immunolabeling, to assessing the result and finally to safety considerations. Numerous examples of these techniques in biomedical sciences are included, as well as experimental assays and practical tips. This survey of methods will serve as an invaluable reference source in any laboratory setting (academic, industrial or clinical) involved in research in almost every branch of biology or medicine, as well as in pharmaceutical, biotechnological and clinical applications.

Editors and Affiliations

  • Cell Biology, Glaxo Wellcome Medicines Research Centre, Stevenage, Hertfordshire, UK

    Julian E. Beesley

Bibliographic Information

  • Book Title: Immunocytochemistry and In Situ Hybridization in the Biomedical Sciences

  • Editors: Julian E. Beesley

  • DOI: https://doi.org/10.1007/978-1-4612-0139-7

  • Publisher: Birkhäuser Boston, MA

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media New York 2001

  • Hardcover ISBN: 978-0-8176-4065-1Published: 12 October 2000

  • Softcover ISBN: 978-1-4612-6630-3Published: 17 May 2013

  • eBook ISBN: 978-1-4612-0139-7Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XI, 267

  • Topics: Cell Biology, Immunology, Life Sciences, general, Biochemistry, general

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