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Flow Cytometry in Microbiology

  • Book
  • © 1993

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Editors:
  1. David Lloyd

    1. University of Wales College of Cardiff, Cardiff, Wales

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Table of contents (14 chapters)

  1. Front Matter

    Pages i-xi
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  2. Flow Cytometry: A Technique Waiting for Microbiologists

    • David Lloyd
    Pages 1-9

  3. The Physical and Biological Basis for Flow Cytometry of Escherichia coli

    • Erik Boye, Harald B. Steen
    Pages 11-25

  4. Flow Cytometric Analysis of Heterogeneous Bacterial Populations

    • Richard Allman, Richard Manchee, David Lloyd
    Pages 27-47

  5. On the Determination of the Size of Microbial Cells Using Flow Cytometry

    • Hazel M. Davey, Chris L. Davey, Douglas B. Kell
    Pages 49-65

  6. Uses of Membrane Potential Sensitive Dyes with Bacteria

    • David Mason, Richard Allman, David Lloyd
    Pages 67-81

  7. Flow Cytometric Analysis, Using Rhodamine 123, of Micrococcus luteus at Low Growth Rate in Chemostat Culture

    • Hazel M. Davey, Arseny S. Kaprelyants, Douglas B. Kell
    Pages 83-93

  8. Characterization of Bacterial Cell Size and Ploidy using Flow Cytometry and Image Analysis

    • Jerome Durodie, Kenneth Coleman, Michael J. Wilkinson
    Pages 95-109

  9. Cytometric Evaluation of Antifungal Agents

    • Elizabeth A. Carter, Frank E. Paul, Pamela A. Hunter
    Pages 111-120

  10. Applications of Flow Cytometry in Bacterial Ecology

    • Clive Edwards, Julian P. Diaper, Jonathan Porter, Roger Pickup
    Pages 121-129

  11. Analysis of Microalgae and Cyanobacteria by Flow Cytometry

    • Alex Cunningham
    Pages 131-142

  12. Flow Cytometry at Sea

    • Glen A. Tarran, Peter H. Burkill
    Pages 143-158

  13. Neural Network Analysis of Flow Cytometry Data

    • Lynne Boddy, Colin W. Morris
    Pages 159-169

  14. Rapid Analysis of Microorganisms Using Flow Cytometry

    • Michael Brailsford, Stephen Gatley
    Pages 171-180

  15. Appendix: Spectral Characteristics of Some Fluorescent Dyes and Excitation Sources

    • David Lloyd
    Pages 181-183

  16. Back Matter

    Pages 185-188
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Keywords

About this book

As yet, flow cytometry is not used so widely in microbiology as in some other disciplines. This volume presents contributions flow cytometry to study a from research microbiologists who use diverse set of problems. It illustrates the power of the technique, and may persuade others of its usefulness. Most of the con­ tributors gathered in Cardiff on 23 October 1991, at a meeting organized for the Royal Microscopical Society by Dr. Richard Allman, but the content of their chapters is not limited by the discourse of that meeting, and for balance other experts were invited to write for this book. Flow Cytometry in Microbiology thus represents the first collection of articles specifically devoted to the applications of a technique which promises so much to those investigating the microbial world. Cardiff, 1992 David Lloyd Contents List of Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix 1 Flow Cytometry: A Technique Waiting for Microbiologists David Lloyd . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 2 The Physical and Biological Basis for Flow Cytometry of Escherichia coli Erik Boye and Harald B. Steen . . . . . . . . . . . . . . . . . . . . . . 11 3 Flow Cytometric Analysis of Heterogeneous Bacterial Populations Richard Allman, Richard Manchee and David Lloyd. . . . 27 4 On the Determination of the Size of Microbial Cells Using Flow Cytometry Hazel M. Davey, Chris L. Davey and Douglas B. Kell . . 49 5 Uses of Membrane Potential Sensitive Dyes with Bacteria David Mason, Richard Allman and David Lloyd . . . . . . .

Editors and Affiliations

  • University of Wales College of Cardiff, Cardiff, Wales

    David Lloyd

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