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  • © 2011

Biophysical Chemistry of Proteins

An Introduction to Laboratory Methods

Authors:

  • Focuses on the biophysical chemistry of proteins

  • Presents an overview of the methods used in protein research

  • Examines the possible applications and limitations of protein research

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Table of contents (53 chapters)

  1. Front Matter

    Pages i-xvi
  2. Analytical Techniques

    1. Front Matter

      Pages 1-1
    2. Microscopy

      • Engelbert Buxbaum
      Pages 3-22
    3. Single Molecule Techniques

      • Engelbert Buxbaum
      Pages 23-24
    4. Preparation of Cells and Tissues for Microscopy

      • Engelbert Buxbaum
      Pages 25-26
    5. Principles of Optical Spectroscopy

      • Engelbert Buxbaum
      Pages 27-31
    6. Photometry

      • Engelbert Buxbaum
      Pages 33-37
    7. Fluorimetry

      • Engelbert Buxbaum
      Pages 39-55
    8. Chemiluminescence

      • Engelbert Buxbaum
      Pages 57-60
    9. Electrophoresis

      • Engelbert Buxbaum
      Pages 61-95
    10. Immunological Methods

      • Engelbert Buxbaum
      Pages 97-122
    11. Isotope Techniques

      • Engelbert Buxbaum
      Pages 123-132
  3. Purification of Proteins

    1. Front Matter

      Pages 133-133
    2. Isolation of Organelles

      • Engelbert Buxbaum
      Pages 141-141
    3. Precipitation Methods

      • Engelbert Buxbaum
      Pages 143-146
    4. Chromatography

      • Engelbert Buxbaum
      Pages 147-162
    5. Membrane Proteins

      • Engelbert Buxbaum
      Pages 165-182
    6. Determination of Protein Concentration

      • Engelbert Buxbaum
      Pages 183-186
    7. Cell Culture

      • Engelbert Buxbaum
      Pages 187-189

About this book

The book is structured in nine sections, each containing several chapters. The volume starts with an overview of analytical techniques and progresses through purification of proteins; protein modification and inactivation; protein size, shape, and structure; enzyme kinetics; protein-ligand interactions; industrial enzymology; and laboratory quality control. The book is targeted at all scientists interested in protein research.

Authors and Affiliations

  • P.O. Box 266, Biochemistry, Ross University School of Medicine, Roseau, Dominica

    Engelbert Buxbaum

About the author

Dr. Buxbaum's research interests are enzymology and protein structure/function relationship. In addition, he has been teaching science and medical students in several countries. He is currently working as associate professor of biochemistry at Ross University School of Medicine.

Bibliographic Information

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access