Working with Ferns

1. Front Matter

   Pages i-xxii

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2. Introduction

   • H. Fernández

   Pages 1-8

3. Contribution of Ferns to Understanding of Plant Developme

   1. Front Matter

      Pages 9-9

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4. CONTRIBUTION OF FERNS TO UNDERSTANDING OF PLANT DEVELOPMENT

   1. Cellular, Molecular, and Genetic Changes During the Development of Ceratopteris richardii Gametophytes

      • Mari L. Salmi, Thomas Bushart, Stanley J. Roux

      Pages 11-24

   2. Laboratory-Induced Apogamy and Apospory in Ceratopteris richardii

      • Angela R. Cordle, Linh Thuy Bui, Erin E. Irish, Chi-Lien Cheng

      Pages 25-36

   3. Sexual Reproduction in Ferns

      • V. Menéndez, E. Peredo, M. Méndez, A. Revilla, H. Fernández

      Pages 37-48

   4. Gibberellic Acid and Ethylene Control Male Sex Determination and Development of Anemia phyllitidis Gametophytes

      • Andrzej Kaźmierczak

      Pages 49-65

   5. The Sporophytes of Seed-Free Vascular Plants – Major Vegetative Developmental Features and Molecular Genetic Pathways

      • Alexandru M. F. Tomescu

      Pages 67-94

5. Propagation, Conservation and Control of Genetic Variability in Ferns

   1. Front Matter

      Pages 95-95

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6. PROPAGATION, CONSERVATION AND CONTROL OF GENETIC VARIABILITY IN FERNS

   1. From Spore to Sporophyte: How to Proceed In Vitro

      • V. Menéndez, R. Arbesú, M. Somer, A. Revilla, H. Fernández

      Pages 97-110

   2. In Vitro Regeneration Systems of Platycerium

      • Marjana Camloh, Jana Ambrožič-Dolinšek

      Pages 111-125

   3. Stipule Propagation in Five Marattioid Species Native to Taiwan (Marattiaceae; Pteridophyte)

      • Yao-Moan Huang, Mei-Hsueh Huang, Chun-Ming Chen, Wen-Liang Chiou

      Pages 127-134

   4. Tree Ferns Biotechnology: From Spores to Sporophytes

      • Jan J. Rybczyński, Anna Mikuła

      Pages 135-147

   5. In Vitro Propagation of Rare and Endangered Serpentine Fern Species

      • Jowita Marszał-Jagacka, Krystyna Kromer

      Pages 149-164

   6. Conservation of Fern Spores

      • Daniel Ballesteros

      Pages 165-172

   7. Exploration of Cryo-methods to Preserve Tree and Herbaceous Fern Gametophytes

      • Anna Mikuła, Damian Makowski, Christina Walters, Jan J. Rybczyński

      Pages 173-192

   8. Pteridophyte spores viability

      • Jose María Gabriel y Galán, Carmen Prada

      Pages 193-205

   9. Microsatellites: A Powerful Genetic Marker for Fern Research

      • Ares Jiménez

      Pages 207-220

   10. Diversity in Natural Fern Populations: Dominant Markers as Genetic Tools

       • E. L. Peredo, A. Revilla, M. Méndez, V. Menéndez, H. Fernández

       Pages 221-234

7. Environmental Biotechnology: Ecotoxicology and Bioremediation in Ferns

   1. Front Matter

      Pages 235-235

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Ferns existing today represent a genetic inheritance of great value as they include species of ancient vascular plants, which have direct connection with the crucial steps done in the past for settling life on Earth. Their life cycle is an example of alternation of generations, in which both a multicellular diploid organism and a multicellular haploid organism occur and give rise to the other. Spore, gametophyte and sporophyte have been studied either for basic and practical purposes. In the first case, they offer us simple and easy to grow experimental systems to investigate on plant development, and secondly, they are culture by their ornamental appeal, their environmental benefit or as source of metabolites. This timely volume brings a selection of chapters, each one composed by experts in their respective fields. The chapters included cover a broad range from the knowledge of its biology and contribution to understanding of plant development, useful protocols for propagation and conservation purposes, genetic variability as well as environmental and theurapeutical applications. This wide spectrum of the contributions gives to reader a rapid idea of the enormous potential of this plant group. The originality of this book is to expose the most recent tendencies in their investigation, which is far from the traditional perspective usually followed. The collected articles in this volume incorporate most of novel techniques used nowadays routinely to resolve traditional questions. Ashwani Kumar Professor, Department of Botany, University of Rajasthan, Jaipur. The author’s repertoire of published works spreads across 150 research articles in various national and international journals. He has an experience of over three decades in his field of research, namely, tissue culture and biochemistry, being awarded with prestigeous V. Puri Medal in 2008 for his services to advancement of Botany. Helena Fernández Associate Professor, Department of Biology of Organisms and Systems, Oviedo University, Spain. Her research focuses on micropropagation and reproduction in ferns during the two last decades. In 2002 she obtained the award “Ramón y Cajal” by The Ministry of Science and Technology, being engaged to the Oviedo University as tenurer, full time, in the Area of Plant Physiology since then. M. Angeles Revilla Plant Physioloy Assistant Professor in the Biology Faculty at the Oviedo University (Spain) since 1987. Twenty years experience in plant tissue culture. She has also worked in cryopreservation and genetic stability for the last ten years, mainly in the development of protocols for in vitro shoot apices in agronomic species.

• Ferns
• Plant Science
• Pteridophyta
• Seed
• Spore
• biotechnology
• plant development

• Dept. Botany, University of Rajasthan, Jaipur, India

  Ashwani Kumar

• Depto. Biologia de Organismos y Sis, Unidad de Fisologia Vegetal, Universidad de Oviedo, Oviedo, Spain

  Helena Fernández

• , Dpt. Biología de Organismos y Sistemas, Universidad de Oviedo, Oviedo, Spain

  Maria Angeles Revilla

Ashwani Kumar Professor, Department of Botany, University of Rajasthan, Jaipur. The author’s repertoire of published works spreads across 150 research articles in various national and international journals. He has an experience of over three decades in his field of research, namely, tissue culture and biochemistry, being awarded with prestigeous V. Puri Medal in 2008 for his services to advancement of Botany. Helena Fernández Associate Professor, Department of Biology of Organisms and Systems, Oviedo University, Spain. Her research focuses on micropropagation and reproduction in ferns during the two last decades. In 2002 she obtained the award “Ramón y Cajal” by The Ministry of Science and Technology, being engaged to the Oviedo University as tenurer, full time, in the Area of Plant Physiology since then. M. Angeles Revilla Plant Physioloy Assistant Professor in the Biology Faculty at the Oviedo University (Spain) since 1987. Twenty years experience in plant tissue culture. She has also worked in cryopreservation and genetic stability for the last ten years, mainly in the development of protocols for in vitro shoot apices in agronomic species.

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