The Cell Biology of Stem Cells

1. Front Matter

   Pages i-xx

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2. Early Embryonic Cell Fate Decisions in the Mouse

   • Yojiro Yamanaka, Amy Ralston

   Pages 1-13

3. Nuclear Architecture in Stem Cells

   • Kelly J. Morris, Mita Chotalia, Ana Pombo

   Pages 14-25

4. Epigenetic Regulation of Pluripotency

   • Eleni M. Tomazou, Alexander Meissner

   Pages 26-40

5. Autosomal Lyonization of Replication Domains During Early Mammalian Development

   • Ichiro Hiratani, David M. Gilbert

   Pages 41-58

6. Preservation of Genomic Integrity in Mouse Embryonic Stem Cells

   • Peter J. Stambrook, Elisia D. Tichy

   Pages 59-75

7. Transcriptional Regulation in Embryonic Stem Cells

   • Jian-Chien Dominic Heng, Huck-Hui Ng

   Pages 76-91

8. Alternative Splicing in Stem Cell Self-Renewal and Diferentiation

   • David A. Nelles, Gene W. Yeo

   Pages 92-104

9. MicroRNA Regulation of Embryonic Stem Cell Self-Renewal and Differentiation

   • Collin Melton, Robert Blelloch

   Pages 105-117

10. Telomeres And Telomerase in Adult Stem Cells and Pluripotent Embryonic Stem Cells

    • Rosa M. Marión, Maria A. Blasco

    Pages 118-131

11. X Chromosome Inactivation and Embryonic Stem Cells

    • Tahsin Stefan Barakat, Joost Gribnau

    Pages 132-154

12. Adult Stem Cels and Their Niches

    • Francesca Ferraro, Cristina Lo Celso, David Scadden

    Pages 155-168

13. Adult Stem Cel Diferentiation and Trafficking And Their Implications in Disease

    • Ying Zhuge, Zhao-Jun Liu, Omaida C. Velazquez

    Pages 169-183

14. Vertebrates That Regenerate As Models For Guiding Stem Cels

    • Christopher L. Antos, Elly M. Tanaka

    Pages 184-214

15. Reprogramming of Somatic Cells to Pluripotency

    • Masato Nakagawa, Shinya Yamanaka

    Pages 215-224

16. Back Matter

    Pages 225-229

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Stem cells have been gaining a lot of attention in recent years. Their unique potential to self-renew and differentiate has turned them into an attractive model for the study of basic biological questions such as cell division, replication, transcription, cell fate decisions, and more. With embryonic stem (ES) cells that can generate each cell type in the mammalian body and adult stem cells that are able to give rise to the cells within a given lineage, basic questions at different developmental stages can be addressed. Importantly, both adult and embryonic stem cells provide an excellent tool for cell therapy, making stem cell research ever more pertinent to regenerative medicine. As the title The Cell Biology of Stem Cells suggests, our book deals with multiple aspects of stem cell biology, ranging from their basic molecular characteristics to the in vivo stem cell trafficking of adult stem cells and the adult stem-cell niche, and ends with a visit to regeneration and cell fate reprogramming. In the first chapter, “Early embryonic cell fate decisions in the mouse”, Amy Ralson and Yojiro Yamanaka describe the mechanisms that support early developmental decisions in the mouse pre-implantation embryo and the current understanding of the source of the most immature stem cell types, which includes ES cells, trophoblast stem (TS) cells and extraembryonic endoderm stem (XEN) cells.

• Activation
• Chromosome
• Telomere
• Vivo
• evolution
• genes
• miRNAs
• regulation

• Department of Genetics, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel

  Eran Meshorer

• Department of Biological Chemistry, UCLA School of Medicine, Los Angeles, USA

  Kathrin Plath

Eran Mes horer, PhD, is studying chromatin plasticity in embryonic and neuronal stem cells at the Department of Genetics at the Hebrew University of Jerusalem. He received his PhD in Molecular Neuroscience from the Hebrew University and conducted his post‑doctoral studies at the National Cancer Institute, NIH. His lab focuses on understanding pluripotency, differentiation and reprogramming from a chromatin perspective, taking both genome‑wide and single cell approaches. He is a member of the International Society for Stem Cell Research and holds the Joseph H. and Belle R. Braun Senior Lectureship in Life Sciences. Kathrin Plath, PhD, is an Assistant Professor in the Department of Biological Chemistry at the University of California Los Angeles since 2004. After she received her PhD from the Humboldt University at Berlin in Germany, she was at the University of California San Francisco and the Whitehead Institute in Cambridge, MA for her postdoctoral studies. Dr. Plath’s main research interest is to understand how developmental cues induce changes in chromatin structure at the molecular level, and how these changes regulate cell fate decisions and gene expression in mammalian development. She is a member of the International Society for Stem Cell Research and of the editorial board of several stem cell journals.

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