Single-Channel Recording

1. Front Matter

   Pages i-xxvii

   PDF

2. Introduction to Patch Clamping

   1. Front Matter

      Pages 1-1

      PDF

   2. A Practical Guide to Patch Clamping

      • Reinhold Penner

      Pages 3-30

   3. Tight-Seal Whole-Cell Recording

      • Alain Marty, Erwin Neher

      Pages 31-52

   4. Guide to Data Acquisition and Analysis

      • Stefan H. Heinemann

      Pages 53-91

3. Methods

   1. Front Matter

      Pages 93-93

      PDF

   2. Electronic Design of the Patch Clamp

      • F. J. Sigworth

      Pages 95-127

   3. Low-Noise Recording

      • Klaus Benndorf

      Pages 129-145

   4. Voltage Offsets in Patch-Clamp Experiments

      • Erwin Neher

      Pages 147-153

   5. Techniques for Membrane Capacitance Measurements

      • Kevin D. Gillis

      Pages 155-198

   6. Patch-Pipette Recordings from the Soma, Dendrites, and Axon of Neurons in Brain Slices

      • Bert Sakmann, Greg Stuart

      Pages 199-211

   7. Patch Clamp and Calcium Imaging in Brain Slices

      • Jens Eilers, Ralf Schneggenburger, Arthur Konnerth

      Pages 213-229

   8. Fast Application of Agonists to Isolated Membrane Patches

      • Peter Jonas

      Pages 231-243

   9. Electrochemical Detection of Secretion from Single Cells

      • Robert H. Chow, Ludolf Von Rüden

      Pages 245-275

   10. Technical Approaches to Studying Specific Properties of Ion Channels in Plants

       • Rainer Hedrich

       Pages 277-305

   11. The Giant Membrane Patch

       • Donald W. Hilgemann

       Pages 307-327

   12. A Fast Pressure-Clamp Technique for Studying Mechanogated Channels

       • Don W. McBride Jr., Owen P. Hamill

       Pages 329-340

   13. Electrophysiological Recordings from Xenopus Oocytes

       • Walter Stühmer, Anant B. Parekh

       Pages 341-356

   14. Polymerase Chain Reaction Analysis of Ion Channel Expression in Single Neurons of Brain Slices

       • Hannah Monyer, Peter Jonas

       Pages 357-373

   15. Force Microscopy on Membrane Patches

       • J. K. Heinrich Hörber, Johannes Mosbacher, Walter Häberle

       Pages 375-393

Edited by the 1991 winners of the Nobel Prize in Physiology or Medicine, this Second Edition includes new chapters covering such applications as capacitance measurements; single-cell PCR measurements; whole-cell recording from brain slices in combination with imaging techniques; atomic force microscopy of cells and membranes attached to glass pipettes; and patch clamping.

• cells
• channel
• imaging
• imaging techniques
• microscopy
• neher
• neurons
• physiology
• recording
• sakmann
• single
• single-channel
• soft
• soft cover

From reviews of the First Edition:

"A landmark in the field of membrane biophysics." (Science)

"A first-class laboratory guide...an indispensable reference." (Neuroscience)

• Department of Cell Physiology, Max-Planck-Institute for Medical Research, Heidelberg, Germany

  Bert Sakmann

• Department of Membrane Biophysics, Max-Planck-Institute for Biophysical Chemistry, Göttingen, Germany

  Erwin Neher

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