Protein Hydrolysates in Biotechnology

1. Front Matter

   Pages i-xiii

   PDF

2. Applications of Protein Hydrolysates in Biotechnology

   • Vijai K. Pasupuleti, Chris Holmes, Arnold L. Demain

   Pages 1-9

3. State of the Art Manufacturing of Protein Hydrolysates

   • Vijai K. Pasupuleti, Steven Braun

   Pages 11-32

4. Towards an Understanding of How Protein Hydrolysates Stimulate More Efficient Biosynthesis in Cultured Cells

   • André Siemensma, James Babcock, Chris Wilcox, Hans Huttinga

   Pages 33-54

5. Benefits and Limitations of Protein Hydrolysates as Components of Serum-Free Media for Animal Cell Culture Applications

   • Juliet Lobo-Alfonso, Paul Price, David Jayme

   Pages 55-78

6. Oligopeptides as External Molecular Signals Affecting Growth and Death in Animal Cell Cultures

   • František Franek

   Pages 79-89

7. Use of Protein Hydrolysates in Industrial Starter Culture Fermentations

   • Madhavi (Soni) Ummadi, Mirjana Curic-Bawden

   Pages 91-114

8. Protein Hydrolysates from Non-bovine and Plant Sources Replaces Tryptone in Microbiological Media

   • Yamini Ranganathan, Shifa Patel, Vijai K. Pasupuleti, R. Meganathan

   Pages 115-125

9. The Use of Protein Hydrolysates for Weed Control

   • Nick Christians, Dianna Liu, Jay Bryan Unruh

   Pages 127-133

10. Physiological Importance and Mechanisms of Protein Hydrolysate Absorption

    • Brian M. Zhanghi, James C. Matthews

    Pages 135-177

11. Protein Hydrolysates/Peptides in Animal Nutrition

    • Jeff McCalla, Terry Waugh, Eric Lohry

    Pages 179-190

12. Protein Hydrolysates as Hypoallergenic, Flavors and Palatants for Companion Animals

    • Tilak W. Nagodawithana, Lynn Nelles, Nayan B. Trivedi

    Pages 191-207

13. The Development of Novel Recombinant Human Gelatins as Replacements for Animal-Derived Gelatin in Pharmaceutical Applications

    • David Olsen, Robert Chang, Kim E. Williams, James W. Polarek

    Pages 209-225

14. Back Matter

    Pages 227-229

    PDF

Protein hydrolysates, otherwise commonly known as peptones or peptides, are used in a wide variety of products in fermentation and biotechnology industries. The term “peptone” was first introduced in 1880 by Nagelli for growing bacterial cultures. However, later it was discovered that peptones derived from the partial digestion of proteins would furnish organic nitrogen in readily available form. Ever since, p- tones, which are commonly known as protein hydrolysates, have been used not only for growth of microbial cultures, but also as nitrogen source in commercial fermen- tions using animal cells and recombinant microorganisms for the production of value added products such as therapeutic proteins, hormones, vaccines, etc. Today, the characterization, screening and manufacturing of protein hyd- lysates has become more sophisticated, with the introduction of reliable analytical instrumentation, high throughput screening techniques coupled with statistical design approaches, novel enzymes and efficient downstream processing equipment. This has enabled the introduction of custom-built products for specialized appli- tions in diverse fields of fermentation and biotechnology, such as the following. 1. Protein hydrolysates are used as much more than a simple nitrogen source. For example, the productivities of several therapeutic drugs made by animal cells and recombinant microorganisms have been markedly increased by use of p- tein hydrolysates. This is extremely important when capacities are limited. 2. Protein hydrolysates are employed in the manufacturing of vaccines by ferm- tation processes and also used as vaccine stabilizers.

• Animal Cell Culture
• Fermentation
• Fermentations
• Nutraceuticals
• Peptones
• Protein Hydrolysates
• biotechnology
• nitrogen

• SAI International, Inc., Geneva, USA

  Vijai K. Pasupuleti

• Charles A. Dana Resarch Inst., Scientists Emeriti (RISE), Drew University, Madison, USA

  Arnold L. Demain

Policies and ethics