Skip to main content
SpringerLink
Log in

Evolving Methods for Macromolecular Crystallography

The Structural Path to the Understanding of the Mechanism of Action of CBRN Agents

  • Conference proceedings
  • © 2007

Overview

Editors:
  1. Randy J. Read

    1. University of Cambridge, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Joel L. Sussman

    1. Weizmann Institute of Science, Israel

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Arises from the renowned Erice Schools on Macromolecular Crystallography
  • Chapters by leading experts in modern methods of macromolecular crystallography
  • Spans the range from detailed practical advice to advanced analytical techniques

Part of the book series: NATO Science Series II: Mathematics, Physics and Chemistry (NAII, volume 245)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (15 papers)

  1. Front Matter

    Pages i-viii
    Download chapter PDF

  2. Succeeding with seeding: some practical advice

    • Terese Bergfors
    Pages 1-10

  3. Expression, purification, and crystallisationof membrane proteins

    • Bernadette Byrne
    Pages 11-23

  4. Macromolecular cryo-crystallography

    • Elspeth Garman
    Pages 25-40

  5. Processing diffraction data with mosflm

    • Andrew G. W. Leslie, Harold R. Powell
    Pages 41-51

  6. Sad phasing: basic concepts and high-throughput

    • George M. Sheldrick
    Pages 53-65

  7. Likelihood-based experimental phasing in phaser

    • Airlie J. McCoy, Laurent C. Storoni, Randy J. Read
    Pages 67-77

  8. Stochastic molecular replacement

    • Nicholas M. Glykos
    Pages 79-90

  9. Likelihood-based molecular replacement in phaser

    • Randy J. Read, Airlie J. McCoy, Laurent C. Storoni
    Pages 91-100

  10. Automated structure determination with phenix

    • Tod D. Romo, James C. Sacchettini, Thomas C. Terwilliger, Paul D. Adams, Pavel V. Afonine, Ralf W. Grosse-Kunstleve et al.
    Pages 101-109

  11. Density Modification In Main

    • Dušan Turk
    Pages 111-122

  12. Ab initio phasing starting from low resolution

    • Vladimir Lunin, Natalia Lunina, Alexandre Urzhumtsev
    Pages 123-133

  13. Structural genomics of mycobacterium tuberculosis: a search for function and new drug targets

    • Ted Baker
    Pages 135-144

  14. Three-dimensional domain swapping and its relevance to conformational diseases

    • Mariusz Jaskolski
    Pages 145-163

  15. Structural bioinformatics: from protein structure to function

    • Aled M. Edwards, James D. Watson, Adel Golovin, Roman A. Laskowski, Kim Henrick, Janet M. Thornton et al.
    Pages 165-179

  16. Single-particle imaging

    • David Sayre
    Pages 181-191
Back to top

Keywords

About this book

This volume comprises papers presented at the 2005 edition of the “Crystallography of Molecular Biology” courses that have been held since 1976 at the Ettore Majorana Centre for Scientific Culture in Erice, Italy. This series of courses is renowned for bringing leaders in the field of macromo- cular crystallography together with highly motivated students, in a beautiful and intimate location that encourages people to interact. The warm and informal atmosphere at these Erice conferences, especially these on cryst- lography, has helped to foster long-term scientific interactions and new int- national friendships that have often lasted for the lifetime of the scientists. The course was financed by NATO as an Advanced Study Institute and by the European Commission as a EuroSummerSchool. The papers span the breadth of material presented in the course, which emphasized the practical aspects of modern macromolecular crystallography and its applications. One must start with crystals: Bergfors showed how to improve initial crystals through seeding, while Byrne discussed the difficult problem of crystallizing membrane proteins. The collection of optimal diffraction data requires both careful preparation of cryo-cooled crystals (Garman) and proper processing of the diffraction images (Leslie). To obtain images of electron density, one needs estimates of the phases of the diffracted spots. Sheldrick presented the background to the sing- wavelength anomalous diffraction (SAD) method, which has been gaining popularity, and McCoy discussed the basis of modern maximum likelihood methods for treating information in experimental phasing.

Reviews

From the reviews:

"The book is a summary of the proceedings on ‘Evolving Methods for Macromolecular Crystallography’ held in 2005. … it is a welcome addition to the crystallographer’s bookshelf with contributions by a number of experts in their field." (Miroslav Papiz, Crystallography Reviews, Vol. 15 (2), 2009)

Editors and Affiliations

  • University of Cambridge, UK

    Randy J. Read

  • Weizmann Institute of Science, Israel

    Joel L. Sussman

Bibliographic Information

Publish with us

Policies and ethics

Back to top

Search

Navigation