Yeast as a Tool in Cancer Research

1. Front Matter

   Pages i-xvi

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2. From DNA Replication to Genome Instability in Schizosaccharomyces Pombe: Pathways to Cancer

   • Julie M. Bailis, Susan L. Forsburg

   Pages 1-35

3. Dissecting Layers of Mitotic Regulation Essential for Maintaining Genomic Stability

   • Jennifer S. Searle, Yolanda Sanchez

   Pages 37-73

4. Yeast as a Tool in Cancer Research: Nuclear Trafficking

   • Anita H. Corbett, Adam C. Berger

   Pages 75-100

5. Studies of Protein Farnesylation in Yeast

   • Nitika Thapar, Fuyuhiko Tamanoi

   Pages 101-122

6. From Bread to Bedside: What Budding Yeast has Taught us about the Immortalization of Cancer Cells

   • Soma S. R. Banik, Christopher M. Counter

   Pages 123-139

7. HSP90 Co-Chaperones in Saccharomyces Cerevisiae

   • Marija Tesic, Richard F. Gaber

   Pages 141-177

8. Yeast as a Model System for Studying Cell Cycle Checkpoints

   • Carmela Palermo, Nancy C. Walworth

   Pages 179-189

9. Metabolism and Function of Sphingolipids in Saccharomyces Cerevisiae: Relevance to Cancer Research

   • L. Ashely Cowart, Yusuf A. Hannun, Lina M. Obeid

   Pages 191-210

10. Exploring and Restoring the p53 Pathway Using the p53 Dissociator Assay in Yeast

    • Rainer K. Brachmann

    Pages 211-232

11. Functional Analysis of the Human p53 Tumor Suppressor and its Mutants Using Yeast

    • Alberto Inga, Francesca Storici, Michael A. Resnick

    Pages 233-288

12. ABC Transporters in Yeast – Drug Resistance and Stress Response in a Nutshell

    • Karl Kuchler, Christoph Schüller

    Pages 289-314

13. The FHCRC/NCI Yeast Anticancer Drug Screen

    • Susan L. Holbeck, Julian Simon

    Pages 315-346

14. Yeast as a Model to Study the Immunosuppressive and Chemotherapeutic Drug Rapamycin

    • John R. Rohde, Sara A. Zurita-Martinez, Maria E. Cardenas

    Pages 347-374

15. Use of Yeast as a Model System for Identifying and Studying Anticancer Drugs

    • Jun O. Liu, Julian A. Simon

    Pages 375-391

16. Genetic Analysis of Cisplatin Resistance in Yeast and Mammals

    • Seiko Ishida, Ira Herskowitz

    Pages 393-408

17. Using Yeast Tools to Dissect the Action of Anticancer Drugs: Mechanisms of Enzyme Inhibition and Cell Killing by Agents Targeting DNA Topoisomerases

    • Anna T. Rogojina, Zhengsheng Li, Karin C. Nitiss, John L. Nitiss

    Pages 409-427

18. Back Matter

    Pages 429-433

    PDF

Leland H. Hartwell Director, Fred Hutchinson Cancer Research Center, Nobel Laureate for Medicine, 2001 Yeast has proved to be the most useful single-celled organism for studying the fundamental aspects of cell biology. Resources are now available for yeast that greatly simplify and empower new investigations, like the presence of strains with each gene deleted, each protein tagged and databases on protein–protein interactions, gene regulation, and subcellular protein location. A powerful combination of genetics, cell biology, and biochemistry employed by thousands of yeast researchers has unraveled the complexities of numerous cellular processes from mitosis to secretion and even uncovered new insights into prion diseases and the role of prions in normal biology. These insights have proven, time and again, to foretell the roles of proteins and pathways in human cells. The collection of articles in this volume explores the use of yeast in pathway analysis and drug discovery. Yeast has, of course, supplied mankind’s most ubiquitous drug for thousands of years. In one aspect, the role of yeast in drug discovery is much like the role of yeast in other areas of biology. Yeast offers the power of genetics and a repetoire of resources available in no other organism. Using yeast in the study of drug targets and metabolism can help to make a science of what has been largely an empirical activity. A science of drug discovery would permit rigorous answers to important questions.

• Mycin
• Radiologieinformationssystem
• cancer
• drug discovery
• metabolism
• protein
• transport

• St. Jude Children's Research Hospital, Memphis, USA

  John L. Nitiss

• Duke University Medical Center, Durham, USA

  Joseph Heitman

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