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Reviews and Protocols in DT40 Research

Subcellular Biochemistry

  • Book
  • © 2006

Overview

Editors:
  1. Jean-Marie Buerstedde

    1. GSF National Research Centre, Neuherberg, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Shunichi Takeda

    1. Kyoto University, Japan

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    You can also search for this editor in PubMed   Google Scholar

  • State-of-the-art reviews
  • Protocols to help you succeed
  • Frontiers in DT40 science

Part of the book series: Subcellular Biochemistry (SCBI, volume 40)

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Table of contents (43 chapters)

  1. Front Matter

    Pages i-xii
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  2. DT40 gene disruptions: a how-to for the design and the construction of targeting vectors

    • Hiroshi Arakawa, Jean-Marie Buerstedde
    Pages 1-9

  3. Immunoglobulin gene conversion or hypermutation: that's the question

    • Jean-Marie Buerstedde, Hiroshi Arakawa
    Pages 11-24

  4. Genome resources for the DT40 community

    • Randolph B. Caldwell, Andrzej M. Kierzek
    Pages 25-37

  5. Chromosome engineering in DT40 cells and mammalian centromere function

    • William R. A. Brown, Margaret C. M. Smith, Felix Dafhnis-Calas, Sunir Malla, Zhengyao Xu
    Pages 39-48

  6. Function of RECQ family helicase in genome stability

    • Masayuki Seki, Shusuke Tada, Takemi Enomoto
    Pages 49-73

  7. Genetic analysis of apoptotic execution

    • Sandrine Ruchaud, Kumiko Samejima, Damien Hudson, Scott H. Kaufmann, William C. Earnshaw
    Pages 75-90

  8. The DT40 system as a tool for analyzing kinetochore assembly

    • Masahiro Okada, Tetsuya Hori, Tatsuo Fukagawa
    Pages 91-106

  9. Analysing the DNA damage and replication checkpoints in DT40 cells

    • Michael D. Rainey, George Zachos, David A. F. Gillespie
    Pages 107-117

  10. Using DT40 to study clathrin function

    • Frank R. Wettey, Antony P. Jackson
    Pages 119-143

  11. Genetic analysis of B cell signaling

    • Hisaaki Shinohara, Tomohiro Kurosaki
    Pages 145-187

  12. DT40 mutants: a model to study transcriptional regulation of B cell development and function

    • Jukka Alinikula, Olli Lassila, Kalle-Pekka Nera
    Pages 189-205

  13. Transcription and RNA processing factors play complex roles in DT40 cells

    • Stephanie Bush, James L. Manley
    Pages 207-223

  14. Participation of histones, histone modifying enzymes and histone chaperonesin vertebrate cell functions

    • Hidehiko Kikuchi, Hirak Kumar Barman, Masami Nakayama, Yasunari Takami, Tatsuo Nakayama
    Pages 225-243

  15. Analysis of gene expression, copy number and palindrome formation with a DT40 enriched CDNA microarray

    • Paul E. Neima, Joan Burnside, Katrina Elsaesser, Harry Hwang, Bruce E. Clurman, Robert Kimmel et al.
    Pages 245-256

  16. Calcium signaling, ion channels and more

    • Anne-Laure Perraud, Carsten Schmitz, Andrew M. Scharenberg
    Pages 257-270

  17. Analysis of DNA replication damage bypass and its role in immunoglobulin repertoire development

    • Julian E. Sale, Anna-Laura Ross, Laura J. Simpson
    Pages 271-294

  18. The fanconi anemia pathway promotes homologous recombination repair in DT40 cell line

    • Minoru Takata, Kazuhiko Yamamoto, Nobuko Matsushita, Hiroyoki Kitao, Sciki Hirano, Masamichi Ishaiai
    Pages 295-311

  19. Phenotypic analysis of cellular responses to DNA damage

    • Helfrid Hochegger, Shunichu Takeda
    Pages 313-325

  20. ATM, a paradigm for a stress-responsive signal transducer in higher vertebrate cells

    • Ken-ichi Yamamoto, Masahiko Kobayashi, Hiroko Shimizu
    Pages 327-339
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Keywords

About this book

Research is fast paced and advances in RNA interference have recently opened up new opportunities for genetic experiments in human cell lines. However, the possibility to easily modify the genome still remains a powerful tool to investigate the function of coding and regulatory sequences in the vertebrate genome. DT40 has never been a quick and easy road to fame. It would be unfair to blame this on DT40 as it has proven to be a reliable and robust companion with fast doubling time, easy clonability and a relatively stable karyotype. If this model system is going to flourish over the next 15 years, it will be thanks to ingenious and original researchers. They may feel as if they work outside the mainstream, but they can take heart by the fact that only the clever exploitation of diversity and conservation makes biological research both elegant and rewarding. It is with this in mind that the DT40 handbook has been conceived and written. This book provides an up to date overview of the different facets of research, and also intends to help newcomers get started and avoid looming pitfalls. The collection of protocols which have been kindly provided by a number of laboratories will be particularly useful in this regard.

Editors and Affiliations

  • GSF National Research Centre, Neuherberg, Germany

    Jean-Marie Buerstedde

  • Kyoto University, Japan

    Shunichi Takeda

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