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Transgenic Crops of the World

Essential Protocols

  • Book
  • © 2004

Overview

Editors:
  1. Ian S. Curtis

    1. Department of Biotechnology, National Institute of Agrobiological Sciences (NIAS), Ibaraki, Japan

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  • Is a state-of-the-art, compilation of the most up-to-date methods available for the genetic manipulation of the major crops of our world

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Table of contents (32 chapters)

  1. Front Matter

    Pages i-xii
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  2. Cereals and Grasses

    1. Front Matter

      Pages 1-1
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    2. Transgenic Rice Plants

      • M. Ashikari, M. Matsuoka, S. K. Datta
      Pages 3-18

    3. Transformation of Wheat by Biolistics

      • C. A. Sparks, H. D. Jones
      Pages 19-34

    4. Genetic Transformation of Barley (Hordeum Vulgare L.) by Co-Culture of Immature Embryos with Agrobacterium

      • G. Hensel, J. Kumlehn
      Pages 35-44

    5. Maize Transformation

      • K. Wang, B. Frame
      Pages 45-62

    6. Genetic Engineering of Oat (Avena Sativa L.) Via the Biolistic Bombardment of Shoot Apical Meristems

      • S. B. Maqbool, H. Zhong, M. B. Sticklen
      Pages 63-78

    7. Generation of Transgenic Rye (Secale cereale L.) Plants with Single and Defined T-DNA Inserts, Following Agrobacterium-Mediated Gene Transfer

      • F. Altpeter, J. C. Popelka
      Pages 79-88

    8. Particle Inflow Gun-Mediated Transformation of Sorghum Bicolor

      • S. B. Williams, S. J. Gray, H. K. C. Laidlaw, I. D. Godwin
      Pages 89-102

    9. Sugarcane Transformation

      • S. J. Snyman
      Pages 103-114

    10. Biolistic Transformation of Fescues and Ryegrasses

      • G. Spangenberg, Z. Y. Wang
      Pages 115-128

  3. Woody Plants

    1. Front Matter

      Pages 129-129
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    2. Transformation of Banana using Microprojectile Bombardment

      • D. K. Becker, J. L. Dale
      Pages 131-143

    3. Agrobacterum-Mediated Transformation of Citrus

      • L. Peña, M. Cervera, C. Fagoaga, R. Pérez, J. Romero, J. Juárez et al.
      Pages 145-156

    4. Coffea SPP. Genetic Transformation

      • T. Leroy, M. Dufour
      Pages 159-169

    5. Genetic Transformation of Tea

      • A. Bhattacharya, T. K. Mondal, I. Sandal, O. Prakash, S. Kumar, P. S. Ahuja
      Pages 171-183

    6. Microprojectile-Mediated Transformation of Pineapple

      • M. R. Davey, S. Sripaoraya, P. Anthony, J. B. Power
      Pages 187-197

    7. Regeneration and Genetic Transformation of Apple (Malus SPP.)

      • S. M. W. Bulley, D. J. James
      Pages 199-214

    8. Genetic Transformation of Pear Via Agrobacterium-Mediated Gene Transfer

      • L.-H. Zhu, M. Welander
      Pages 217-228

    9. Agrobacterium-Mediated Transformation of Grape Embryogenic Calli

      • A. Perl, V. Colova-Tsolova, Y. Eshdat
      Pages 229-242
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Keywords

About this book

Since the first transgenic plants were produced back in the early 1980s, there have been substantial developments towards the genetic engineering of most crops of our world. Initial studies using isolated plant cells and removing their cell walls to form protoplasts, offered the possibility of transferring genetic material by Agrobacterium-mediated gene transfer, chemical agents or electrical charges. However, in those cases were isolated protoplasts could be transformed, often, a shoot regeneration system was not available to induce the production of transgenic plants and any such regenerated plants were subject to mutation or chromosomal of cultured plant organs, such as leaf abnormalities. By the mid-1980s, the use disks, offered the convenience of combining gene transfer, plant regeneration and selection of transformants in a single system. This approach, enabled the production of stable, phenotypically-normal, transgenic potato and tomato plants in culture. By the late 1980s, the use of biolistics offered a means of inserting foreign genes into plant cells which where inaccessible to Agrobacterium infection. Even today, this technology is now standard practice for the production of some transgenic plants.

Editors and Affiliations

  • Department of Biotechnology, National Institute of Agrobiological Sciences (NIAS), Ibaraki, Japan

    Ian S. Curtis

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