Skip to main content
SpringerLink
Log in
SpringerLink
Log in

Multiprotein Complexes

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Arnaud Poterszman

    1. Institut de Génétique et de Biologie Moléculaire et Cellulaire, Center for Integrated Biology, Integrated Structural Biology Department, Equipe labellisée Ligue Contre le Cancer, CNRS UMR 7104 - Inserm U 1258, University of Strasbourg, Illkirch, France

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2247)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 149.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Recombinant Expression and Isolation from Endogenous Source

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Production of Multi-subunit Membrane Protein Complexes

      • Burak V. Kabasakal, Qiyang Jiang, Christiane Schaffitzel
      Pages 3-16

    3. Production of Multiprotein Complexes Using the Baculovirus Expression System: Homology-Based and Restriction-Free Cloning Strategies for Construct Design

      • Paola Rossolillo, Olga Kolesnikova, Karim Essabri, Gala Ramon Zamorano, Arnaud Poterszman
      Pages 17-38

    4. Tagging Proteins with Fluorescent Reporters Using the CRISPR/Cas9 System and Double-Stranded DNA Donors

      • Sylvain Geny, Simon Pichard, Alice Brion, Jean-Baptiste Renaud, Sophie Jacquemin, Jean-Paul Concordet et al.
      Pages 39-57

    5. Validation of the Production of Antibodies in Different Formats in the HEK 293 Transient Gene Expression System

      • Jens König, Michael Hust, Joop van den Heuvel
      Pages 59-76

    6. The Intervening Removable Affinity Tag (iRAT) System for the Production of Recombinant Antibody Fragments

      • Norimichi Nomura, Yayoi Nomura, Yumi Sato, So Iwata
      Pages 77-103

    7. Isolation of Artificial Binding Proteins (Affimer Reagents) for Use in Molecular and Cellular Biology

      • Anna A. S. Tang, Christian Tiede, Michael J. McPherson, Darren C. Tomlinson
      Pages 105-121

  3. Biophysical Characterization and Sample Optimization

    1. Front Matter

      Pages 123-123
      Download chapter PDF

    2. Measurements of Protein–DNA Complexes Interactions by Isothermal Titration Calorimetry (ITC) and Microscale Thermophoresis (MST)

      • Amandine Gontier, Paloma F. Varela, Clément Nemoz, Virginie Ropars, Magali Aumont-Nicaise, Michel Desmadril et al.
      Pages 125-143

    3. switchSENSE Technology for Analysis of DNA Polymerase Kinetics

      • Guillaume Bec, Eric Ennifar
      Pages 145-153

    4. Sedimentation Velocity Methods for the Characterization of Protein Heterogeneity and Protein Affinity Interactions

      • Christine Ebel, Catherine Birck
      Pages 155-171

    5. Hands on Native Mass Spectrometry Analysis of Multi-protein Complexes

      • Stéphane Erb, Sarah Cianférani, Julien Marcoux
      Pages 173-191

    6. Studying Protein–DNA Interactions by Hydrogen/Deuterium Exchange Mass Spectrometry

      • Ruzena Filandrova, Daniel Kavan, Alan Kadek, Petr Novak, Petr Man
      Pages 193-219

    7. Integrative Mass Spectrometry–Based Approaches for Modeling Macromolecular Assemblies

      • Andy M. Lau, Argyris Politis
      Pages 221-241

    8. Optimization of Sample Preparation for the Observation of Macromolecular Complexes by Electron (cryo-)Microscopy

      • Alexandre Frechard, Grigory Sharov, Maximilien Werderer, Patrick Schultz
      Pages 243-256

    9. Solubilization and Stabilization of Native Membrane Proteins for Drug Discovery

      • Vincent Corvest, Anass Jawhari
      Pages 257-267

  4. Analysis in a Cellular Context

    1. Front Matter

      Pages 269-269
      Download chapter PDF

    2. Practical Aspects of Super-Resolution Imaging and Segmentation of Macromolecular Complexes by dSTORM

      • Leonid Andronov, Jean-Luc Vonesch, Bruno P. Klaholz
      Pages 271-286

    3. Multicolor FRET-FLIM Microscopy to Analyze Multiprotein Interactions in Live Cells

      • Abdullah Ahmed, Jennifer Schoberer, Emily Cooke, Stanley W. Botchway
      Pages 287-301
Back to top

Keywords

About this book

This volume explores strategies and detailed protocols for the preparation of macromolecular complexes and their characterization in view of structural analysis. The chapters in this book are separated into three parts: Part One focuses on sample preparation, and covers strategies for recombinant expression of multiprotein complexes in prokaryotic and eukaryotic hosts, for genome engineering using the CRISPR/Cas9 system and for production of specific binders such as reformatted antibodies and artificial binding proteins. Part Two looks at the biophysical methods that can provide useful indicators for sample optimization, and often complement structural information obtained with core technologies for structure determination—x-ray crystallography and cryo-electron microscopy—by quantitative solution data. Part Three discusses the characterization of multiprotein complexes in a cellular environment using the latest technologies and in vivo approaches. Written in the highly successfulMethods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Multiprotein Complexes: Methods and Protocols is a valuable resource for structural and molecular biologists who need to prepare multi-components for their applications, and for other scientists working on macromolecular assemblies from other angles that need to know the latest approaches that the field has to offer.


Editors and Affiliations

  • Institut de Génétique et de Biologie Moléculaire et Cellulaire, Center for Integrated Biology, Integrated Structural Biology Department, Equipe labellisée Ligue Contre le Cancer, CNRS UMR 7104 - Inserm U 1258, University of Strasbourg, Illkirch, France

    Arnaud Poterszman

Bibliographic Information

  • Book Title: Multiprotein Complexes

  • Book Subtitle: Methods and Protocols

  • Editors: Arnaud Poterszman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1126-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1125-8Published: 11 December 2020

  • Softcover ISBN: 978-1-0716-1128-9Published: 12 December 2021

  • eBook ISBN: 978-1-0716-1126-5Published: 10 December 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 340

  • Number of Illustrations: 12 b/w illustrations, 70 illustrations in colour

  • Topics: Protein Science

Publish with us

Policies and ethics

Back to top

Search

Navigation