Skip to main content
  • Book
  • © 2020

Volume Microscopy

Multiscale Imaging with Photons, Electrons, and Ions

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 155)

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (14 protocols)

  1. Front Matter

    Pages i-xvii
  2. Correlative Super Resolution and Electron Microscopy to Detect Molecules in Their Native Cellular Context

    • Tyler Ogunmowo, Sumana Raychaudhuri, Grant Kusick, Shuo Li, Shigeki Watanabe
    Pages 1-13
  3. Multicolor Superresolution Microscopy: Revealing the Nano World of Astrocytes In Situ

    • Janosch P. Heller, James P. Reynolds, Dmitri A. Rusakov
    Pages 15-35
  4. Advancing Array Tomography to Study the Fine Ultrastructure of Identified Neurons in Zebrafish (Danio rerio )

    • Marlene Strobel, Frederik Helmprobst, Martin Pauli, Manfred Heckmann, Christina Lillesaar, Christian Stigloher
    Pages 59-78
  5. A Low-Tech Approach to Serial Section Arrays

    • Waldemar Spomer, Andreas Hofmann, Lisa Veith, Ulrich Gengenbach
    Pages 79-96
  6. Large Volumes in Ultrastructural Neuropathology Imaged by Array Tomography of Routine Diagnostic Samples

    • Irene Wacker, Carsten Dittmayer, Marlene Thaler, Rasmus Schröder
    Pages 97-120
  7. Correlative Ultrastructural Analysis of Functionally Modulated Synapses Using Automated Tape-Collecting Ultramicrotome and SEM Array Tomography

    • Ye Sun, Connon Thomas, Takayasu Mikuni, Debbie Guerrero-Given, Ryohei Yasuda, Naomi Kamasawa
    Pages 121-149
  8. Improving Serial Block Face SEM by Focal Charge Compensation

    • Ann-Katrin Unger, Ralph Neujahr, Chris Hawes, Eric Hummel
    Pages 165-178
  9. FIBSEM Analysis of Interfaces Between Hard Technical Devices and Soft Neuronal Tissue

    • Antje Biesemeier, Birgit Schröppel, Wilfried Nisch, Claus J. Burkhardt
    Pages 201-220
  10. Transforming FIB-SEM Systems for Large-Volume Connectomics and Cell Biology

    • C. Shan Xu, Song Pang, Kenneth J. Hayworth, Harald F. Hess
    Pages 221-243
  11. Image Processing for Volume Electron Microscopy

    • Jörgen Kornfeld, Fabian Svara, Adrian A. Wanner
    Pages 245-262
  12. Forget About Electron Micrographs: A Novel Guide for Using 3D Models for Quantitative Analysis of Dense Reconstructions

    • Daniya J. Boges, Marco Agus, Pierre Julius Magistretti, Corrado Calì
    Pages 263-304
  13. Back Matter

    Pages 305-306

About this book

This volume discusses different approaches to workflows for large volume electron microscopy - from preparation of samples to their imaging in a variety of microscopes - in some cases also applying correlative techniques. The chapters in this book cover topics such as correlative super resolution and electron microscopy to detect molecules in their native cellular context; low-threshold access to serial section arrays; improving serial blockface SEM by focal charge compensation; FIBSEM analysis of interfaces between hard technical devices and soft neuronal tissue; and image processing for volume electron microscopy. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and authoritative, Volume Microscopy: Multiscale Imaging with Photons, Electrons, and Ions is a valuable resource for novice and expert scientists interested in learning more about this evolving field.

Editors and Affiliations

  • Cryo EM, Centre for Advanced Materials (CAM), Universität Heidelberg, Heidelberg, Germany

    Irene Wacker

  • Sector Life Science, Carl Zeiss Microscopy GmbH, Oberkochen, Germany, Carl Zeiss, Oberkochen, Germany

    Eric Hummel

  • BioQuant, Universität Heidelberg, Heidelberg, Germany, Cryo EM, BioQuant, Universitätsklinikum Heidelberg, Heidelberg, Germany

    Steffen Burgold

  • Cryo EM, Centre for Advanced Materials (CAM), Universität Heidelberg, Heidelberg, Germany, Cryo EM, BioQuant, Universitätsklinikum Heidelberg, Heidelberg, Germany

    Rasmus Schröder

Bibliographic Information

  • Book Title: Volume Microscopy

  • Book Subtitle: Multiscale Imaging with Photons, Electrons, and Ions

  • Editors: Irene Wacker, Eric Hummel, Steffen Burgold, Rasmus Schröder

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-0716-0691-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0690-2Published: 30 June 2020

  • Softcover ISBN: 978-1-0716-0693-3Published: 05 July 2020

  • eBook ISBN: 978-1-0716-0691-9Published: 29 June 2020

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XVII, 306

  • Number of Illustrations: 19 b/w illustrations, 88 illustrations in colour

  • Topics: Neurosciences, Analytical Chemistry, Materials Science, general

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access